为揭示小麦自然群体干旱胁迫条件下旗叶叶绿素含量的变化,筛选相关标记的优异等位变异,以262份小麦种质资源组成的自然群体为材料,分别种植在北京的2个试验地点,均设雨养和灌溉处理,于开花期和灌浆期检测旗叶叶绿素含量。以分布于21条...为揭示小麦自然群体干旱胁迫条件下旗叶叶绿素含量的变化,筛选相关标记的优异等位变异,以262份小麦种质资源组成的自然群体为材料,分别种植在北京的2个试验地点,均设雨养和灌溉处理,于开花期和灌浆期检测旗叶叶绿素含量。以分布于21条染色体的169个SSR标记检测所有材料的基因型,利用STRUCTURE2.3.2软件分析群体结构,用TASSEL软件的MLM(mixed linear model)方法对小麦自然群体的旗叶叶绿素含量进行关联分析。在此基础上,将携带某等位变异的所有材料表型均值与携带无效等位基因(null allele)材料表型均值比较,估计等位变异的表型效应,鉴别优异等位变异。共检测到2048个等位变异,每位点2～37个等位变异,平均12个。每位点的标记多态性信息量(PIC)为0.008～0.936,平均0.628。在22个标记位点共检测出40个(次)与旗叶叶绿素含量极显著的关联,其中11个标记位点有2次以上的关联,Xwmc419-1B和Xgwm501-2B分别有3次关联。在Xcfa2123-7A、Xgwm232-1D和Xgwm429-2B位点分别检测到效应值大于4.0的等位变异。展开更多
Allelic variation in two domestic wheat landraces, Pingyaobaimai and Mazhamai, two cornerstone breeding materials and their derived cultivars with drought tolerance was detected by SSR (simple sequence repeat) markers...Allelic variation in two domestic wheat landraces, Pingyaobaimai and Mazhamai, two cornerstone breeding materials and their derived cultivars with drought tolerance was detected by SSR (simple sequence repeat) markers. The clustering of 25 accessions showed that the similarity between Pingyaobaimai and Yandal817, the latter was developed from the former, was 0.71, the highest one of all accessions, but the similarities were very low between these two accessions and other accessions including their derived cultivars. A similar situation was revealed between Mazhamai and its derived cultivars. Pingyaobaimai and its three derived cultivars shared three alleles at loci Xgwm526, Xgwm538 and Xgwm126 on chromosome arms 2BL, 4BL and 5AL, respectively. There were six shared alleles in Mazhamai and its derived cultivars, in order of Xgwm157, Xgwm126, Xgwm212, Xgwm626, Xgwm471 and Xgwm44 on chromosome arms 2DL, 5AL, 5DL, 6BL, 7AS and 7DC, respectively. Only one shared allele was detected between the pedigrees of Pingyaobaimai and Mazhamai. The difference of shared alleles in two cornerstone breeding materials and their derived cultivars revealed the diversity in Chinese wheat germplasm with drought tolerance and the complication in genetic basis of drought tolerance in wheat.展开更多
本研究检测了214个陆地棉材料在多环境下的纤维品质指标(上半部平均长度、断裂比强度、马克隆值、伸长率和整齐度),选用在棉花基因组上均匀分布多态性较好的259个SSR标记对供试群体进行基因型检测,利用Tassel软件中的GLM(Q)方法挖掘与...本研究检测了214个陆地棉材料在多环境下的纤维品质指标(上半部平均长度、断裂比强度、马克隆值、伸长率和整齐度),选用在棉花基因组上均匀分布多态性较好的259个SSR标记对供试群体进行基因型检测,利用Tassel软件中的GLM(Q)方法挖掘与纤维品质指标相关的QTLs,依据表型效应值鉴别优异等位变异位点及典型材料。结果显示:同一纤维品质性状在3个地点2~3年内变化趋势相对稳定,纤维上半部平均长度、断裂比强度和整齐度三者之间呈正相关(P<0.01),纤维上半部平均长度/断裂比强度均和马克隆值/伸长率负相关。259个SSR标记共检测到309个等位基因,涉及774个基因型,多态性信息含量(PIC,polymorphic information content)平均为0.2688,基因多样性指数平均为0.2239。按照基因型数据可将该群体划分为2个亚群。通过关联分析获得与纤维品质相关的等位变异位点134个(P<0.01),在3个及以上环境中均可检测到的位点有30个,有3个位点(NAU6177、DPL0886、NAU3607)在7个环境中分别与断裂比强度、马克隆值、伸长率显著关联(P<0.01),最高表型变异解释率分别为11.14%、5.74%和13.99%。31个位点同时与2个及以上纤维品质指标相关,其中,NAU 6177与5个纤维品质指标均显著关联(P<0.01)。与已发表结果比对,17个QTL已被报道与纤维品质性状相关,10个QTL与前人关联指标相同。分析多环境纤维品质关联位点的表型效应,获得72个等位变异位点,5份携带优异等位基因载体材料。本研究在多环境下挖掘与陆地棉纤维品质指标关联的分子标记,同时鉴别携带优异等位变异基因的典型载体材料,为棉花纤维品质分子标记辅助选择及目标基因定位提供有益信息。展开更多
