To gain insight into how anthocyanin biosynthesis is controlled by light in fruit,transcriptome and metabolome analyses were performed in the Chinese sand pear cultivar“Mantianhong”(Pyrus pyrifolia)after bagging and...To gain insight into how anthocyanin biosynthesis is controlled by light in fruit,transcriptome and metabolome analyses were performed in the Chinese sand pear cultivar“Mantianhong”(Pyrus pyrifolia)after bagging and bag removal.We investigated transcriptional and metabolic changes and gene-metabolite correlation networks.Correlation tests of anthocyanin content and transcriptional changes revealed that 1,530 transcripts were strongly correlated with 15 anthocyanin derivatives(R2>0.9,P-value<0.05),with the top 130 transcripts categorized as being associated with flavonoid metabolism,transcriptional regulation,and light signaling.The connection network revealed a new photosensitive transcription factor,PybZIPa,that might play an important role during light-induced anthocyanin accumulation.The overexpression of PybZIPa promoted anthocyanin accumulation in pear and strawberry fruit as well as tobacco leaves.Dual luciferase and Y1H assays further verified that PybZIPa directly activated the expression of PyUFGT by binding to tandem G-box motifs in the promoter,which was key to differential anthocyanin accumulation in debagged pear skin,and the number of G-box motifs affected the transcriptional activation of PyUFGT by PybZIPa.The results indicate that the light-induced anthocyanin biosynthesis regulatory mechanism in pear differs from that described in previous reports suggesting that a bZIP family member co-regulates anthocyanin biosynthesis with other transcription factors in apple and Arabidopsis.It was found that,in response to light,PybZIPa promoted anthocyanin biosynthesis by regulating important transcription factors(PyMYB114,PyMYB10,and PyBBX22)as well as structural genes(PyUFGT)via binding to G-boxes within promoters.This activation was amplified by the self-binding of PybZIPa to activate its own promoter.Overall,we demonstrate the utility of a multiomics integrative approach for discovering new functional genes and pathways underlying light-induced anthocyanin biosynthesis.展开更多
The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be ...The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be radiologically activated prior to treatment and“coerce”tumor cells into readily absorb these nanoparticles.This targeted intracytoplasmic delivery of short-lived radiologically activated nanoparticles could provide less“whole-body”radiation dose while delivering a short lived potent tumor localized dose,along with their low toxicity may prove to be another tool in the treatment of diverse cancers.展开更多
基金funded by the National Science Foundation of China(31820103012 and 31672111)the Earmarked Fund for China Agriculture Research System(CARS-28)the“333 High Level Talents Project”of Jiangsu Province(BRA2016367).
文摘To gain insight into how anthocyanin biosynthesis is controlled by light in fruit,transcriptome and metabolome analyses were performed in the Chinese sand pear cultivar“Mantianhong”(Pyrus pyrifolia)after bagging and bag removal.We investigated transcriptional and metabolic changes and gene-metabolite correlation networks.Correlation tests of anthocyanin content and transcriptional changes revealed that 1,530 transcripts were strongly correlated with 15 anthocyanin derivatives(R2>0.9,P-value<0.05),with the top 130 transcripts categorized as being associated with flavonoid metabolism,transcriptional regulation,and light signaling.The connection network revealed a new photosensitive transcription factor,PybZIPa,that might play an important role during light-induced anthocyanin accumulation.The overexpression of PybZIPa promoted anthocyanin accumulation in pear and strawberry fruit as well as tobacco leaves.Dual luciferase and Y1H assays further verified that PybZIPa directly activated the expression of PyUFGT by binding to tandem G-box motifs in the promoter,which was key to differential anthocyanin accumulation in debagged pear skin,and the number of G-box motifs affected the transcriptional activation of PyUFGT by PybZIPa.The results indicate that the light-induced anthocyanin biosynthesis regulatory mechanism in pear differs from that described in previous reports suggesting that a bZIP family member co-regulates anthocyanin biosynthesis with other transcription factors in apple and Arabidopsis.It was found that,in response to light,PybZIPa promoted anthocyanin biosynthesis by regulating important transcription factors(PyMYB114,PyMYB10,and PyBBX22)as well as structural genes(PyUFGT)via binding to G-boxes within promoters.This activation was amplified by the self-binding of PybZIPa to activate its own promoter.Overall,we demonstrate the utility of a multiomics integrative approach for discovering new functional genes and pathways underlying light-induced anthocyanin biosynthesis.
文摘The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be radiologically activated prior to treatment and“coerce”tumor cells into readily absorb these nanoparticles.This targeted intracytoplasmic delivery of short-lived radiologically activated nanoparticles could provide less“whole-body”radiation dose while delivering a short lived potent tumor localized dose,along with their low toxicity may prove to be another tool in the treatment of diverse cancers.
