为研究健康人外周血CD4+CD25+调节性T细胞(CD4+CD25+regulatory T cells,CD4+CD25+Tregs)在协同刺激信号作用下的扩增反应及与CD4+CD25-T细胞混合淋巴细胞反应,采用免疫磁珠法分离CD4+CD25+Tregs和CD4+CD25-T细胞,在抗CD3-mAbs和抗CD28-...为研究健康人外周血CD4+CD25+调节性T细胞(CD4+CD25+regulatory T cells,CD4+CD25+Tregs)在协同刺激信号作用下的扩增反应及与CD4+CD25-T细胞混合淋巴细胞反应,采用免疫磁珠法分离CD4+CD25+Tregs和CD4+CD25-T细胞,在抗CD3-mAbs和抗CD28-mAbs的刺激下行CD4+CD25+Tregs培养和CD4+CD25+Tregs+CD4+CD25-T细胞混合淋巴细胞培养72 h。然后加入CCK-8溶液孵育1 h,用酶标仪检测OD450值。结果为:CD4+CD25+Tregs组OD450值极显著性地低于CD4+CD25-T细胞组(P<0.01)。CD4+CD25+Tregs与CD4+CD25-T细胞混合组OD450值也极显著性地低于CD4+CD25-T细胞组(P<0.01)。CD4+CD25+Tregs表现出无反应性特征,还可抑制CD4+CD25-T细胞扩增,因此,CD4+CD25+Tregs不只是很惰性的免疫细胞,而是对保持免疫耐受发挥了积极的调节作用。展开更多
目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机...目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机分为电针治疗组、艾灸治疗组、肿瘤对照组、正常对照组。采用H_(22)肿瘤细胞移植性实体瘤模型,电针和艾灸"大椎"治疗后,磁珠分离CD4^+CD25^+Treg细胞,氚标记胸腺嘧啶核苷掺入法观察不同组小鼠CD4^+CD25^+Treg细胞体外增殖能力和针灸血清对CD4^+CD25^+ Treg细胞体外增殖的影响。结果:肿瘤对照组CD4^+CD25^+Treg细胞增殖水平比正常对照组明显增高(P<0.05);电针治疗组和艾灸治疗组CD4^+CD25^+Treg细胞增殖水平与肿瘤对照组比较均明显降低(P<0.01)。电针治疗组和艾灸治疗组1:1、1:8稀释度血清刺激正常小鼠CD4^+CD25^+Treg细胞体外增殖,较正常对照、肿瘤对照组血清显著增高(P<0.05),各组1:16、1:32稀释度血清对Treg细胞作用的差别无统计学意义(P>0.05)。结论:针灸治疗能下调荷瘤小鼠CD4^+CD25^+Treg细胞体外增殖能力。不同浓度针灸血清体外刺激CD4^+CD25^+Treg细胞增殖表现出不一致的效应。展开更多
BACKGROUND: Sepsis is a common complication ofinfections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory ...BACKGROUND: Sepsis is a common complication ofinfections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory T cells(Treg) in peripheral blood of patients with sepsis.METHODS: Periphery blood from 28 patients diagnosed with sepsis was collected on day 1 and 7 after hospitalization in the ICU of Shanghai Changzheng Hospital between December 2013 to April 2014. The blood was used for analyses of Treg ratio using flow cytometry and for analyses of blood routine test, C-reactive protein(CRP), bilirubin, procalcitonin(PCT), and coagulation. APACHE II and sequential organ failure assessment(SOFA) scores were also investigated. The results were compared between two outcome groups of survival or death to evaluate prognostic value for sepsis.RESULTS: The patients had an average age of 60.36±15.03 years, APACHE II score 16.68±7.00, and SOFA score 7.18±3.78. Among the 28 patients, 12 had severe trauma(42.9%), 10 had septic shock(35.7%), and 9(32.2%) died. The median ratio of Tregs was 2.10%(0.80%, 3.10%) in the survival group vs. 1.80%(1.15%, 3.65%) in the death group(Z=–0.148, P=0.883) on day 1; however it was signifi cantly changed to 0.90%(0.30%, 2.80%) vs. 5.70%(2.60%, 8.30%)(Z=–2.905, P=0.004).CONCLUSION: With better prospects for clinical application, dynamic monitoring of Tregs ratio in peripheral blood has potential value in predicting prognosis of sepsis.展开更多
