FPLC,SDS-PAGE and Western blot techniques are used to analyse the heterogeneity ofinterferon αA(IFN-αA) expressed in yeast.The heterogeneity consists of (i) the presence of IFN polymer,(ii)partial processing of sign...FPLC,SDS-PAGE and Western blot techniques are used to analyse the heterogeneity ofinterferon αA(IFN-αA) expressed in yeast.The heterogeneity consists of (i) the presence of IFN polymer,(ii)partial processing of signal leader peptide and (iii) internal degradation.The reasons for heterogeneity ofgene products in expression system of yeast are analysed.The methods of avoiding heterogeneity,such asdepolymerization,adding inhibitors of protease to the culture supernatant,the oligonucleotide-directed deletionmutagenesis and improvements of fermentation,are discussed.展开更多
基金the National"863"Program for the Biotechnology Development.
文摘FPLC,SDS-PAGE and Western blot techniques are used to analyse the heterogeneity ofinterferon αA(IFN-αA) expressed in yeast.The heterogeneity consists of (i) the presence of IFN polymer,(ii)partial processing of signal leader peptide and (iii) internal degradation.The reasons for heterogeneity ofgene products in expression system of yeast are analysed.The methods of avoiding heterogeneity,such asdepolymerization,adding inhibitors of protease to the culture supernatant,the oligonucleotide-directed deletionmutagenesis and improvements of fermentation,are discussed.
