目的观察益气活血方对急性脑缺血再灌注大鼠缺血脑组织谷氨酸(glutamate,Glu)、γ-氨基丁酸(γ-aminobutyrate acid,GABA)含量及神经受体1(neuroreceptor1,NR1)表达的影响。方法采用线栓法阻断大鼠左侧大脑中动脉复制局灶性脑缺血再灌...目的观察益气活血方对急性脑缺血再灌注大鼠缺血脑组织谷氨酸(glutamate,Glu)、γ-氨基丁酸(γ-aminobutyrate acid,GABA)含量及神经受体1(neuroreceptor1,NR1)表达的影响。方法采用线栓法阻断大鼠左侧大脑中动脉复制局灶性脑缺血再灌注模型。大鼠随机分为假手术组、模型组、益气活血汤组。采用高效液相色谱法和链霉亲合素-生物素酶复合物(streptavidin-biotinidase complex,SABC)染色法,分别检测Glu、GABA含量和NR1表达水平。结果脑缺血2 h再灌注各时间点与假手术组比较,模型组Glu、GABA含量和NR1表达均显著升高(P<0.05,P<0.01);与模型组比较,治疗组脑缺血后6,48,72 h Glu、NR1水平显著降低;72 h GABA含量显著提高(P<0.01)。结论益气活血方可能通过调节兴奋性氨基酸Glu和抑制性氨基酸GABA含量的平衡,及减少NR1表达防止脑缺血再灌注损伤。展开更多
Melatonin and gamma-aminobutyric acid（GABA） have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective si...Melatonin and gamma-aminobutyric acid（GABA） have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective single-center non-randomized controlled clinical trial performed in the China Rehabilitation Research Center, we analyzed the relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke. Patients during rehabilitation of stroke were recruited and assigned to the insomnia group or non-insomnia group. Simultaneously, persons without stroke or insomnia served as normal controls. Each group contained 25 cases. The primary outcome was nocturnal concentrations of melatonin, GABA and total antioxidants in peripheral blood. The secondary outcomes were Pittsburgh Sleep Quality Index, Insomnia Severity Index, Epworth Sleepiness Scale, Fatigue Severity Scale, Morningness-Eveningness Questionnaire（Chinese version）, and National Institute of Health Stroke Scale. The relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke was analyzed and showed that they were lower in the insomnia group than in the non-insomnia group. The severity of stroke was higher in the insomnia group than in the non-insomnia group. Correlation analysis demonstrated that the nocturnal concentrations of melatonin and GABA were associated with insomnia after stroke. This trial was registered at Clinical Trials.gov, identifier： NCT03202121.展开更多
文摘目的观察益气活血方对急性脑缺血再灌注大鼠缺血脑组织谷氨酸(glutamate,Glu)、γ-氨基丁酸(γ-aminobutyrate acid,GABA)含量及神经受体1(neuroreceptor1,NR1)表达的影响。方法采用线栓法阻断大鼠左侧大脑中动脉复制局灶性脑缺血再灌注模型。大鼠随机分为假手术组、模型组、益气活血汤组。采用高效液相色谱法和链霉亲合素-生物素酶复合物(streptavidin-biotinidase complex,SABC)染色法,分别检测Glu、GABA含量和NR1表达水平。结果脑缺血2 h再灌注各时间点与假手术组比较,模型组Glu、GABA含量和NR1表达均显著升高(P<0.05,P<0.01);与模型组比较,治疗组脑缺血后6,48,72 h Glu、NR1水平显著降低;72 h GABA含量显著提高(P<0.01)。结论益气活血方可能通过调节兴奋性氨基酸Glu和抑制性氨基酸GABA含量的平衡,及减少NR1表达防止脑缺血再灌注损伤。
基金supported by the Optional Research Project of China Rehabilitation Research Center,No.2014-7the Sub-Project under National“Twelfth Five-Year”Plan for Science&Technology Support Project,No.2011BAI08B11
文摘Melatonin and gamma-aminobutyric acid（GABA） have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective single-center non-randomized controlled clinical trial performed in the China Rehabilitation Research Center, we analyzed the relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke. Patients during rehabilitation of stroke were recruited and assigned to the insomnia group or non-insomnia group. Simultaneously, persons without stroke or insomnia served as normal controls. Each group contained 25 cases. The primary outcome was nocturnal concentrations of melatonin, GABA and total antioxidants in peripheral blood. The secondary outcomes were Pittsburgh Sleep Quality Index, Insomnia Severity Index, Epworth Sleepiness Scale, Fatigue Severity Scale, Morningness-Eveningness Questionnaire（Chinese version）, and National Institute of Health Stroke Scale. The relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke was analyzed and showed that they were lower in the insomnia group than in the non-insomnia group. The severity of stroke was higher in the insomnia group than in the non-insomnia group. Correlation analysis demonstrated that the nocturnal concentrations of melatonin and GABA were associated with insomnia after stroke. This trial was registered at Clinical Trials.gov, identifier： NCT03202121.
