Brown adipose tissue (BAT) plays an essential role in non-shivering thermogenesis. The phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) is identified as a lipid transporter that reciprocally transfers ph...Brown adipose tissue (BAT) plays an essential role in non-shivering thermogenesis. The phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) is identified as a lipid transporter that reciprocally transfers phospholipids between intracellular membrane structures. However, the physiological significance of PITPNC1 and its regulatory mechanism remain unclear. Here, we demonstrate that PITPNC1 is a key player in thermogenesis of BAT. While Pitpnc1^(−/−) mice do not differ with wildtype mice in body weight and insulin sensitivity on either chow or high-fat diet, they develop hypothermia when subjected to acute cold exposure at 4℃. The Pitpnc1^(−/−) brown adipocytes exhibit defective β-oxidation and abnormal thermogenesis-related metabolism pathways in mitochondria. The deficiency of lipid mobilization in Pitpnc1^(−/−) brown adipocytes might be the result of excessive accumulation of phosphatidylcholine and a reduction of phosphatidic acid. Our findings have uncovered significant roles of PITPNC1 in mitochondrial phospholipid homeostasis and BAT thermogenesis.展开更多
Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （...Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its t-oxidation. Cultured in the presence of 5-phenylvaleric acid （PVA）, the edited P. entomophila produced only homopolymer poly（3-hydroxy-5-phenylvalerate） or P（3HPhV）. While copolyesters P（3HPhV-co-3HDD） of 3-hydroxy-5-phenylvalerate （3HPhV） and 3-hydroxydodecanoate （3HDD） were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid （DDA）. Compositions of 3HPhV in P（3HPhV-co-3HDD） were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance （NMR） spectra clearly indicated that the polymers were homopolymer of P（3HPhV） and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically de- pending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.展开更多
2,4-dienoyl CoA reductase gene （DECR 1） is mapped on pig 4 q1.2, includes ten exons and nine introns of variable size that span 30 kb. DECR 1 gene participates in the β-oxidation pathway, affects the content of int...2,4-dienoyl CoA reductase gene （DECR 1） is mapped on pig 4 q1.2, includes ten exons and nine introns of variable size that span 30 kb. DECR 1 gene participates in the β-oxidation pathway, affects the content of intramuscular fatty acid, especially the percentage of linoleic acid. The expression of DECR 1 gene has important influence on IMF, the pH, and the meat colour of pork further affects the meat quality.展开更多
基金the National Key R&D Program of China(2018YFA0506900)the National Key R&D Program of China(2018YFA0800301)+3 种基金the National Natural Science Foundation of China(91857103)Shanghai Basic Research Field Project“Science and Technology Innovation Action Plan”(21JC1400400)the Lingang Laboratory(LG-QS-202204-06)Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)。
文摘Brown adipose tissue (BAT) plays an essential role in non-shivering thermogenesis. The phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) is identified as a lipid transporter that reciprocally transfers phospholipids between intracellular membrane structures. However, the physiological significance of PITPNC1 and its regulatory mechanism remain unclear. Here, we demonstrate that PITPNC1 is a key player in thermogenesis of BAT. While Pitpnc1^(−/−) mice do not differ with wildtype mice in body weight and insulin sensitivity on either chow or high-fat diet, they develop hypothermia when subjected to acute cold exposure at 4℃. The Pitpnc1^(−/−) brown adipocytes exhibit defective β-oxidation and abnormal thermogenesis-related metabolism pathways in mitochondria. The deficiency of lipid mobilization in Pitpnc1^(−/−) brown adipocytes might be the result of excessive accumulation of phosphatidylcholine and a reduction of phosphatidic acid. Our findings have uncovered significant roles of PITPNC1 in mitochondrial phospholipid homeostasis and BAT thermogenesis.
基金supported by the National High Technology Research and Development Program of China(2012AA023102 to Liu Lei,Guo Kai and Wu Qiong)the National Basic Research Program of China(2012CB725201 to Chen GuoQiang and Chen JinChun,2012CB725204 to Guo Kai and Wu Qiong)National Natural Science Foundation of China(31270146 to Chen GuoQiang)
文摘Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its t-oxidation. Cultured in the presence of 5-phenylvaleric acid （PVA）, the edited P. entomophila produced only homopolymer poly（3-hydroxy-5-phenylvalerate） or P（3HPhV）. While copolyesters P（3HPhV-co-3HDD） of 3-hydroxy-5-phenylvalerate （3HPhV） and 3-hydroxydodecanoate （3HDD） were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid （DDA）. Compositions of 3HPhV in P（3HPhV-co-3HDD） were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance （NMR） spectra clearly indicated that the polymers were homopolymer of P（3HPhV） and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically de- pending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.
基金Supported by Heilongjiang Province Tackle Key Problem Item(GB01B104)
文摘2,4-dienoyl CoA reductase gene （DECR 1） is mapped on pig 4 q1.2, includes ten exons and nine introns of variable size that span 30 kb. DECR 1 gene participates in the β-oxidation pathway, affects the content of intramuscular fatty acid, especially the percentage of linoleic acid. The expression of DECR 1 gene has important influence on IMF, the pH, and the meat colour of pork further affects the meat quality.
