[目的]利用网络药理学分析调经汤与克罗米芬治疗卵巢早衰(premature ovarian failure,POF)的机制差异,探讨调经汤治疗POF的可能机制。[方法]利用京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)DRUG数据库、...[目的]利用网络药理学分析调经汤与克罗米芬治疗卵巢早衰(premature ovarian failure,POF)的机制差异,探讨调经汤治疗POF的可能机制。[方法]利用京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)DRUG数据库、中药系统药理学数据库与分析平台(Traditional Chinese Medicine Database and Analysis Platform,TCMSP)挖掘克罗米芬的分子结构及调经汤潜在活性成分与药物靶点,通过人类基因数据库(Human Gene Database,GeneCards)、在线人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)、药物基因组学知识库(Pharmacogenomics Knowledgebase,PharmGKB),筛选POF的相关疾病靶点。将得到的药物靶点与疾病靶点进行匹配,得到药物-疾病交集靶点数据库,利用Cytoscape 3.8.0软件构建中药成分-靶点-疾病网络。使用STRING网站对得到的交集靶点进行蛋白互作分析,利用R语言进行基因本体(gene oncology,GO)分析和KEGG富集分析。[结果]克罗米芬的靶蛋白为雌激素受体,KEGG通路为雌激素信号通路。调经汤中共筛选出181个活性成分和2873个潜在靶点,POF靶点4597个。通过比对调经汤和POF的共同靶点,筛选出192个关键基因。这些共同靶点涉及的生物学过程主要是氧化物及氧化应激的反应、细胞对化学物应激刺激的反应和物质代谢反应等,发挥转录、结合等分子功能;生物学通路包括白细胞介素-17(interleukin-17,IL-17)、肿瘤坏死因子(tumor necrosis factor,TNF)、磷脂酰肌醇3激酶-蛋白激酶B(phosphatidylinositol 3 kinaseprotein kinase B,PI3K-AKT)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、叉头框转录因子O亚族(forkhead box transcription factor O,FoxO)、雌激素、Wnt信号通路等。[结论]调经汤与克罗米芬的共有通路为雌激素信号通路,但与克罗米芬相比,调经汤可通过多个靶点、多条通路起到治疗POF的作用。展开更多
Objective: To observe the effect of tiaojing decoction and climen on diminished ovarian reserve patients with de ficiency of Kidney-Yin by sex hormone and uterine artery blood flow parameters. To verify the e ffective...Objective: To observe the effect of tiaojing decoction and climen on diminished ovarian reserve patients with de ficiency of Kidney-Yin by sex hormone and uterine artery blood flow parameters. To verify the e ffectiveness of tiaojing decoction on diminished ovarian reserve and uterine artery blood flow parameters, explore the correlation between uterine artery blood flow parameters and sex hormones. Methods: Total 60 diminished ovarian reserve patients with deficiency of Kidney-Yin were randomly divided into the experimental group (n=30, taking tiaojing decoction and climen) and the control group (n=30, taking climen) and they were treated in 3 months. Observe bFSH, bE2, uterine artery PSV, EDV, S/D, RI before and after the treatment in the 2-4 day of menstruation. Results: After treatment, serum sex hormone levels and uterine artery blood flow parameters of the two groups were significantly different (P < 0.05), and the experimental group was significantly better than the control group. There was a linear correlation between PSV and FSH, PSV and E2, FSH and E2. Conclusion: Tiaojing decoction combined with climen has significant effect on diminished ovarian reserve patients with deficiency of Kidney-Yin. Uterine artery blood flow parameters can reflect the sex hormone levels.展开更多
文摘[目的]利用网络药理学分析调经汤与克罗米芬治疗卵巢早衰(premature ovarian failure,POF)的机制差异,探讨调经汤治疗POF的可能机制。[方法]利用京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)DRUG数据库、中药系统药理学数据库与分析平台(Traditional Chinese Medicine Database and Analysis Platform,TCMSP)挖掘克罗米芬的分子结构及调经汤潜在活性成分与药物靶点,通过人类基因数据库(Human Gene Database,GeneCards)、在线人类孟德尔遗传数据库(Online Mendelian Inheritance in Man,OMIM)、药物基因组学知识库(Pharmacogenomics Knowledgebase,PharmGKB),筛选POF的相关疾病靶点。将得到的药物靶点与疾病靶点进行匹配,得到药物-疾病交集靶点数据库,利用Cytoscape 3.8.0软件构建中药成分-靶点-疾病网络。使用STRING网站对得到的交集靶点进行蛋白互作分析,利用R语言进行基因本体(gene oncology,GO)分析和KEGG富集分析。[结果]克罗米芬的靶蛋白为雌激素受体,KEGG通路为雌激素信号通路。调经汤中共筛选出181个活性成分和2873个潜在靶点,POF靶点4597个。通过比对调经汤和POF的共同靶点,筛选出192个关键基因。这些共同靶点涉及的生物学过程主要是氧化物及氧化应激的反应、细胞对化学物应激刺激的反应和物质代谢反应等,发挥转录、结合等分子功能;生物学通路包括白细胞介素-17(interleukin-17,IL-17)、肿瘤坏死因子(tumor necrosis factor,TNF)、磷脂酰肌醇3激酶-蛋白激酶B(phosphatidylinositol 3 kinaseprotein kinase B,PI3K-AKT)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、叉头框转录因子O亚族(forkhead box transcription factor O,FoxO)、雌激素、Wnt信号通路等。[结论]调经汤与克罗米芬的共有通路为雌激素信号通路,但与克罗米芬相比,调经汤可通过多个靶点、多条通路起到治疗POF的作用。
文摘Objective: To observe the effect of tiaojing decoction and climen on diminished ovarian reserve patients with de ficiency of Kidney-Yin by sex hormone and uterine artery blood flow parameters. To verify the e ffectiveness of tiaojing decoction on diminished ovarian reserve and uterine artery blood flow parameters, explore the correlation between uterine artery blood flow parameters and sex hormones. Methods: Total 60 diminished ovarian reserve patients with deficiency of Kidney-Yin were randomly divided into the experimental group (n=30, taking tiaojing decoction and climen) and the control group (n=30, taking climen) and they were treated in 3 months. Observe bFSH, bE2, uterine artery PSV, EDV, S/D, RI before and after the treatment in the 2-4 day of menstruation. Results: After treatment, serum sex hormone levels and uterine artery blood flow parameters of the two groups were significantly different (P < 0.05), and the experimental group was significantly better than the control group. There was a linear correlation between PSV and FSH, PSV and E2, FSH and E2. Conclusion: Tiaojing decoction combined with climen has significant effect on diminished ovarian reserve patients with deficiency of Kidney-Yin. Uterine artery blood flow parameters can reflect the sex hormone levels.
