Pulsed plasma arc deposition （PPAD）, which combines pulsed plasma cladding with rapid prototyping, is a promising technology for manufacturing near net shape components due to its superiority in cost and convenience...Pulsed plasma arc deposition （PPAD）, which combines pulsed plasma cladding with rapid prototyping, is a promising technology for manufacturing near net shape components due to its superiority in cost and convenience of processing. In the present research, PPAD was successfully used to fabricate the Ni-based superalloy Inconel 625 components. The microstructures and mechanical properties of deposits were investigated by scanning electron microscopy （SEM）, optical microscopy （OM）, transmission electron microscopy （TEM） with energy dispersive spectrometer （EDS）, microhardness and tensile testers. It was found that the as-deposited structure exhibited homogenous columnar dendrite structure, which grew epitaxially along the deposition direction. Moreover, some intermetallic phases such as Laves phase, minor MC （NbC, TiC） carbides and needle-like δ-Ni3Nb were observed in y-Ni matrix. Precipitation mechanism and distribution characteristics of these intermetallic phases in the as-deposited 625 alloy sample were analyzed. In order to evaluate the mechanical properties of the deposits, microhardness was measured at various location （including transverse plane and longitudinal plane）. The results revealed hardness was in the range of 260- 285 HVo.2. In particular, microhardness at the interface region between two adjacent deposited layers was slightly higher than that at other regions due to highly refined structure and the disperse distribution of Laves particles. Finally, the influence of precipitation phases and fabrication strategies on the tensile properties of the as-deposited samples was investigated. The failure modes of the tensile specimens were analyzed with fractography.展开更多
Due to influence of compressibility,shock wave,instabilities,and turbulence on supersonic flows, current flow visualization and imaging techniques encounter some problems in high spatiotemporal resolution and high sig...Due to influence of compressibility,shock wave,instabilities,and turbulence on supersonic flows, current flow visualization and imaging techniques encounter some problems in high spatiotemporal resolution and high signal-to-noise ratio(SNR)measurements.Therefore,nanoparticle based planar laser scattering method(NPLS)is developed here.The nanoparticles are used as tracer,and pulse planar laser is used as light source in NPLS;by recording images of particles in flow field with CCD, high spatiotemporal resolution supersonic flow imaging is realized.The flow-following ability of nanoparticles in supersonic flows is studied according to multiphase flow theory and calibrating experiment of oblique shock wave.The laser scattering characteristics of nanoparticles are analyzed with light scattering theory.The results of theoretical and experimental studies show that the dynamic behavior and light scattering characteristics of nanoparticles highly enhance the spatiotemporal resolution and SNR of NPLS,with which the flow field involving shock wave,expansion,Mach disk,boundary layer,sliding-line,and mixing layer can be imaged clearly at high spatiotemporal resolution.展开更多
Magnesium alloys,the advanced lightweight structural materials,have been successfully applied in the manufacturing field.Unfortunately,their poor corrosion resistance restrains the potential wide applications.In this ...Magnesium alloys,the advanced lightweight structural materials,have been successfully applied in the manufacturing field.Unfortunately,their poor corrosion resistance restrains the potential wide applications.In this work,anti-corrosion coatings were fabricated via the insitu growth of the corrosion inhibitors intercalated magnesium-aluminum layered double hydroxide(Mg-Al LDH)on AZ31 magnesium alloy and then post-sealing it by a super-hydrophobic coating.SEM,XRD,EDS,FTIR,XPS and contact angle test were conducted to analyze physical/chemical features of these coatings.Potentiodynamic polarization curves and electrochemical impedance spectroscopy were recorded to assess the anti-corrosion performance of prepared coatings.Surprisingly,Mg-Al LDH with molybdate intercalation and lauric acid modification achieves the excellent corrosion inhibition performance(99.99%)due to the multicomponent synergistic effect such as the physical protection of Mg-Al LDH,the corrosion inhibition of molybdate and super-hydrophobic properties of lauric acid.This work presents a scientific perspective and novel design philosophy to fabricate the efficient anti-corrosion coating to protect magnesium alloys and then expand their potential applications in other field.展开更多
A one-step electrodeposition method was used to construct a superhydrophobic coating on the surface of magnesium alloy.Using magne-sium nitrate and ethanol solution of stearic acid as electrolyte,four groups of electr...A one-step electrodeposition method was used to construct a superhydrophobic coating on the surface of magnesium alloy.Using magne-sium nitrate and ethanol solution of stearic acid as electrolyte,four groups of electrolytes with different ratios of stearic acid and magnesium nitrate were designed to explore the influence of electrolyte to the process.The contact angles of the four coating samples were 136.4±5.8°,152.7±2.8°,156.2±4.9°and 155.1±4.4°The addition of magnesium nitrate in the solution increased the hydrogen evolution reaction in the electrodeposition process,which was not conducive to electrodeposition process.During the deposition process,the sample prepared in a molar ratio of stearic acid to magnesium nitrate of 10:1 had the best corrosion resistance,and its corrosion circuit density was 3.74×10^(−8)A/cm^(2),far lower than the corrosion current density of the magnesium alloy substrate.展开更多
