BACKGROUND： Pharmacological research has shown that cactus polysaccharide （CP） has anti-oxidant, anti-inflammatory, anti-tumor, anti-aging, and immune-stimulating activities. It may also provide protective effects ...BACKGROUND： Pharmacological research has shown that cactus polysaccharide （CP） has anti-oxidant, anti-inflammatory, anti-tumor, anti-aging, and immune-stimulating activities. It may also provide protective effects against oxidative stress injuries in the rat brain. OBJECTIVE： To validate the effects of CP on H2O2-induced oxidative stress injuries in the rat cerebral cortex and hippocampal slices 30 minutes prior to injury, as well as 30 minutes and 2.5 hours after injury. DESIGN： A randomized controlled experiment. SETTINGS： Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology; Department of Pharmacology, College of Medical Science, Yangtze University. MATERIALS： A total of 50 male Sprague Dawley （SD） rats, normal grade and weighing 200 300 g, were provided by the Laboratory Animal Center of Tongji Medical College, Huazhong University of Science and Technology. The protocol was performed in accordance with ethical guidelines for the use and care of animals. Cactus polysaccharide, a dried needle crystal, was extracted from Opuntia milpa alta at the Chemistry and Environment Engineering School of Yangtze University. The following chemicals and instruments were used： 2,3,5-triphenyl tetrazolium chloride （Sigma, St Louis, Missouri, USA）; lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, glutathione （GSH）, and total antioxidant competence （T-AOC） assays （Jiancheng-Bioeng Institute, Nanjing）; McIllwain tissue chopper （Mickle Laboratory Engineering, USA）; and ELISA reader and Magellan software （TECAN, Austria）. METHODS： This experiment was performed at the Department of Pharmacology, Medical College of Yangtze University, between March and June 2006. All rats were sacrificed after anesthesia. The cerebral cortex and hippocampus were dissected. Several cerebral cortex and hippocampus slices were selected as controls, while other sections were co-incubated with H2O2 for 30 minutes to induce an oxidative stress injury.展开更多
To study the effects of6 - hydroxydopamine(6 - OHDA) and reduced glutathione(GSH) on the nigral dopaminergic neurons in brain slices in vitro,immolunohistochem ical technique was used to observe the changes of TH - ...To study the effects of6 - hydroxydopamine(6 - OHDA) and reduced glutathione(GSH) on the nigral dopaminergic neurons in brain slices in vitro,immolunohistochem ical technique was used to observe the changes of TH - stained neurons,including cell bodies and the dendrites,in the substantia nigra (SN) of midbrain slices of rats after incubation for 1h in the presence of GSH 15 m in before and during the period of incubation with6 - OHDA.The results showed thatcell bodies rem ained intact but dendrites were fragm ented and truncated after treatment with6 - OHDA.The antioxidant GSH alone did not significantly affect the dendrites of SN neurons but prevented6 - O- HDA- induced damage of dendrites.It was concluded that glutathione m ay prevent6 - OHDA- in- duced dopam inergic neurodegeneration and play a protective role in dopaminergic neurons.展开更多
基金the Science Foundation of Hubei Provincial Department of Education, No. Q200712004
文摘BACKGROUND： Pharmacological research has shown that cactus polysaccharide （CP） has anti-oxidant, anti-inflammatory, anti-tumor, anti-aging, and immune-stimulating activities. It may also provide protective effects against oxidative stress injuries in the rat brain. OBJECTIVE： To validate the effects of CP on H2O2-induced oxidative stress injuries in the rat cerebral cortex and hippocampal slices 30 minutes prior to injury, as well as 30 minutes and 2.5 hours after injury. DESIGN： A randomized controlled experiment. SETTINGS： Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology; Department of Pharmacology, College of Medical Science, Yangtze University. MATERIALS： A total of 50 male Sprague Dawley （SD） rats, normal grade and weighing 200 300 g, were provided by the Laboratory Animal Center of Tongji Medical College, Huazhong University of Science and Technology. The protocol was performed in accordance with ethical guidelines for the use and care of animals. Cactus polysaccharide, a dried needle crystal, was extracted from Opuntia milpa alta at the Chemistry and Environment Engineering School of Yangtze University. The following chemicals and instruments were used： 2,3,5-triphenyl tetrazolium chloride （Sigma, St Louis, Missouri, USA）; lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, glutathione （GSH）, and total antioxidant competence （T-AOC） assays （Jiancheng-Bioeng Institute, Nanjing）; McIllwain tissue chopper （Mickle Laboratory Engineering, USA）; and ELISA reader and Magellan software （TECAN, Austria）. METHODS： This experiment was performed at the Department of Pharmacology, Medical College of Yangtze University, between March and June 2006. All rats were sacrificed after anesthesia. The cerebral cortex and hippocampus were dissected. Several cerebral cortex and hippocampus slices were selected as controls, while other sections were co-incubated with H2O2 for 30 minutes to induce an oxidative stress injury.
基金This project was supported by a grant from Natural Sci-ences Foundation of China(No. 30 170 334)
文摘To study the effects of6 - hydroxydopamine(6 - OHDA) and reduced glutathione(GSH) on the nigral dopaminergic neurons in brain slices in vitro,immolunohistochem ical technique was used to observe the changes of TH - stained neurons,including cell bodies and the dendrites,in the substantia nigra (SN) of midbrain slices of rats after incubation for 1h in the presence of GSH 15 m in before and during the period of incubation with6 - OHDA.The results showed thatcell bodies rem ained intact but dendrites were fragm ented and truncated after treatment with6 - OHDA.The antioxidant GSH alone did not significantly affect the dendrites of SN neurons but prevented6 - O- HDA- induced damage of dendrites.It was concluded that glutathione m ay prevent6 - OHDA- in- duced dopam inergic neurodegeneration and play a protective role in dopaminergic neurons.
