[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sod...[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.展开更多
用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花...用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花卉。产于云南、广西西部,其花期长,观赏价值高。多年来均用种子繁殖,速度较慢。如改用组织培养法,可大大提高其生产效率。但其组织培养工作在国内外尚未见报道,为此,我们对其进行了愈伤组织的诱导和植株再生的研究,得到了初步结果。展开更多
基金Supported by the National Natural Science Foundation(30670177)Scientific Research Foundation for the Outstanding Young Scientist of Shangdong Province(006BS06002)National Ministry of Education Doctoral Fund(20050445003)~~
文摘[Objective] This study aimed to investigate the salt-tolerance mechanism of sweet potato.[Method]Two sweet potato varieties of Xu 25-2(salt-tolerant cultivar)and Triumph 100(salt-sensitive cultivar)were treated by sodium chloride with the concentration of 0 mmol/L and 100 mmol/L.After 20 days,Na+ content and Na+/K+ ratio in the roots,shoots and leave were determined by the flame photometer,while dry weight and fresh weight of roots,shoots and leave in different varieties were also studied.[Result]The growth of two sweet potato varieties was inhibited under salt stress,so the plant became shorter,leaf and root became fewer,dry weight of roots and leave decreased,but seedlings of Xu 25-2 were inhibited slightly.Furthermore,Na+ content and Na+/K+ ratio in roots,shoots and leaves of two sweet potato varieties increased.Na+ content of salt-tolerant Xu 25-2 was low in roots,shoots and leaves,while Na+ content of salt-sensitive Triumph 100 was high in shoots and leave of seedlings,but the change range of Xu 25-2 was less than that of Triumph 100.[Conclusion]The lower Na+ content and Na+/K+ ratio in leaves under salt stress were the most important characteristics for salt-tolerance of sweet potato varieties.
文摘用报春花叶片、花蕾作外植体,接种在含有不同浓度6BA、NAA 的 MS 培养基上,诱导愈伤组织形成,经愈伤组织增殖和反复切割后诱导其分化出芽和根,然后将试管苗移植到土壤中。报春花(Primula malacoides),是报春花科报春花属的二年生草本花卉。产于云南、广西西部,其花期长,观赏价值高。多年来均用种子繁殖,速度较慢。如改用组织培养法,可大大提高其生产效率。但其组织培养工作在国内外尚未见报道,为此,我们对其进行了愈伤组织的诱导和植株再生的研究,得到了初步结果。