Quantification of soil spatial and temporal variability at watershed scale is important in ecological modeling, precision agriculture, and natural resources management. The spatio-temporal variations of soil nitrogen ...Quantification of soil spatial and temporal variability at watershed scale is important in ecological modeling, precision agriculture, and natural resources management. The spatio-temporal variations of soil nitrogen under different land uses in a small watershed （12.10 km^2） in the hilly area of purple soil at the upper reaches of the Yangtze River in southwestern China were investigated by using conventional statistics, geostatistics, and a geographical information system in order to provide information for land management and control of environmental issues. A total of 552 soil samples （o to 15 cm） from 276 sites within the watershed were collected in April and August of 2o11, and analyzed for soil total nitrogen （STN） and nitrate nitrogen （NO3-N）. We compared spatial variations of STN and NO3-N under different land uses as well as the temporal variations in April （dry season） and August （rainy season）. Results showed that STN contents were deeply affected by land-use types; median STN values ranged from 0.94to 1.27g.kg-I, and STN contents decreased in the following order： paddy field 〉 foresfland 〉 sloping cropland. No significant difference was found for STN contents between April and August under the same land use. However, NO3- N contents were 23.26, 10.58, and 26.19 mg·kg^-1 in April, and 1.34, 8.51, and 3.00 mg·kg^-1 in August for the paddy field, sloping cropland and forestland, respectively. Nugget ratios for STN indicatedmoderate spatial dependence in the paddy field and sloping cropland, and a strong spatial dependence in forestland. The processes of nitrogen movement, transformation, absorption of plant were deeply influenced by land use types; as a result, great changes of soil nitrogen levels at spatial and temporal scales were demonstrated in the studied watershed.展开更多
AIM:To establish the more feasible and sensitive assessment approach to the detection of adefovir (ADV) resistance-associated hepatitis B virus (HBV) quasispecies.METHODS: Based on the characteristics of rtA181V/T and...AIM:To establish the more feasible and sensitive assessment approach to the detection of adefovir (ADV) resistance-associated hepatitis B virus (HBV) quasispecies.METHODS: Based on the characteristics of rtA181V/T and rtN236T mutations, a new approach based on real-time fluorescent quantitative polymerase chain reaction (RT-PCR) was established for the detection of ADV-resistant HBV quasispecies, total HBV DNA, rtA181 and rtN236 mutations in blood samples from 32 chronic hepatitis B (CHB) patients with unsatisfactory curative effect on ADV and compared with routine HBV DNA sequencing.RESULTS: Both the sensitivity and specificity of this new detection approach to ADV-resistant HBV quasispecies were 100%, which were much higher than those of direct HBV DNA sequencing. The approach was able to detect 0.1% of mutated strains in a total plasmid population. Among the 32 clinical patients, single rtA181 and rtN236T mutation and double rtA181T and rtN236T mutations were detected in 20 and 8, respectively, while ADV-resistant mutations in 6 (including, rtA181V/T mutation alone in 5 patients) and no associated mutations in 26.CONCLUSION: This new approach is more feasible and efficient to detect ADV-resistant mutants of HBV and ADV-resistant mutations before and during ADV treatment with a specificity of 100% and a sensitivity of 100%.展开更多
基金this project was provided by the Natural Science Foundation of China (Grant No.41271321)the National Key Basic Research Program of China (Grant no. 2012CB417101)
文摘Quantification of soil spatial and temporal variability at watershed scale is important in ecological modeling, precision agriculture, and natural resources management. The spatio-temporal variations of soil nitrogen under different land uses in a small watershed （12.10 km^2） in the hilly area of purple soil at the upper reaches of the Yangtze River in southwestern China were investigated by using conventional statistics, geostatistics, and a geographical information system in order to provide information for land management and control of environmental issues. A total of 552 soil samples （o to 15 cm） from 276 sites within the watershed were collected in April and August of 2o11, and analyzed for soil total nitrogen （STN） and nitrate nitrogen （NO3-N）. We compared spatial variations of STN and NO3-N under different land uses as well as the temporal variations in April （dry season） and August （rainy season）. Results showed that STN contents were deeply affected by land-use types; median STN values ranged from 0.94to 1.27g.kg-I, and STN contents decreased in the following order： paddy field 〉 foresfland 〉 sloping cropland. No significant difference was found for STN contents between April and August under the same land use. However, NO3- N contents were 23.26, 10.58, and 26.19 mg·kg^-1 in April, and 1.34, 8.51, and 3.00 mg·kg^-1 in August for the paddy field, sloping cropland and forestland, respectively. Nugget ratios for STN indicatedmoderate spatial dependence in the paddy field and sloping cropland, and a strong spatial dependence in forestland. The processes of nitrogen movement, transformation, absorption of plant were deeply influenced by land use types; as a result, great changes of soil nitrogen levels at spatial and temporal scales were demonstrated in the studied watershed.
基金Supported by The fund from Health Project of Jiangsu Province,No.H200711the AIDS,Hepatitis B and Other Infectious Diseases Prevention Program,No.2009ZX10004-712
文摘AIM:To establish the more feasible and sensitive assessment approach to the detection of adefovir (ADV) resistance-associated hepatitis B virus (HBV) quasispecies.METHODS: Based on the characteristics of rtA181V/T and rtN236T mutations, a new approach based on real-time fluorescent quantitative polymerase chain reaction (RT-PCR) was established for the detection of ADV-resistant HBV quasispecies, total HBV DNA, rtA181 and rtN236 mutations in blood samples from 32 chronic hepatitis B (CHB) patients with unsatisfactory curative effect on ADV and compared with routine HBV DNA sequencing.RESULTS: Both the sensitivity and specificity of this new detection approach to ADV-resistant HBV quasispecies were 100%, which were much higher than those of direct HBV DNA sequencing. The approach was able to detect 0.1% of mutated strains in a total plasmid population. Among the 32 clinical patients, single rtA181 and rtN236T mutation and double rtA181T and rtN236T mutations were detected in 20 and 8, respectively, while ADV-resistant mutations in 6 (including, rtA181V/T mutation alone in 5 patients) and no associated mutations in 26.CONCLUSION: This new approach is more feasible and efficient to detect ADV-resistant mutants of HBV and ADV-resistant mutations before and during ADV treatment with a specificity of 100% and a sensitivity of 100%.