Flow visualization of supersonic mixing layer has been studied based on the high spatiotemporal resolution Nano-based Planar Laser Scattering(NPLS) method in SML-1 wind tunnel. The corresponding images distinctly repr...Flow visualization of supersonic mixing layer has been studied based on the high spatiotemporal resolution Nano-based Planar Laser Scattering(NPLS) method in SML-1 wind tunnel. The corresponding images distinctly reproduced the flow structure of laminar,transitional and turbulent region,with which the fractal measurement can be implemented. Two methods of measuring fractal dimension were introduced and compared. The fractal dimension of the transitional region and the fully developing turbulence region of supersonic mixing layer were measured based on the box-counting method. In the transitional region,the fractal dimension will increase with turbulent intensity. In the fully developing turbulent region,the fractal dimension will not vary apparently for different flow structures,which em-bodies the self-similarity of supersonic turbulence.展开更多
The inflammatory response is induced by the overexpression of inflammatory cytokines, mainly interleukin(IL)-1β, and is one of the main causes of intervertebral disc degeneration(IVDD). NLR pyrin domain containing 3(...The inflammatory response is induced by the overexpression of inflammatory cytokines, mainly interleukin(IL)-1β, and is one of the main causes of intervertebral disc degeneration(IVDD). NLR pyrin domain containing 3(NLRP3) inflammasome activation is an important source of IL-1β. As an anti-inflammatory neuroendocrine hormone, melatonin plays various roles in different pathophysiological conditions. However, its roles in IVDD are still not well understood and require more examination. First, we demonstrated that melatonin delayed the progression of IVDD and relieved IVDD-related low back pain in a rat needle puncture IVDD model;moreover, NLRP3 inflammasome activation(NLRP3, p20, and IL-1β levels) was significantly upregulated in severely degenerated human discs and a rat IVDD model. Subsequently, an IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop was found in nucleus pulposus(NP) cells that were treated with IL-1β. In these cells, expression of NLRP3 and p20 was significantly increased, NF-κB signaling was involved in this regulation, and mitochondrial reactive oxygen species(mt ROS)production increased. Furthermore, we found that melatonin disrupted the IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop in vitro and in vivo. Melatonin treatment decreased NLRP3, p20, and IL-1β levels by inhibiting NF-κB signaling and downregulating mt ROS production. Finally, we showed that melatonin mediated the disruption of the positive feedback loop of IL-1β in vivo. In this study, we showed for the first time that IL-1β promotes its own expression by upregulating NLRP3 inflammasome activation. Furthermore, melatonin disrupts the IL-1β positive feedback loop and may be a potential therapeutic agent for IVDD.展开更多
氮素营养诊断关键在于氮营养指数(nitrogen nutrient index,NNI)预测。对于冬小麦氮营养指数预测模型而言,如何选取预处理方法和建模方法不一而足,不同预处理和模型选取对预测结果精度的影响程度目前还不清楚。该研究以ASD Field Spec3...氮素营养诊断关键在于氮营养指数(nitrogen nutrient index,NNI)预测。对于冬小麦氮营养指数预测模型而言,如何选取预处理方法和建模方法不一而足,不同预处理和模型选取对预测结果精度的影响程度目前还不清楚。该研究以ASD Field Spec3野外便携式高光谱仪采集乐陵市冬小麦冠层高光谱数据,采用10种光谱预处理方法并结合3种模型(偏最小二乘回归、BP神经网络和随机森林算法)建立多种冬小麦氮营养指数高光谱预测模型。对比模型预测精度表明最佳的高光谱建模方法为随机森林算法结合SG卷积平滑预处理所建模型(预测集R2=0.795,RMSE=0.125,RE=11.7%)精度高、可靠性强,是筛选出最佳的冬小麦氮营养指数高光谱预测模型。该研究结果对冬小麦氮营养指数高光谱预测建模具有科学价值,为筛选最优高光谱预处理方法和预测模型提供技术参考。展开更多
Antimicrobial peptides(AMPs),with their extraordinary properties,such as broad-spectrum activity,rapid action and difficult development of resistance,have become promising molecules as new antibiotics.Despite their va...Antimicrobial peptides(AMPs),with their extraordinary properties,such as broad-spectrum activity,rapid action and difficult development of resistance,have become promising molecules as new antibiotics.Despite their various mechanisms of action,the interaction of AMPs with the bacterial cell membrane is the key step for their mode of action.Moreover,it is generally accepted that the membrane is the primary target of most AMPs,and the interaction between AMPs and eukaryotic cell membranes(causing toxicity to host cells)limits their clinical application.Therefore,researchers are engaged in reforming or de novo designing AMPs as a‘singleedged sword’that contains high antimicrobial activity yet low cytotoxicity against eukaryotic cells.To improve the antimicrobial activity of AMPs,the relationship between the structure and function of AMPs has been rigorously pursued.In this review,we focus on the current knowledge of α-helical cationic antimicrobial peptides,one of the most common types of AMPs in nature.展开更多