文摘为研究健康人外周血CD4+CD25+调节性T细胞(CD4+CD25+regulatory T cells,CD4+CD25+Tregs)在协同刺激信号作用下的扩增反应及与CD4+CD25-T细胞混合淋巴细胞反应,采用免疫磁珠法分离CD4+CD25+Tregs和CD4+CD25-T细胞,在抗CD3-mAbs和抗CD28-mAbs的刺激下行CD4+CD25+Tregs培养和CD4+CD25+Tregs+CD4+CD25-T细胞混合淋巴细胞培养72 h。然后加入CCK-8溶液孵育1 h,用酶标仪检测OD450值。结果为:CD4+CD25+Tregs组OD450值极显著性地低于CD4+CD25-T细胞组(P<0.01)。CD4+CD25+Tregs与CD4+CD25-T细胞混合组OD450值也极显著性地低于CD4+CD25-T细胞组(P<0.01)。CD4+CD25+Tregs表现出无反应性特征,还可抑制CD4+CD25-T细胞扩增,因此,CD4+CD25+Tregs不只是很惰性的免疫细胞,而是对保持免疫耐受发挥了积极的调节作用。
文摘目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机分为电针治疗组、艾灸治疗组、肿瘤对照组、正常对照组。采用H_(22)肿瘤细胞移植性实体瘤模型,电针和艾灸"大椎"治疗后,磁珠分离CD4^+CD25^+Treg细胞,氚标记胸腺嘧啶核苷掺入法观察不同组小鼠CD4^+CD25^+Treg细胞体外增殖能力和针灸血清对CD4^+CD25^+ Treg细胞体外增殖的影响。结果:肿瘤对照组CD4^+CD25^+Treg细胞增殖水平比正常对照组明显增高(P<0.05);电针治疗组和艾灸治疗组CD4^+CD25^+Treg细胞增殖水平与肿瘤对照组比较均明显降低(P<0.01)。电针治疗组和艾灸治疗组1:1、1:8稀释度血清刺激正常小鼠CD4^+CD25^+Treg细胞体外增殖,较正常对照、肿瘤对照组血清显著增高(P<0.05),各组1:16、1:32稀释度血清对Treg细胞作用的差别无统计学意义(P>0.05)。结论:针灸治疗能下调荷瘤小鼠CD4^+CD25^+Treg细胞体外增殖能力。不同浓度针灸血清体外刺激CD4^+CD25^+Treg细胞增殖表现出不一致的效应。
文摘BACKGROUND: Sepsis is a common complication ofinfections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory T cells(Treg) in peripheral blood of patients with sepsis.METHODS: Periphery blood from 28 patients diagnosed with sepsis was collected on day 1 and 7 after hospitalization in the ICU of Shanghai Changzheng Hospital between December 2013 to April 2014. The blood was used for analyses of Treg ratio using flow cytometry and for analyses of blood routine test, C-reactive protein(CRP), bilirubin, procalcitonin(PCT), and coagulation. APACHE II and sequential organ failure assessment(SOFA) scores were also investigated. The results were compared between two outcome groups of survival or death to evaluate prognostic value for sepsis.RESULTS: The patients had an average age of 60.36±15.03 years, APACHE II score 16.68±7.00, and SOFA score 7.18±3.78. Among the 28 patients, 12 had severe trauma(42.9%), 10 had septic shock(35.7%), and 9(32.2%) died. The median ratio of Tregs was 2.10%(0.80%, 3.10%) in the survival group vs. 1.80%(1.15%, 3.65%) in the death group(Z=–0.148, P=0.883) on day 1; however it was signifi cantly changed to 0.90%(0.30%, 2.80%) vs. 5.70%(2.60%, 8.30%)(Z=–2.905, P=0.004).CONCLUSION: With better prospects for clinical application, dynamic monitoring of Tregs ratio in peripheral blood has potential value in predicting prognosis of sepsis.
