目的探讨重庆市大气细颗粒物(PM2.5)中的多环芳烃(polycyclic aromatic hydrocarbons,PAHs)暴露对男性精子DNA的损害效应。方法研究对象来源于2013、2014年重庆市大学生男性生殖健康研究队列(male reproductive health in Chongqing col...目的探讨重庆市大气细颗粒物(PM2.5)中的多环芳烃(polycyclic aromatic hydrocarbons,PAHs)暴露对男性精子DNA的损害效应。方法研究对象来源于2013、2014年重庆市大学生男性生殖健康研究队列(male reproductive health in Chongqing college students,MARHCS),检测采样期间大气PM2.5中的低环PAHs(2~4环)和高环PAHs(5~6环)各组分的含量;采用单细胞凝胶电泳实验检测精子DNA损伤;采用混合效应模型探讨PAHs各组分的暴露程度与男性大学生精子DNA损伤水平之间的关联。结果2013年大气细颗粒物中的PAHs组分以低环PAHs为主,其中菲、芴、芘的占比最高,而2014年大气细颗粒物中则以高环PAHs为主,如茚并[1,2,3-cd]芘、苯并[g,h,i]苝、苯并[a]芘等。在2014年,男性大学生的精子DNA损伤指标如彗星尾长、尾矩和Olive尾矩较2013年明显增加(P<0.05)。混合效应模型结果显示:低环PAHs中的荧蒽、苊烯、芘和总低环PAHs与精子DNA损伤指标中的彗星尾长和尾矩呈负相关。高环PAHs中的苯并[k]荧蒽、苯并[b]荧蒽和二苯并[a,h]蒽与精子DNA损伤指标中的彗星尾长呈正相关[β(95%CI):108.987(43.754~203.820)、158.700(65.957~303.272)、185.708(67.350~387.775),校正P均<0.05];与彗星尾矩呈正相关[β(95%CI):449.204(260.066~737.695)、683.108(374.515~1192.387)、683.108(374.515~1192.387),校正P均<0.05]。结论重庆市大气细颗粒物中的高环PAHs组分暴露,可能导致精子DNA损伤效应增加。展开更多
目的:研究大气细颗粒物对小鼠皮肤组织表皮细胞生长因子受体(epidermal growth factor receptor,EGFR)m RNA表达的影响,以探讨大气细颗粒物对皮肤老化的影响。方法:将40只BALB/c雌性小鼠随机分成生理盐水对照组及大气细颗粒物(PM2.5)染...目的:研究大气细颗粒物对小鼠皮肤组织表皮细胞生长因子受体(epidermal growth factor receptor,EGFR)m RNA表达的影响,以探讨大气细颗粒物对皮肤老化的影响。方法:将40只BALB/c雌性小鼠随机分成生理盐水对照组及大气细颗粒物(PM2.5)染毒低、中、高剂量(分别为1.6、8.0和40.0mg/kg)组,每组10只。各剂量组均经气管滴注染毒3d。末次染毒24h后,采用实时荧光定量PCR方法分别检测各组小鼠皮肤组织EGFR m RNA的表达量。结果:与对照组相比,PM2.5染毒中、高剂量组EGFR m RNA的表达量均明显升高(P<0.05),且与剂量呈正相关。结论:中、高浓度PM2.5可以诱导EGFR m RNA的表达,促进皮肤老化的发生。展开更多
[Objective] The aim was to study the concentration characteristics and sources of chemical elements in atmospheric fine particles (PM2.5) in autumn in Xi’an City. [Method] By means of mini-volume sampler, PM2.5 sampl...[Objective] The aim was to study the concentration characteristics and sources of chemical elements in atmospheric fine particles (PM2.5) in autumn in Xi’an City. [Method] By means of mini-volume sampler, PM2.5 samples in atmosphere in Xi’an were collected in October 2009, and the concentration characteristics and sources of elements in PM2.5 were analyzed. [Result] The average mass concentration of PM2.5 in atmosphere in autumn in Xi’an City was 168.44 μg/m3 which was higher than that of Beijing and Pearl River Delta area, and the minimum and maximum value were 53.29 and 358.16 μg/m3, respectively. The mass concentration of S, Zn, K, Cl, Ca and Fe in PM2.5 was above 1.0 μg/m3, being at high pollution level. In addition, K had obvious correlation with organic carbon (OC) and element carbon (EC), with the correlation coefficients of 0.76 and 0.75 (P<0.000 1), respectively, and it showed that OC and EC had the same source as K, namely biomass burning had certain contribution to OC and EC. Enrichment factors analysis revealed that K, Ca, Fe, Ti, Mn and Cr mainly came from earth crust, rock weathering and other natural sources, while anthropogenic pollution sources had great effects on S, Zn, Cl, Pb, Br, Mo, Cd and As which were affected by soil dust and other natural sources slightly, and Cd had the highest enrichment factor and mainly came from metal smelting. In a word, coal combustion, biomass burning, vehicle emissions, metallurgical, chemical industry and dust were the main sources of PM2.5 in autumn in Xi’an. [Conclusion] The study could provide theoretical foundation for the control of urban environmental pollution.展开更多