Growth inhibition and cold-acclimation strategies help plants withstand cold stress,which adversely affects growth and survival.PHYTOCHROME B(phyB)regulates plant growth through perceiving both light and ambient tempe...Growth inhibition and cold-acclimation strategies help plants withstand cold stress,which adversely affects growth and survival.PHYTOCHROME B(phyB)regulates plant growth through perceiving both light and ambient temperature signals.However,the mechanism by which phyB mediates the plant response to cold stress remains elusive.Here,we show that the key transcription factors mediating cold acclimation,C-REPEAT BINDING FACTORs(CBFs),interact with PHYTOCHROME-INTERACTING FACTOR 3(PIF3)under cold stress,thus attenuating the mutually assured destruction of PIF3–phyB.Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related genes.Consistent with this,phyB mutants exhibited a freezing-sensitive phenotype,whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance.Further analysis showed that the PIF1,PIF4,and PIF5 proteins,all of which negatively regulate plant freezing tolerance,were destabilized by cold stress in a phytochrome-dependent manner.Collectively,our study reveals that CBFs–PIF3–phyB serves as an important regulatory module for modulating plant response to cold stress.展开更多
The purpose of this study was to determine perfluoroalkyl substances（PFASs） in human serum, urine, hair and nail from general populations, and to investigate the possibility for human urine, hair and nail used as th...The purpose of this study was to determine perfluoroalkyl substances（PFASs） in human serum, urine, hair and nail from general populations, and to investigate the possibility for human urine, hair and nail used as the biomonitoring sample for PFASs exposure. We detected the concentrations of PFHx A, PFOA, PFNA, PFDA, PFUn DA, PFDo A, PFHx S and PFOS in 39 matched human serum, urine, hair and nail samples from Shenzhen in China. The detection frequency and the median level of PFOS were all higher than that of the other PFASs in four matrices. The median concentration of PFOS in serum, urine, hair, and nail were 9.24 ng/mL, 13.96 ng/L, 0.58 ng/g and 0.63 ng/g, respectively. The results of spearman correlation test indicated that nail was an ideal matrix for biomonitoring PFOS rather than human urine and hair in general populations for the non-invasive sampling.展开更多
The existing methods for measuring aero-optical aberration suffer from several problems,such as low spatiotemporal resolution,sensitivity to environment,and integral effects.A new method for measuring aero-optical abe...The existing methods for measuring aero-optical aberration suffer from several problems,such as low spatiotemporal resolution,sensitivity to environment,and integral effects.A new method for measuring aero-optical aberration of supersonic flow is proposed.Based on the self-developed measuring method of supersonic density field,the wavefront aberration induced by a cross-section of supersonic flow field could be measured by ray-tracing.Compared with other methods,the present one has three significant innovations:(1) high spatiotemporal resolution.Its time resolution is 6 ns,and the spatial resolution can reach up to micrometers;(2) it can avoid the integral effects and study the wavefront aberration induced by the flow field of interest locally;(3) it can also avoid the influence from the test section wall boundary layers and environmental disturbances.The present method was applied to supersonic flow around an optical bow cap.The results of high spatiotemporal resolution reveal fine wavefront structures,and show that shock waves,expansion waves and turbulent boundary layers have different impacts on the wavefront aberration.展开更多
Flow in a flat-plate zero-pressure-gradient boundary layer at Mach 3 was visualized via nanoparticle-based planar laser scattering (NPLS). Coherent structures such as an individual hairpin vortex and hairpin packet we...Flow in a flat-plate zero-pressure-gradient boundary layer at Mach 3 was visualized via nanoparticle-based planar laser scattering (NPLS). Coherent structures such as an individual hairpin vortex and hairpin packet were identified in the streamwise-wall-normal plane on the basis of the now accepted hairpin model. Λ-shaped vortices were found in a staggered pattern in the stream-wise-spanwise plane, which indicated H-type transition in the present experiments. This is the direct evidence (in the form of flow visualization) of such coherent structures in a supersonic boundary layer. A series of NPLS images taken in streamwise-spanwise planes at different heights is presented, and the three-dimensional structures of the supersonic boundary layer agree well with the hairpin model.展开更多
