Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The pr...Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of展开更多
Background Apolipoprotein （apo） A-V is a novel member of the apolipoprotein cluster involved in triacylglycerol （TG） homeostasis. It has reported that APOA5 gene polymorphisms is correlated with arteriosclerotic d...Background Apolipoprotein （apo） A-V is a novel member of the apolipoprotein cluster involved in triacylglycerol （TG） homeostasis. It has reported that APOA5 gene polymorphisms is correlated with arteriosclerotic diseases. However, This association is unknown on Chinese patients with atherosclerotic cerebral infarction. The present study aimed to elucidate the relationship of APOA5 -1131T 〉 C and arteriosclerotic cerebral infarction （ACI） as well as the levels of serum lipids. Methods Polymerase chain reaction-restriction fragments length polymorphisms （PCR-RFLP） analysis, enzymatic and immunoturbidimetry methods were used to measure- 1131T 〉 C genotype, allele frequency as well as plasma lipid level of 90 ACI patients and 221 healthy subjects of Han Chinese. Results In ACI group, the level of TG in allele C carriers was higher than that of non-C carriers （P 〈 0.05）. The frequency of allele C in ACI group was higher than in healthy group （~2 = 5.568, P = 0.018）. Except sex, age and BMI, the levels of total cholesterol （TC）, triglycerides （TG）, high density lipoprotein choles- terol （HDL-C）, low-density lipoprotein cholesterol （LDL-C）, APOA1 and APOB in ACI group distinctively were higher than those in healthy group. Conclusion The APOA5-1131 allele C is associated with the high level of TG in ACI patients, which is probably linked with ACI danger of Chinese Han.展开更多
文摘Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of
基金supported by the grants from Hubei Provincial Department of Education(No.D20122406)
文摘Background Apolipoprotein （apo） A-V is a novel member of the apolipoprotein cluster involved in triacylglycerol （TG） homeostasis. It has reported that APOA5 gene polymorphisms is correlated with arteriosclerotic diseases. However, This association is unknown on Chinese patients with atherosclerotic cerebral infarction. The present study aimed to elucidate the relationship of APOA5 -1131T 〉 C and arteriosclerotic cerebral infarction （ACI） as well as the levels of serum lipids. Methods Polymerase chain reaction-restriction fragments length polymorphisms （PCR-RFLP） analysis, enzymatic and immunoturbidimetry methods were used to measure- 1131T 〉 C genotype, allele frequency as well as plasma lipid level of 90 ACI patients and 221 healthy subjects of Han Chinese. Results In ACI group, the level of TG in allele C carriers was higher than that of non-C carriers （P 〈 0.05）. The frequency of allele C in ACI group was higher than in healthy group （~2 = 5.568, P = 0.018）. Except sex, age and BMI, the levels of total cholesterol （TC）, triglycerides （TG）, high density lipoprotein choles- terol （HDL-C）, low-density lipoprotein cholesterol （LDL-C）, APOA1 and APOB in ACI group distinctively were higher than those in healthy group. Conclusion The APOA5-1131 allele C is associated with the high level of TG in ACI patients, which is probably linked with ACI danger of Chinese Han.
