目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭...目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭代优化,直至收敛。结果:采用此方法分辨所得光谱还原率高,定量结果的浓度值与真实值之间线性关系良好。结论:本方法用于药物色谱重叠峰分辨结果可靠。展开更多
Nanotheranostics with comprehensive diagnostic and therapeutic capabilities show exciting cancer treatment potentials.Here,we develop an excipient-free drug delivery system for cancer diagnosis as well as therapy,in w...Nanotheranostics with comprehensive diagnostic and therapeutic capabilities show exciting cancer treatment potentials.Here,we develop an excipient-free drug delivery system for cancer diagnosis as well as therapy,in which a near infra-red photosensitizer and a chemotherapeutic drug can be self-delivered without any carriers.The building block of the drug delivery system was synthesized by covalently conjugating four anticancer drugs(7-ethyl-10-hydroxy-camptothecin,SN-38)with a photosensitizer(porphyrin)via hydrolyzable ester linkage,which endows the drug delivery system with 100%active pharmaceutical ingredients,excellent imaging,and therapeutic functionalities.The conjugates can readily self-assemble into nanosheets(PS NSs)and remain stable for at least 20 days in aqueous solution.In PS NSs,fluorescence resonance energy transfer(FRET)dominates the fluorescence of SN-38 and enables to monitor the drug release fluorescentiy.The PS NSs also show excellent anticancer activity in vitro,due to the increased cell uptake with the synergistic effect of photodynamic therapy and chemotherapy.展开更多
基金Supported by Sino-French Advanced Research Project(PRA B02-07)Scientific and technological achievements promotion project of Guangzhou city(2006C13G0011)
文摘目的:应用多元曲线分辨-交替最小二乘(MCR-ALS)法,对7-乙基-10-羟基喜树碱与喜树碱的色谱重叠峰进行分辨,并对杂质喜树碱进行定量。方法:以渐进因子分析(EFA)解析结果作为初始值对喜树碱与7-乙基-10-羟基喜树碱的色谱重叠峰进行 ALS 迭代优化,直至收敛。结果:采用此方法分辨所得光谱还原率高,定量结果的浓度值与真实值之间线性关系良好。结论:本方法用于药物色谱重叠峰分辨结果可靠。
基金The authors gratefully acknowledge the support from Dr.Lis faculty startup funds at UC Davis and Dr.Xues National Natural Science Foundation of China(NSFC)(No.81803002).
文摘Nanotheranostics with comprehensive diagnostic and therapeutic capabilities show exciting cancer treatment potentials.Here,we develop an excipient-free drug delivery system for cancer diagnosis as well as therapy,in which a near infra-red photosensitizer and a chemotherapeutic drug can be self-delivered without any carriers.The building block of the drug delivery system was synthesized by covalently conjugating four anticancer drugs(7-ethyl-10-hydroxy-camptothecin,SN-38)with a photosensitizer(porphyrin)via hydrolyzable ester linkage,which endows the drug delivery system with 100%active pharmaceutical ingredients,excellent imaging,and therapeutic functionalities.The conjugates can readily self-assemble into nanosheets(PS NSs)and remain stable for at least 20 days in aqueous solution.In PS NSs,fluorescence resonance energy transfer(FRET)dominates the fluorescence of SN-38 and enables to monitor the drug release fluorescentiy.The PS NSs also show excellent anticancer activity in vitro,due to the increased cell uptake with the synergistic effect of photodynamic therapy and chemotherapy.