Aqueous zinc-ion batteries(AZIBs)are one of the promising energy storage systems,which consist of electrode materials,electrolyte,and separator.The first two have been significantly received ample development,while th...Aqueous zinc-ion batteries(AZIBs)are one of the promising energy storage systems,which consist of electrode materials,electrolyte,and separator.The first two have been significantly received ample development,while the prominent role of the separators in manipulating the stability of the electrode has not attracted sufficient attention.In this work,a separator(UiO-66-GF)modified by Zr-based metal organic framework for robust AZIBs is proposed.UiO-66-GF effectively enhances the transport ability of charge carriers and demonstrates preferential orientation of(002)crystal plane,which is favorable for corrosion resistance and dendrite-free zinc deposition.Consequently,Zn|UiO-66-GF-2.2|Zn cells exhibit highly reversible plating/stripping behavior with long cycle life over 1650 h at 2.0 mA cm^(−2),and Zn|UiO-66-GF-2.2|MnO_(2) cells show excellent long-term stability with capacity retention of 85%after 1000 cycles.The reasonable design and application of multifunctional metal organic frameworks modified separators provide useful guidance for constructing durable AZIBs.展开更多
The interaction between shock wave and turbulence has been studied in supersonic turbulent mix layer wind tunnel. The interaction between oblique shock wave and turbulent boundary layer and the influence of large vort...The interaction between shock wave and turbulence has been studied in supersonic turbulent mix layer wind tunnel. The interaction between oblique shock wave and turbulent boundary layer and the influence of large vortex in mix layer on oblique shock wave have been observed by NPLS technique. From NPLS image, not only complex flow structure is observed but also time-dependent supersonic flow visualization is realized. The mechanism of interaction between shock wave and turbulence is discussed based on high quality NPLS image.展开更多
Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, ...Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-adenosylmethionine decarboxylases （SAMDCs） necessary for the formation of the indispensable intermediate in the polyamine biosynthetic pathway. The bud2 plant has enlarged vascular systems in inflorescences, roots, and petioles, and an altered homeostasis ofpolyamines. The double mutant of bud2 and samdcl, a knockdown mutant of another SAMDC member, is embryo lethal, demonstrating that SAMDCs are essential for plant embryogenesis. Our results suggest that polyamines are required for the normal growth and development of higher plants.展开更多
A type of specific fractured hydrocarbon reservoir, a shale fractured reservoir, exists in the Shengli oilfield. Due to very small porosity of this type, low sensitivity to the variation of petrochemical property para...A type of specific fractured hydrocarbon reservoir, a shale fractured reservoir, exists in the Shengli oilfield. Due to very small porosity of this type, low sensitivity to the variation of petrochemical property parameters, and strong anisotropy, it is very difficult to explore for them. So far, there is no set of mature methods for recognition of direction, distribution, and density of the fractures by an integrated analysis of geologic, geophysical, well log, drilling data, and etc. This paper presents a new method for acoustic impedance variation with azimuth （IPVA）, based on existing fracture detection methods. Seismic acquisition, processing, and recognition techniques were developed for detecting directional vertical fractures using multi-azimuth P wave data in combination with the seismic and geological features of shale fractures in the Luojia area. The IPVA research is carried out for recognizing the distribution, strike, and density of fractures based on the study of velocity variation with azimuth （VVA） and amplitude variation with azimuth （AVA） for full azimuth P wave data at different CMP positions. Through practical application in the Luojia area, primary results have been obtained which verifies that the IPVA method provides good potential for quantitative detection of parallel, high angle, shale fractures.展开更多
Lithium-ion batteries(LIBs)have been widely applied in portable electronic devices and electric vehicles.With the booming of the respective markets,a huge quantity of spent LIBs that typically use either LiFePO_(4) or...Lithium-ion batteries(LIBs)have been widely applied in portable electronic devices and electric vehicles.With the booming of the respective markets,a huge quantity of spent LIBs that typically use either LiFePO_(4) or Li N_(x)Co_(y)Mn_(z)O_(2) cathode materials will be produced in the very near future,imposing significant pressure for the development of suitable disposal/recycling technologies,in terms of both environmental protection and resource reclaiming.In this review,we firstly do a comprehensive summary of the-state-of-art technologies to recycle Li N_(x)Co_(y)Mn_(z)O_(2) and LiFePO_(4)-based LIBs,in the aspects of pretreatment,hydrometallurgical recycling,and direct regeneration of the cathode materials.This closed-loop strategy for cycling cathode materials has been regarded as an ideal approach considering its economic benefit and environmental friendliness.Afterward,as for the exhausted anode materials,we focus on the utilization of exhausted anode materials to obtain other functional materials,such as graphene.Finally,the existing challenges in recycling the LiFePO_(4) and Li N_(x)Co_(y)Mn_(z)O_(2) cathodes and graphite anodes for industrial-scale application are discussed in detail;and the possible strategies for these issues are proposed.We expect this review can provide a roadmap towards better technologies for recycling LIBs,shed light on the future development of novel battery recycling technologies to promote the environmental benignity and economic viability of the battery industry and pave way for the large-scale application of LIBs in industrial fields in the near future.展开更多