文摘目的探讨重庆市大气细颗粒物(PM2.5)中的多环芳烃(polycyclic aromatic hydrocarbons,PAHs)暴露对男性精子DNA的损害效应。方法研究对象来源于2013、2014年重庆市大学生男性生殖健康研究队列(male reproductive health in Chongqing college students,MARHCS),检测采样期间大气PM2.5中的低环PAHs(2~4环)和高环PAHs(5~6环)各组分的含量;采用单细胞凝胶电泳实验检测精子DNA损伤;采用混合效应模型探讨PAHs各组分的暴露程度与男性大学生精子DNA损伤水平之间的关联。结果2013年大气细颗粒物中的PAHs组分以低环PAHs为主,其中菲、芴、芘的占比最高,而2014年大气细颗粒物中则以高环PAHs为主,如茚并[1,2,3-cd]芘、苯并[g,h,i]苝、苯并[a]芘等。在2014年,男性大学生的精子DNA损伤指标如彗星尾长、尾矩和Olive尾矩较2013年明显增加(P<0.05)。混合效应模型结果显示:低环PAHs中的荧蒽、苊烯、芘和总低环PAHs与精子DNA损伤指标中的彗星尾长和尾矩呈负相关。高环PAHs中的苯并[k]荧蒽、苯并[b]荧蒽和二苯并[a,h]蒽与精子DNA损伤指标中的彗星尾长呈正相关[β(95%CI):108.987(43.754~203.820)、158.700(65.957~303.272)、185.708(67.350~387.775),校正P均<0.05];与彗星尾矩呈正相关[β(95%CI):449.204(260.066~737.695)、683.108(374.515~1192.387)、683.108(374.515~1192.387),校正P均<0.05]。结论重庆市大气细颗粒物中的高环PAHs组分暴露,可能导致精子DNA损伤效应增加。
文摘目的:研究大气细颗粒物对小鼠皮肤组织表皮细胞生长因子受体(epidermal growth factor receptor,EGFR)m RNA表达的影响,以探讨大气细颗粒物对皮肤老化的影响。方法:将40只BALB/c雌性小鼠随机分成生理盐水对照组及大气细颗粒物(PM2.5)染毒低、中、高剂量(分别为1.6、8.0和40.0mg/kg)组,每组10只。各剂量组均经气管滴注染毒3d。末次染毒24h后,采用实时荧光定量PCR方法分别检测各组小鼠皮肤组织EGFR m RNA的表达量。结果:与对照组相比,PM2.5染毒中、高剂量组EGFR m RNA的表达量均明显升高(P<0.05),且与剂量呈正相关。结论:中、高浓度PM2.5可以诱导EGFR m RNA的表达,促进皮肤老化的发生。
基金Supported by West Light Joint Scholar Project of Chinese Academy of Sciences
文摘[Objective] The aim was to study the concentration characteristics and sources of chemical elements in atmospheric fine particles (PM2.5) in autumn in Xi’an City. [Method] By means of mini-volume sampler, PM2.5 samples in atmosphere in Xi’an were collected in October 2009, and the concentration characteristics and sources of elements in PM2.5 were analyzed. [Result] The average mass concentration of PM2.5 in atmosphere in autumn in Xi’an City was 168.44 μg/m3 which was higher than that of Beijing and Pearl River Delta area, and the minimum and maximum value were 53.29 and 358.16 μg/m3, respectively. The mass concentration of S, Zn, K, Cl, Ca and Fe in PM2.5 was above 1.0 μg/m3, being at high pollution level. In addition, K had obvious correlation with organic carbon (OC) and element carbon (EC), with the correlation coefficients of 0.76 and 0.75 (P<0.000 1), respectively, and it showed that OC and EC had the same source as K, namely biomass burning had certain contribution to OC and EC. Enrichment factors analysis revealed that K, Ca, Fe, Ti, Mn and Cr mainly came from earth crust, rock weathering and other natural sources, while anthropogenic pollution sources had great effects on S, Zn, Cl, Pb, Br, Mo, Cd and As which were affected by soil dust and other natural sources slightly, and Cd had the highest enrichment factor and mainly came from metal smelting. In a word, coal combustion, biomass burning, vehicle emissions, metallurgical, chemical industry and dust were the main sources of PM2.5 in autumn in Xi’an. [Conclusion] The study could provide theoretical foundation for the control of urban environmental pollution.
