For realizing non-contact steering swimming of a capsule robot in curved environment filled with viscous liquid, based on spa- tial orthogonal superposition theorem of alternating magnetic vectors, an innovative physi...For realizing non-contact steering swimming of a capsule robot in curved environment filled with viscous liquid, based on spa- tial orthogonal superposition theorem of alternating magnetic vectors, an innovative physical method is proposed, which em- ploys three-axis orthogonal square Helmholtz coils fed with three phase sine currents to create a universal uniform magnetic spin vector as energy source. According to the antiphase sine current superposition theorem generalized in this paper, an effec- tive control method for successively adjusting the orientation and the rotating direction of the universal magnetic spin vector is proposed. For validating its feasibility and controllability, three-axis Helmholtz coils, power source and an innovative capsule robot prototype were manufactured, experiments were conducted in both spiral pipe and animal intestine. It was demonstrated that the orientation and the rotational direction of the universal uniform-magnetic spin vector can be adjusted successively through digital control and steering swimming of the capsule robot in spiral intestine can be achieved successfully. The breakthrough of the universal rotating uniform-magnetic vector will push forward the development of modern physics and biomedical engineering展开更多
Signal peptide capable of efficiently directing many protein secretion in mammalian cells is one of the key elements in recombinant protein production,gene therapy and the development of DNA vaccines.In order to explo...Signal peptide capable of efficiently directing many protein secretion in mammalian cells is one of the key elements in recombinant protein production,gene therapy and the development of DNA vaccines.In order to explore the possibility of rat growth hormone signal peptide as such an element,a new vector based on the mammalian expression vector pcDNA3 was constructed by employing rat growth hormone(rGH) signal peptide as leading sequence,followed by multiple cloning sites,the myc epitope-tag and 6×his purification tag in the expression cassette.The vector was validated by successfully expressing and secretion of chick MMP-2 C-terminal PEX domain,a potential angiogenesis inhibitor,and tandem peptide repeats of myc epitope-tag in COS-7 cells.These results suggest that rat growth hormone signal peptide is effective in the mediation of recombinant protein expression and secretion,and this vector provides a new tool for universal cloning and secretion of exogenous proteins in mammalian cells.展开更多
We discuss the properties of complex manifolds having rational homology of S 1 × S 2n?1 including those constructed by Hopf, Kodaira and Brieskorn-van de Ven. We extend certain previously known properties of coho...We discuss the properties of complex manifolds having rational homology of S 1 × S 2n?1 including those constructed by Hopf, Kodaira and Brieskorn-van de Ven. We extend certain previously known properties of cohomology of bundles on such manifolds. As an application we consider degeneration of Hodge-de Rham spectral sequence in this non Kahler setting.展开更多
以质粒 pc DNA3和 p SV2 -dhfr为基础 ,构建了 2个通用表达载体 p MCD-A和 p MCD-B,然后将 EPOminigene克隆于它们的多克隆位点之中 ,得到表达载体 p AE和 p BE,经脂质体导入 CHO-dhfr- 细胞瞬时表达 ,结果表明 ,p MCD-A和 p MCD-B表达...以质粒 pc DNA3和 p SV2 -dhfr为基础 ,构建了 2个通用表达载体 p MCD-A和 p MCD-B,然后将 EPOminigene克隆于它们的多克隆位点之中 ,得到表达载体 p AE和 p BE,经脂质体导入 CHO-dhfr- 细胞瞬时表达 ,结果表明 ,p MCD-A和 p MCD-B表达效果较好 ,表达产物经网织红细胞法测定 。展开更多
[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of ex...[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of existing pGreen0229 plant expression vector, phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed, which could be used to connect target gene of maize expression vector PGM-35Sbar, and transform Ji444 maize inbred lines by pollen tube pathway. [Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed. When the maize plants were transformed, 14 resistant plants were obtained, and 12 plants were identified to be positive plants by PCR detection. [Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene.展开更多