基金supported by the National Basic Research Program of China("973 Project",No.2011CB013403)the National Science and Technology Supporting Project (Nos.2011BAF11B07 and 2011BAC10B05)
文摘Pulsed plasma arc deposition （PPAD）, which combines pulsed plasma cladding with rapid prototyping, is a promising technology for manufacturing near net shape components due to its superiority in cost and convenience of processing. In the present research, PPAD was successfully used to fabricate the Ni-based superalloy Inconel 625 components. The microstructures and mechanical properties of deposits were investigated by scanning electron microscopy （SEM）, optical microscopy （OM）, transmission electron microscopy （TEM） with energy dispersive spectrometer （EDS）, microhardness and tensile testers. It was found that the as-deposited structure exhibited homogenous columnar dendrite structure, which grew epitaxially along the deposition direction. Moreover, some intermetallic phases such as Laves phase, minor MC （NbC, TiC） carbides and needle-like δ-Ni3Nb were observed in y-Ni matrix. Precipitation mechanism and distribution characteristics of these intermetallic phases in the as-deposited 625 alloy sample were analyzed. In order to evaluate the mechanical properties of the deposits, microhardness was measured at various location （including transverse plane and longitudinal plane）. The results revealed hardness was in the range of 260- 285 HVo.2. In particular, microhardness at the interface region between two adjacent deposited layers was slightly higher than that at other regions due to highly refined structure and the disperse distribution of Laves particles. Finally, the influence of precipitation phases and fabrication strategies on the tensile properties of the as-deposited samples was investigated. The failure modes of the tensile specimens were analyzed with fractography.
基金Supported by the National Natural Science Foundation of China(Grant No.10672178)
文摘Due to influence of compressibility,shock wave,instabilities,and turbulence on supersonic flows, current flow visualization and imaging techniques encounter some problems in high spatiotemporal resolution and high signal-to-noise ratio(SNR)measurements.Therefore,nanoparticle based planar laser scattering method(NPLS)is developed here.The nanoparticles are used as tracer,and pulse planar laser is used as light source in NPLS;by recording images of particles in flow field with CCD, high spatiotemporal resolution supersonic flow imaging is realized.The flow-following ability of nanoparticles in supersonic flows is studied according to multiphase flow theory and calibrating experiment of oblique shock wave.The laser scattering characteristics of nanoparticles are analyzed with light scattering theory.The results of theoretical and experimental studies show that the dynamic behavior and light scattering characteristics of nanoparticles highly enhance the spatiotemporal resolution and SNR of NPLS,with which the flow field involving shock wave,expansion,Mach disk,boundary layer,sliding-line,and mixing layer can be imaged clearly at high spatiotemporal resolution.
基金This work is financially supported by the Graduate Research and Innovation of Chongqing,China(Grant No.CYB18002)the National Natural Science Foundation of China(Grant No.21576034)+2 种基金the State Education Ministry and Fundamental Research Funds for the Central Universities(2019CDQYCL042,106112017CDJXSYY0001,2018CDYJSY0055,106112017CDJQJ138802,106112017CDJSK04XK11,2018CDQYCL0027)the Joint Funds of the National Natural Science Foundation of China-Guangdong(Grant No.U1801254)Fundamental Re-search Funds for the Central Universities(NO.2018CDJDCD0001).
文摘Magnesium alloys,the advanced lightweight structural materials,have been successfully applied in the manufacturing field.Unfortunately,their poor corrosion resistance restrains the potential wide applications.In this work,anti-corrosion coatings were fabricated via the insitu growth of the corrosion inhibitors intercalated magnesium-aluminum layered double hydroxide(Mg-Al LDH)on AZ31 magnesium alloy and then post-sealing it by a super-hydrophobic coating.SEM,XRD,EDS,FTIR,XPS and contact angle test were conducted to analyze physical/chemical features of these coatings.Potentiodynamic polarization curves and electrochemical impedance spectroscopy were recorded to assess the anti-corrosion performance of prepared coatings.Surprisingly,Mg-Al LDH with molybdate intercalation and lauric acid modification achieves the excellent corrosion inhibition performance(99.99%)due to the multicomponent synergistic effect such as the physical protection of Mg-Al LDH,the corrosion inhibition of molybdate and super-hydrophobic properties of lauric acid.This work presents a scientific perspective and novel design philosophy to fabricate the efficient anti-corrosion coating to protect magnesium alloys and then expand their potential applications in other field.
基金The authors are very grateful for the support from the National Key Research and Development Program of China(No.2016YFB0301102)the Fundamental Research Funds for the Central Universities(No.2018CDGFCL0005)the Postgraduate Education Fund of Chongqing University(No.201704020).
