Human oral bacteria live in multispecies communities in the biofilm called dental plaque. This review focuses on the interactions of seven species and the ability of each species individually and together with other s...Human oral bacteria live in multispecies communities in the biofilm called dental plaque. This review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. Community formation in biofihns in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin Gy and images are captured by confocal microscopy. Early colonizing veillonellae emerge from this review of interspecies interactions in saliva as a critical genus that guides the development of multispecies communities. Highly selective interspecies recognition is evident as initial colonizers pair with early and middle colonizers to form multispecies communities that grow on saliva.展开更多
Maintainance of sulfomucin is a key end point in the treatment of diarrhea and inflammatory bowel disease(IBD).However,the mechanisms underlying the microbial sense to sulfomucin are poorly understood,and to date,ther...Maintainance of sulfomucin is a key end point in the treatment of diarrhea and inflammatory bowel disease(IBD).However,the mechanisms underlying the microbial sense to sulfomucin are poorly understood,and to date,there are no therapies targeting the secretion and maturation of sulfomucin in IBD.Herein,we biosynthesized poly-β-hydroxybutyrate(PHB)and found that PHB could alleviate inflammation caused by diarrhea and colitis by enhancing the differentiation of sulfomucin.Microbiota transplantation and clearance together demonstrate that PHB promoting sulfomucin is mediated by Lactobacillus johnsonii(L.johnsonii).Further studies revealed that PHB provides a favorable niche for L.johnsonii biofilm formation to resist disturbance and support its growth.L.johnsonii-biofilm alleviates colitis by regulating fucose residues to promote goblet cell differentiation and subsequent sulfomucin maturation.Importantly,PHB alleviates colitis by enhancing sulfomucin secretion and maturation in a L.johnsonii-dependent manner.PHB represents a class of guardians,acting as a safe probiotic-biofilm delivery system that significantly promotes probiotic proliferation.Altogether,this study adds weight to the possible role of probiotics and functional materials in the treatment of intestinal inflammation.The application of PHB and biofilm self-coating L.johnsonii carries high translational potential and may be of clinical relevance.展开更多
N-acyl-homoserines quenching, enzymatic quenching of bacterial quorum sensing, has recently applied to mitigate biofilm in membrane bioreactor. However, the effect of AHLs on the behavior of biofilm formation is still...N-acyl-homoserines quenching, enzymatic quenching of bacterial quorum sensing, has recently applied to mitigate biofilm in membrane bioreactor. However, the effect of AHLs on the behavior of biofilm formation is still sparse. In this study, Pseudomonas aeruginosa biofilm was formed on ultra-filtration membrane under a series of N-（3-0xooxtanoyl）-L-homoserine lactone （C8-0xo-HSL） concentrations. Diffusing Cs-oxo-HSL increased the growth rate of cells on biofilm where the concentration of Cs-oxo-HSL was over 10-7 g/L. The C8-0xo-HSL gradient had no observable influence on cell density and extracellular polymeric substances of biofilm with over 10-7 g/L Cs-oxo-HSL. Surprisingly, 10-11-10-8 g/L of Cs-Oxo-HSL had no effect on cell growth in liquid culture. The cell analysis demonstrated that the quorum sensing system might enhance the growth of neighboring cells in contact with surfaces into biofilm and may influence the structure and organization of biofilm.展开更多
为探索鸡白痢沙门氏菌Ⅲ型分泌系统效应蛋白OrgC在生物被膜形成中的作用,试验以鸡白痢沙门氏菌S9及其rpoS基因缺失株S9S为母本,利用Red同源重组系统构建orgC基因缺失株并利用原核表达载体构建回复株,测定其生物被膜形成能力和对鸡肝癌(L...为探索鸡白痢沙门氏菌Ⅲ型分泌系统效应蛋白OrgC在生物被膜形成中的作用,试验以鸡白痢沙门氏菌S9及其rpoS基因缺失株S9S为母本,利用Red同源重组系统构建orgC基因缺失株并利用原核表达载体构建回复株,测定其生物被膜形成能力和对鸡肝癌(Leghorn male hepatoma,LMH)细胞黏附入侵能力。结果显示:orgC基因缺失株生物被膜形成能力与野生株S9相比无显著变化,rpoS和orgC双基因缺失株生物被膜形成能力与rpoS基因缺失株S9S相比显著降低(P<0.05);orgC基因缺失株对LMH细胞入侵能力与野生株S9相比无显著变化,rpoS和orgC双基因缺失株对LMH细胞入侵能力与rpoS基因缺失株S9S相比显著降低(P<0.05)。研究表明,Ⅲ型分泌系统效应蛋白OrgC与鸡白痢沙门氏菌生物被膜形成及细胞入侵能力有关。展开更多
文摘Human oral bacteria live in multispecies communities in the biofilm called dental plaque. This review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. Community formation in biofihns in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin Gy and images are captured by confocal microscopy. Early colonizing veillonellae emerge from this review of interspecies interactions in saliva as a critical genus that guides the development of multispecies communities. Highly selective interspecies recognition is evident as initial colonizers pair with early and middle colonizers to form multispecies communities that grow on saliva.