文摘为揭示小麦自然群体干旱胁迫条件下旗叶叶绿素含量的变化,筛选相关标记的优异等位变异,以262份小麦种质资源组成的自然群体为材料,分别种植在北京的2个试验地点,均设雨养和灌溉处理,于开花期和灌浆期检测旗叶叶绿素含量。以分布于21条染色体的169个SSR标记检测所有材料的基因型,利用STRUCTURE2.3.2软件分析群体结构,用TASSEL软件的MLM(mixed linear model)方法对小麦自然群体的旗叶叶绿素含量进行关联分析。在此基础上,将携带某等位变异的所有材料表型均值与携带无效等位基因(null allele)材料表型均值比较,估计等位变异的表型效应,鉴别优异等位变异。共检测到2048个等位变异,每位点2～37个等位变异,平均12个。每位点的标记多态性信息量(PIC)为0.008～0.936,平均0.628。在22个标记位点共检测出40个(次)与旗叶叶绿素含量极显著的关联,其中11个标记位点有2次以上的关联,Xwmc419-1B和Xgwm501-2B分别有3次关联。在Xcfa2123-7A、Xgwm232-1D和Xgwm429-2B位点分别检测到效应值大于4.0的等位变异。
文摘Allelic variation in two domestic wheat landraces, Pingyaobaimai and Mazhamai, two cornerstone breeding materials and their derived cultivars with drought tolerance was detected by SSR (simple sequence repeat) markers. The clustering of 25 accessions showed that the similarity between Pingyaobaimai and Yandal817, the latter was developed from the former, was 0.71, the highest one of all accessions, but the similarities were very low between these two accessions and other accessions including their derived cultivars. A similar situation was revealed between Mazhamai and its derived cultivars. Pingyaobaimai and its three derived cultivars shared three alleles at loci Xgwm526, Xgwm538 and Xgwm126 on chromosome arms 2BL, 4BL and 5AL, respectively. There were six shared alleles in Mazhamai and its derived cultivars, in order of Xgwm157, Xgwm126, Xgwm212, Xgwm626, Xgwm471 and Xgwm44 on chromosome arms 2DL, 5AL, 5DL, 6BL, 7AS and 7DC, respectively. Only one shared allele was detected between the pedigrees of Pingyaobaimai and Mazhamai. The difference of shared alleles in two cornerstone breeding materials and their derived cultivars revealed the diversity in Chinese wheat germplasm with drought tolerance and the complication in genetic basis of drought tolerance in wheat.
文摘本研究检测了214个陆地棉材料在多环境下的纤维品质指标(上半部平均长度、断裂比强度、马克隆值、伸长率和整齐度),选用在棉花基因组上均匀分布多态性较好的259个SSR标记对供试群体进行基因型检测,利用Tassel软件中的GLM(Q)方法挖掘与纤维品质指标相关的QTLs,依据表型效应值鉴别优异等位变异位点及典型材料。结果显示:同一纤维品质性状在3个地点2~3年内变化趋势相对稳定,纤维上半部平均长度、断裂比强度和整齐度三者之间呈正相关(P<0.01),纤维上半部平均长度/断裂比强度均和马克隆值/伸长率负相关。259个SSR标记共检测到309个等位基因,涉及774个基因型,多态性信息含量(PIC,polymorphic information content)平均为0.2688,基因多样性指数平均为0.2239。按照基因型数据可将该群体划分为2个亚群。通过关联分析获得与纤维品质相关的等位变异位点134个(P<0.01),在3个及以上环境中均可检测到的位点有30个,有3个位点(NAU6177、DPL0886、NAU3607)在7个环境中分别与断裂比强度、马克隆值、伸长率显著关联(P<0.01),最高表型变异解释率分别为11.14%、5.74%和13.99%。31个位点同时与2个及以上纤维品质指标相关,其中,NAU 6177与5个纤维品质指标均显著关联(P<0.01)。与已发表结果比对,17个QTL已被报道与纤维品质性状相关,10个QTL与前人关联指标相同。分析多环境纤维品质关联位点的表型效应,获得72个等位变异位点,5份携带优异等位基因载体材料。本研究在多环境下挖掘与陆地棉纤维品质指标关联的分子标记,同时鉴别携带优异等位变异基因的典型载体材料,为棉花纤维品质分子标记辅助选择及目标基因定位提供有益信息。