文摘A one-step electrodeposition method was used to construct a superhydrophobic coating on the surface of magnesium alloy.Using magne-sium nitrate and ethanol solution of stearic acid as electrolyte,four groups of electrolytes with different ratios of stearic acid and magnesium nitrate were designed to explore the influence of electrolyte to the process.The contact angles of the four coating samples were 136.4±5.8°,152.7±2.8°,156.2±4.9°and 155.1±4.4°The addition of magnesium nitrate in the solution increased the hydrogen evolution reaction in the electrodeposition process,which was not conducive to electrodeposition process.During the deposition process,the sample prepared in a molar ratio of stearic acid to magnesium nitrate of 10:1 had the best corrosion resistance,and its corrosion circuit density was 3.74×10^(−8)A/cm^(2),far lower than the corrosion current density of the magnesium alloy substrate.
基金the National Natural Science Foundation of China (Grant No. 10672178)
文摘Flow visualization of supersonic mixing layer has been studied based on the high spatiotemporal resolution Nano-based Planar Laser Scattering(NPLS) method in SML-1 wind tunnel. The corresponding images distinctly reproduced the flow structure of laminar,transitional and turbulent region,with which the fractal measurement can be implemented. Two methods of measuring fractal dimension were introduced and compared. The fractal dimension of the transitional region and the fully developing turbulence region of supersonic mixing layer were measured based on the box-counting method. In the transitional region,the fractal dimension will increase with turbulent intensity. In the fully developing turbulent region,the fractal dimension will not vary apparently for different flow structures,which em-bodies the self-similarity of supersonic turbulence.
基金supported by grants from the Natural Science Foundation of Guangdong Province (Grant no: 2017A030313670)the National Natural Science Foundation of China (81572175 and 81772386)。
文摘The inflammatory response is induced by the overexpression of inflammatory cytokines, mainly interleukin(IL)-1β, and is one of the main causes of intervertebral disc degeneration(IVDD). NLR pyrin domain containing 3(NLRP3) inflammasome activation is an important source of IL-1β. As an anti-inflammatory neuroendocrine hormone, melatonin plays various roles in different pathophysiological conditions. However, its roles in IVDD are still not well understood and require more examination. First, we demonstrated that melatonin delayed the progression of IVDD and relieved IVDD-related low back pain in a rat needle puncture IVDD model;moreover, NLRP3 inflammasome activation(NLRP3, p20, and IL-1β levels) was significantly upregulated in severely degenerated human discs and a rat IVDD model. Subsequently, an IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop was found in nucleus pulposus(NP) cells that were treated with IL-1β. In these cells, expression of NLRP3 and p20 was significantly increased, NF-κB signaling was involved in this regulation, and mitochondrial reactive oxygen species(mt ROS)production increased. Furthermore, we found that melatonin disrupted the IL-1β/NF-κB-NLRP3 inflammasome activation positive feedback loop in vitro and in vivo. Melatonin treatment decreased NLRP3, p20, and IL-1β levels by inhibiting NF-κB signaling and downregulating mt ROS production. Finally, we showed that melatonin mediated the disruption of the positive feedback loop of IL-1β in vivo. In this study, we showed for the first time that IL-1β promotes its own expression by upregulating NLRP3 inflammasome activation. Furthermore, melatonin disrupts the IL-1β positive feedback loop and may be a potential therapeutic agent for IVDD.
文摘氮素营养诊断关键在于氮营养指数(nitrogen nutrient index,NNI)预测。对于冬小麦氮营养指数预测模型而言,如何选取预处理方法和建模方法不一而足,不同预处理和模型选取对预测结果精度的影响程度目前还不清楚。该研究以ASD Field Spec3野外便携式高光谱仪采集乐陵市冬小麦冠层高光谱数据,采用10种光谱预处理方法并结合3种模型(偏最小二乘回归、BP神经网络和随机森林算法)建立多种冬小麦氮营养指数高光谱预测模型。对比模型预测精度表明最佳的高光谱建模方法为随机森林算法结合SG卷积平滑预处理所建模型(预测集R2=0.795,RMSE=0.125,RE=11.7%)精度高、可靠性强,是筛选出最佳的冬小麦氮营养指数高光谱预测模型。该研究结果对冬小麦氮营养指数高光谱预测建模具有科学价值,为筛选最优高光谱预处理方法和预测模型提供技术参考。
基金This work was supported by Natural Science Foundation of China Grant No.30840029Grants for Doctorate Program of New Teacher,Ministry of Education of China,No.20070183062,No.200801831064the Excellent Youth Program of the Jilin Provincial Science&Technology Department,China,Grant No.20070111.
文摘Antimicrobial peptides(AMPs),with their extraordinary properties,such as broad-spectrum activity,rapid action and difficult development of resistance,have become promising molecules as new antibiotics.Despite their various mechanisms of action,the interaction of AMPs with the bacterial cell membrane is the key step for their mode of action.Moreover,it is generally accepted that the membrane is the primary target of most AMPs,and the interaction between AMPs and eukaryotic cell membranes(causing toxicity to host cells)limits their clinical application.Therefore,researchers are engaged in reforming or de novo designing AMPs as a‘singleedged sword’that contains high antimicrobial activity yet low cytotoxicity against eukaryotic cells.To improve the antimicrobial activity of AMPs,the relationship between the structure and function of AMPs has been rigorously pursued.In this review,we focus on the current knowledge of α-helical cationic antimicrobial peptides,one of the most common types of AMPs in nature.