对单十字轴万向节的十字轴运动规律进行了分析,运用数学向量方法求得十字轴万向节的传递运动规律,以及十字轴平面法线运动轨迹方程;运用Solid Works Motion软件来仿真单十字轴万向节中十字轴平面法线的运动轨迹,对比仿真所得的波动图与...对单十字轴万向节的十字轴运动规律进行了分析,运用数学向量方法求得十字轴万向节的传递运动规律,以及十字轴平面法线运动轨迹方程;运用Solid Works Motion软件来仿真单十字轴万向节中十字轴平面法线的运动轨迹,对比仿真所得的波动图与理论计算所得的方程,验证了理论分析结果的正确性,为单十字轴万向节的运动优化设计提供理论依据,同时在空间上,为单十字轴万向节转向系的空间布置提供理论参考。展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 60875064,61175102,and 51277018)
文摘For realizing non-contact steering swimming of a capsule robot in curved environment filled with viscous liquid, based on spa- tial orthogonal superposition theorem of alternating magnetic vectors, an innovative physical method is proposed, which em- ploys three-axis orthogonal square Helmholtz coils fed with three phase sine currents to create a universal uniform magnetic spin vector as energy source. According to the antiphase sine current superposition theorem generalized in this paper, an effec- tive control method for successively adjusting the orientation and the rotating direction of the universal magnetic spin vector is proposed. For validating its feasibility and controllability, three-axis Helmholtz coils, power source and an innovative capsule robot prototype were manufactured, experiments were conducted in both spiral pipe and animal intestine. It was demonstrated that the orientation and the rotational direction of the universal uniform-magnetic spin vector can be adjusted successively through digital control and steering swimming of the capsule robot in spiral intestine can be achieved successfully. The breakthrough of the universal rotating uniform-magnetic vector will push forward the development of modern physics and biomedical engineering
文摘Signal peptide capable of efficiently directing many protein secretion in mammalian cells is one of the key elements in recombinant protein production,gene therapy and the development of DNA vaccines.In order to explore the possibility of rat growth hormone signal peptide as such an element,a new vector based on the mammalian expression vector pcDNA3 was constructed by employing rat growth hormone(rGH) signal peptide as leading sequence,followed by multiple cloning sites,the myc epitope-tag and 6×his purification tag in the expression cassette.The vector was validated by successfully expressing and secretion of chick MMP-2 C-terminal PEX domain,a potential angiogenesis inhibitor,and tandem peptide repeats of myc epitope-tag in COS-7 cells.These results suggest that rat growth hormone signal peptide is effective in the mediation of recombinant protein expression and secretion,and this vector provides a new tool for universal cloning and secretion of exogenous proteins in mammalian cells.
文摘We discuss the properties of complex manifolds having rational homology of S 1 × S 2n?1 including those constructed by Hopf, Kodaira and Brieskorn-van de Ven. We extend certain previously known properties of cohomology of bundles on such manifolds. As an application we consider degeneration of Hodge-de Rham spectral sequence in this non Kahler setting.
文摘以质粒 pc DNA3和 p SV2 -dhfr为基础 ,构建了 2个通用表达载体 p MCD-A和 p MCD-B,然后将 EPOminigene克隆于它们的多克隆位点之中 ,得到表达载体 p AE和 p BE,经脂质体导入 CHO-dhfr- 细胞瞬时表达 ,结果表明 ,p MCD-A和 p MCD-B表达效果较好 ,表达产物经网织红细胞法测定 。
基金Supported by National Transgenic Special Project(2009ZX08003-009B)Beijing Science and Technology Commission Project(KJCX201102003)~~
文摘[Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. [Method] Based on the transformation of existing pGreen0229 plant expression vector, phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed, which could be used to connect target gene of maize expression vector PGM-35Sbar, and transform Ji444 maize inbred lines by pollen tube pathway. [Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed. When the maize plants were transformed, 14 resistant plants were obtained, and 12 plants were identified to be positive plants by PCR detection. [Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene.
文摘对单十字轴万向节的十字轴运动规律进行了分析,运用数学向量方法求得十字轴万向节的传递运动规律,以及十字轴平面法线运动轨迹方程;运用Solid Works Motion软件来仿真单十字轴万向节中十字轴平面法线的运动轨迹,对比仿真所得的波动图与理论计算所得的方程,验证了理论分析结果的正确性,为单十字轴万向节的运动优化设计提供理论依据,同时在空间上,为单十字轴万向节转向系的空间布置提供理论参考。
