[A Review] The process of apoptosis is very complex.Apoptosis-inducing factor(AIF)has the ability to induce apoptosis via a caspase-independent pathway.The mature AIF protein,a flavoprotein(prosthetic group:flavine ad...[A Review] The process of apoptosis is very complex.Apoptosis-inducing factor(AIF)has the ability to induce apoptosis via a caspase-independent pathway.The mature AIF protein,a flavoprotein(prosthetic group:flavine adenine dinucleotide)is normally confined to the mitochondrial intermembrane space.AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus.AIF induces nuclear chromatin condensation,as well as large scale(V50 kb)DNA fragmentation.AIF is a phyloge-netically old,bifunctional protein with an electron acceptor/donor(oxidoreductase)function and a second apoptogenic function and AIF acts in a caspase-independent fashion.The molecular mechanisms via which AIF induces apoptosis are discussed in this review.展开更多
目的研究羟基喜树碱诱导肝癌细胞株SMMC-7721凋亡时线粒体凋亡相关蛋白凋亡诱导因子表达及从线粒体发生核转位的变化。方法用80μ／ml羟基喜树碱作用于肝癌细胞株SMMC-7721后, 用吖啶橙／溴化乙啶染色法观察细胞凋亡现象;用电子显微镜...目的研究羟基喜树碱诱导肝癌细胞株SMMC-7721凋亡时线粒体凋亡相关蛋白凋亡诱导因子表达及从线粒体发生核转位的变化。方法用80μ／ml羟基喜树碱作用于肝癌细胞株SMMC-7721后, 用吖啶橙／溴化乙啶染色法观察细胞凋亡现象;用电子显微镜观察线粒体超微结构;分别用逆转录聚合酶链反应、Western blot检测凋亡诱导因子在mRNA与蛋白质水平表达的变化;用激光共聚焦显微镜观察凋亡诱导因子在细胞凋亡时从线粒体到核的迁移变化。结果 80μg／ml羟基喜树碱作用SMMC-7721细胞后, 吖啶橙／溴化乙啶荧光双重染色可见细胞体积缩小、细胞皱缩、核碎裂等典型细胞凋亡形态学改变;超微结构观察发现线粒体肿胀;细胞凋亡时凋亡诱导因子在m R N A与蛋白质水平上的表达与对照组细胞相比没有明显变化,但凋亡诱导因子发生了从线粒体到核的迁移。结论羟基喜树碱可以通过线粒体途径诱导人肝癌细胞发生凋亡;在线粒体凋亡途径中,线粒体凋亡相关蛋白凋亡诱导因子从线粒体释放并发生核转位可能与羟基喜树碱诱导细胞凋亡密切相关。展开更多
文摘[A Review] The process of apoptosis is very complex.Apoptosis-inducing factor(AIF)has the ability to induce apoptosis via a caspase-independent pathway.The mature AIF protein,a flavoprotein(prosthetic group:flavine adenine dinucleotide)is normally confined to the mitochondrial intermembrane space.AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus.AIF induces nuclear chromatin condensation,as well as large scale(V50 kb)DNA fragmentation.AIF is a phyloge-netically old,bifunctional protein with an electron acceptor/donor(oxidoreductase)function and a second apoptogenic function and AIF acts in a caspase-independent fashion.The molecular mechanisms via which AIF induces apoptosis are discussed in this review.
文摘目的研究羟基喜树碱诱导肝癌细胞株SMMC-7721凋亡时线粒体凋亡相关蛋白凋亡诱导因子表达及从线粒体发生核转位的变化。方法用80μ／ml羟基喜树碱作用于肝癌细胞株SMMC-7721后, 用吖啶橙／溴化乙啶染色法观察细胞凋亡现象;用电子显微镜观察线粒体超微结构;分别用逆转录聚合酶链反应、Western blot检测凋亡诱导因子在mRNA与蛋白质水平表达的变化;用激光共聚焦显微镜观察凋亡诱导因子在细胞凋亡时从线粒体到核的迁移变化。结果 80μg／ml羟基喜树碱作用SMMC-7721细胞后, 吖啶橙／溴化乙啶荧光双重染色可见细胞体积缩小、细胞皱缩、核碎裂等典型细胞凋亡形态学改变;超微结构观察发现线粒体肿胀;细胞凋亡时凋亡诱导因子在m R N A与蛋白质水平上的表达与对照组细胞相比没有明显变化,但凋亡诱导因子发生了从线粒体到核的迁移。结论羟基喜树碱可以通过线粒体途径诱导人肝癌细胞发生凋亡;在线粒体凋亡途径中,线粒体凋亡相关蛋白凋亡诱导因子从线粒体释放并发生核转位可能与羟基喜树碱诱导细胞凋亡密切相关。