基金This work was supported by grants from the Ministry of Agriculture of China,China(2016ZX08009003-002)the National Natural Science Foundation of China,China(31872658,31921001)Discipline Program of Beijing Outstanding University,China.
文摘Growth inhibition and cold-acclimation strategies help plants withstand cold stress,which adversely affects growth and survival.PHYTOCHROME B(phyB)regulates plant growth through perceiving both light and ambient temperature signals.However,the mechanism by which phyB mediates the plant response to cold stress remains elusive.Here,we show that the key transcription factors mediating cold acclimation,C-REPEAT BINDING FACTORs(CBFs),interact with PHYTOCHROME-INTERACTING FACTOR 3(PIF3)under cold stress,thus attenuating the mutually assured destruction of PIF3–phyB.Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related genes.Consistent with this,phyB mutants exhibited a freezing-sensitive phenotype,whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance.Further analysis showed that the PIF1,PIF4,and PIF5 proteins,all of which negatively regulate plant freezing tolerance,were destabilized by cold stress in a phytochrome-dependent manner.Collectively,our study reveals that CBFs–PIF3–phyB serves as an important regulatory module for modulating plant response to cold stress.
基金funded by the National Natural Science of Foundation of China(Nos.21537001 and 21407033)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB14010100)
文摘The purpose of this study was to determine perfluoroalkyl substances（PFASs） in human serum, urine, hair and nail from general populations, and to investigate the possibility for human urine, hair and nail used as the biomonitoring sample for PFASs exposure. We detected the concentrations of PFHx A, PFOA, PFNA, PFDA, PFUn DA, PFDo A, PFHx S and PFOS in 39 matched human serum, urine, hair and nail samples from Shenzhen in China. The detection frequency and the median level of PFOS were all higher than that of the other PFASs in four matrices. The median concentration of PFOS in serum, urine, hair, and nail were 9.24 ng/mL, 13.96 ng/L, 0.58 ng/g and 0.63 ng/g, respectively. The results of spearman correlation test indicated that nail was an ideal matrix for biomonitoring PFOS rather than human urine and hair in general populations for the non-invasive sampling.
基金supported by the National Natural Science Foundation of China (10672178, 10772168)National Basic Research Program of China (2009CB724100)
文摘The existing methods for measuring aero-optical aberration suffer from several problems,such as low spatiotemporal resolution,sensitivity to environment,and integral effects.A new method for measuring aero-optical aberration of supersonic flow is proposed.Based on the self-developed measuring method of supersonic density field,the wavefront aberration induced by a cross-section of supersonic flow field could be measured by ray-tracing.Compared with other methods,the present one has three significant innovations:(1) high spatiotemporal resolution.Its time resolution is 6 ns,and the spatial resolution can reach up to micrometers;(2) it can avoid the integral effects and study the wavefront aberration induced by the flow field of interest locally;(3) it can also avoid the influence from the test section wall boundary layers and environmental disturbances.The present method was applied to supersonic flow around an optical bow cap.The results of high spatiotemporal resolution reveal fine wavefront structures,and show that shock waves,expansion waves and turbulent boundary layers have different impacts on the wavefront aberration.
基金supported by the National Basic Research Program of China (2009CB724100)
文摘Flow in a flat-plate zero-pressure-gradient boundary layer at Mach 3 was visualized via nanoparticle-based planar laser scattering (NPLS). Coherent structures such as an individual hairpin vortex and hairpin packet were identified in the streamwise-wall-normal plane on the basis of the now accepted hairpin model. Λ-shaped vortices were found in a staggered pattern in the stream-wise-spanwise plane, which indicated H-type transition in the present experiments. This is the direct evidence (in the form of flow visualization) of such coherent structures in a supersonic boundary layer. A series of NPLS images taken in streamwise-spanwise planes at different heights is presented, and the three-dimensional structures of the supersonic boundary layer agree well with the hairpin model.
基金This work was supported by the National Natural Science Foundation of China(Nos.51872090,51972346)the Hebei Natural Science Fund for Distinguished Young Scholar(No.E2019209433)+2 种基金the Natural Science Foundation of Hebei Province(No.E2020209151)the Hunan Natural Science Fund for Distinguished Young Scholar(2021JJ10064)the Program of Youth Talent Support for Hunan Province(2020RC3011).