基金supported by the National Key Research and Development Program of China(2022YFD1300400)the National Natural Science Foundation of China(31930106 and 31829004)+3 种基金the Chinese Universities Scientific Fund(2022TC033)the 2115 Talent Development Program of China Agricultural University(1041-00109019)the Henan Province Public Benefit Research Foundation(201300111200-05)the 111 Project(B16044)。
文摘Maintainance of sulfomucin is a key end point in the treatment of diarrhea and inflammatory bowel disease(IBD).However,the mechanisms underlying the microbial sense to sulfomucin are poorly understood,and to date,there are no therapies targeting the secretion and maturation of sulfomucin in IBD.Herein,we biosynthesized poly-β-hydroxybutyrate(PHB)and found that PHB could alleviate inflammation caused by diarrhea and colitis by enhancing the differentiation of sulfomucin.Microbiota transplantation and clearance together demonstrate that PHB promoting sulfomucin is mediated by Lactobacillus johnsonii(L.johnsonii).Further studies revealed that PHB provides a favorable niche for L.johnsonii biofilm formation to resist disturbance and support its growth.L.johnsonii-biofilm alleviates colitis by regulating fucose residues to promote goblet cell differentiation and subsequent sulfomucin maturation.Importantly,PHB alleviates colitis by enhancing sulfomucin secretion and maturation in a L.johnsonii-dependent manner.PHB represents a class of guardians,acting as a safe probiotic-biofilm delivery system that significantly promotes probiotic proliferation.Altogether,this study adds weight to the possible role of probiotics and functional materials in the treatment of intestinal inflammation.The application of PHB and biofilm self-coating L.johnsonii carries high translational potential and may be of clinical relevance.
基金supported by the National High Technology Research and Development Program(863)of China(No.2009AA062902)
文摘N-acyl-homoserines quenching, enzymatic quenching of bacterial quorum sensing, has recently applied to mitigate biofilm in membrane bioreactor. However, the effect of AHLs on the behavior of biofilm formation is still sparse. In this study, Pseudomonas aeruginosa biofilm was formed on ultra-filtration membrane under a series of N-（3-0xooxtanoyl）-L-homoserine lactone （C8-0xo-HSL） concentrations. Diffusing Cs-oxo-HSL increased the growth rate of cells on biofilm where the concentration of Cs-oxo-HSL was over 10-7 g/L. The C8-0xo-HSL gradient had no observable influence on cell density and extracellular polymeric substances of biofilm with over 10-7 g/L Cs-oxo-HSL. Surprisingly, 10-11-10-8 g/L of Cs-Oxo-HSL had no effect on cell growth in liquid culture. The cell analysis demonstrated that the quorum sensing system might enhance the growth of neighboring cells in contact with surfaces into biofilm and may influence the structure and organization of biofilm.
文摘为探索鸡白痢沙门氏菌Ⅲ型分泌系统效应蛋白OrgC在生物被膜形成中的作用,试验以鸡白痢沙门氏菌S9及其rpoS基因缺失株S9S为母本,利用Red同源重组系统构建orgC基因缺失株并利用原核表达载体构建回复株,测定其生物被膜形成能力和对鸡肝癌(Leghorn male hepatoma,LMH)细胞黏附入侵能力。结果显示:orgC基因缺失株生物被膜形成能力与野生株S9相比无显著变化,rpoS和orgC双基因缺失株生物被膜形成能力与rpoS基因缺失株S9S相比显著降低(P<0.05);orgC基因缺失株对LMH细胞入侵能力与野生株S9相比无显著变化,rpoS和orgC双基因缺失株对LMH细胞入侵能力与rpoS基因缺失株S9S相比显著降低(P<0.05)。研究表明,Ⅲ型分泌系统效应蛋白OrgC与鸡白痢沙门氏菌生物被膜形成及细胞入侵能力有关。
