摘要
分析大豆亲本材料的遗传多样性,发掘农艺性状关联位点的优异等位变异,为大豆杂交组合的配置及分子标记辅助育种提供依据。本试验利用59个SSR标记对90份大豆种质资源进行多态性扫描和遗传多样性分析。筛选出46个标记,应用STRUCTURE2.3.2软件进行群体结构遗传分析,利用Tassel2.1软件MLM模型对18个农艺性状进行关联分析,发掘优异等位变异。结果表明：（1）59个标记中50个标记具有多态性,共检测出154个等位变异;多态性信息值PIC分布范围为0.042~0.765,平均PIC=0.421;SSR标记位点的Shannon指数（H’）的分布范围为0.1066~1.6804,平均值为0.8241;遗传距离的变异范围为0.0307~1.4529,平均值0.7015。（2）供试材料分为4个亚群。发现7个与分枝数、百粒重、叶形、主茎节数、生育期和蛋白含量相关联的标记,表型变异的解释率为0.003~0.339。研究在各关联位点找到了Satt263-A279、Satt156-A203、Satt567-A112等表型效应明显的优异等位变异。为大豆育种优异基因的克隆提供科学依据。
In order to analyze the genetic diversity of parent materials, discover elite alleles associated with agronomic traits and provide useful information for hybridization combination of parent materials and molecular assisted breeding, the genetic diversity of 90 soybean cuhivars was determined by using 59 simple sequence repeat （SSR） markers. The genotyping data of 46 SSR markers selected was used to analyze population structure, and then association analysis between SSR loci and 18 soybean agronomic traits was performed to discover elite alleles using TASSEL2. 1 MLM （Mixed Linear Model） program. The results showed： （1） A total of 154 alleles were found from 50 loci which appeared to be polymorphic among the 59 markers, while the polymorphism information content （PIC） values varied from 0. 042 to 0. 765 with the mean of 0. 521 and Shannon index varied from 0. 1066 to 1. 6804 with the mean of 0. 8241. Genetic distance ranged from 0.0307 to 1.4529, with the mean of 0.7015. Population structure analysis showed that the population of these materials was composed of 4 subpopulations. 7 SSR loci were found to associate with branch number, weight of 100 seeds, growth period, node number of main stem, leaf shape and protein content with the percentage of variation for the phenotype explained by the related markers ranging from 0. 3% to 33.9%. Elite allelic genes, such as Satt263-A279, Satt156 -A203, Satt567 -A112 and so on, were determined by analyzing the relationship between different allelic variation genes of associated loci and phenotype effects. These results provide evidence for cloning high quality genes and can be further utilized in breeding programs.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第9期1576-1584,共9页
Journal of Nuclear Agricultural Sciences
基金
山西省科技攻关项目(20120311005-3)
山西省农业科技成果转化资金项目
山西农业大学科技创新基金项目(育种基金)(2006057)
关键词
大豆
SSR
遗传多样性
群体结构
关联分析
等位变异
Soybean
SSR
Genetic diversity
Population structure
Association analysis
Allelic variation