文摘Aqueous zinc-ion batteries(AZIBs)are one of the promising energy storage systems,which consist of electrode materials,electrolyte,and separator.The first two have been significantly received ample development,while the prominent role of the separators in manipulating the stability of the electrode has not attracted sufficient attention.In this work,a separator(UiO-66-GF)modified by Zr-based metal organic framework for robust AZIBs is proposed.UiO-66-GF effectively enhances the transport ability of charge carriers and demonstrates preferential orientation of(002)crystal plane,which is favorable for corrosion resistance and dendrite-free zinc deposition.Consequently,Zn|UiO-66-GF-2.2|Zn cells exhibit highly reversible plating/stripping behavior with long cycle life over 1650 h at 2.0 mA cm^(−2),and Zn|UiO-66-GF-2.2|MnO_(2) cells show excellent long-term stability with capacity retention of 85%after 1000 cycles.The reasonable design and application of multifunctional metal organic frameworks modified separators provide useful guidance for constructing durable AZIBs.
文摘The interaction between shock wave and turbulence has been studied in supersonic turbulent mix layer wind tunnel. The interaction between oblique shock wave and turbulent boundary layer and the influence of large vortex in mix layer on oblique shock wave have been observed by NPLS technique. From NPLS image, not only complex flow structure is observed but also time-dependent supersonic flow visualization is realized. The mechanism of interaction between shock wave and turbulence is discussed based on high quality NPLS image.
基金We thank Dr Senya Matsufuji(Jikei University,Japan)for providing the yeast spe2 strain,Dr Guixian Xia(Institute of Microbiology,Chinese Academy of Sciences,China)for yeast expression vector pREP5N and ABRC stock center for BAC clones(F17K4 and T16G12)and T-DNA insertion lines(Salk_007279 and Salk_020185).This work was supported by grants from National Natural Science Foundation of China(30330040)the Ministry of Sciences and Technology of China(J02-A-001).
文摘Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-adenosylmethionine decarboxylases （SAMDCs） necessary for the formation of the indispensable intermediate in the polyamine biosynthetic pathway. The bud2 plant has enlarged vascular systems in inflorescences, roots, and petioles, and an altered homeostasis ofpolyamines. The double mutant of bud2 and samdcl, a knockdown mutant of another SAMDC member, is embryo lethal, demonstrating that SAMDCs are essential for plant embryogenesis. Our results suggest that polyamines are required for the normal growth and development of higher plants.
文摘A type of specific fractured hydrocarbon reservoir, a shale fractured reservoir, exists in the Shengli oilfield. Due to very small porosity of this type, low sensitivity to the variation of petrochemical property parameters, and strong anisotropy, it is very difficult to explore for them. So far, there is no set of mature methods for recognition of direction, distribution, and density of the fractures by an integrated analysis of geologic, geophysical, well log, drilling data, and etc. This paper presents a new method for acoustic impedance variation with azimuth （IPVA）, based on existing fracture detection methods. Seismic acquisition, processing, and recognition techniques were developed for detecting directional vertical fractures using multi-azimuth P wave data in combination with the seismic and geological features of shale fractures in the Luojia area. The IPVA research is carried out for recognizing the distribution, strike, and density of fractures based on the study of velocity variation with azimuth （VVA） and amplitude variation with azimuth （AVA） for full azimuth P wave data at different CMP positions. Through practical application in the Luojia area, primary results have been obtained which verifies that the IPVA method provides good potential for quantitative detection of parallel, high angle, shale fractures.
基金supported by the National Natural Science Foundation of China(Nos.51072130,51502045 and 21905202)the Australian Research Council(ARC)through Discovery Early Career Researcher Award(DECRA,No.DE170100871)program。
文摘Lithium-ion batteries(LIBs)have been widely applied in portable electronic devices and electric vehicles.With the booming of the respective markets,a huge quantity of spent LIBs that typically use either LiFePO_(4) or Li N_(x)Co_(y)Mn_(z)O_(2) cathode materials will be produced in the very near future,imposing significant pressure for the development of suitable disposal/recycling technologies,in terms of both environmental protection and resource reclaiming.In this review,we firstly do a comprehensive summary of the-state-of-art technologies to recycle Li N_(x)Co_(y)Mn_(z)O_(2) and LiFePO_(4)-based LIBs,in the aspects of pretreatment,hydrometallurgical recycling,and direct regeneration of the cathode materials.This closed-loop strategy for cycling cathode materials has been regarded as an ideal approach considering its economic benefit and environmental friendliness.Afterward,as for the exhausted anode materials,we focus on the utilization of exhausted anode materials to obtain other functional materials,such as graphene.Finally,the existing challenges in recycling the LiFePO_(4) and Li N_(x)Co_(y)Mn_(z)O_(2) cathodes and graphite anodes for industrial-scale application are discussed in detail;and the possible strategies for these issues are proposed.We expect this review can provide a roadmap towards better technologies for recycling LIBs,shed light on the future development of novel battery recycling technologies to promote the environmental benignity and economic viability of the battery industry and pave way for the large-scale application of LIBs in industrial fields in the near future.
