Background:This study aimed to develop a comprehensive instrument for evaluating and ranking clinical practice guidelines,named Scientific,Transparent and Applicable Rankings tool(STAR),and test its reliability,validi...Background:This study aimed to develop a comprehensive instrument for evaluating and ranking clinical practice guidelines,named Scientific,Transparent and Applicable Rankings tool(STAR),and test its reliability,validity,and usability.Methods:This study set up a multidisciplinary working group including guideline methodologists,statisticians,journal editors,clinicians,and other experts.Scoping review,Delphi methods,and hierarchical analysis were used to develop the STAR tool.We evaluated the instrument’s intrinsic and interrater reliability,content and criterion validity,and usability.Results:STAR contained 39 items grouped into 11 domains.The mean intrinsic reliability of the domains,indicated by Cronbach’sαcoefficient,was 0.588(95%confidence interval[CI]:0.414,0.762).Interrater reliability as assessed with Cohen’s kappa coefficient was 0.774(95%CI:0.740,0.807)for methodological evaluators and 0.618(95%CI:0.587,0.648)for clinical evaluators.The overall content validity index was 0.905.Pearson’s r correlation for criterion validity was 0.885(95%CI:0.804,0.932).The mean usability score of the items was 4.6 and the median time spent to evaluate each guideline was 20 min.Conclusion:The instrument performed well in terms of reliability,validity,and efficiency,and can be used for comprehensively evaluating and ranking guidelines.展开更多
目的研发指南解读类文献的报告规范,以期更好地帮助指南解读者报告指南重要信息。方法RIGHT for INT课题组参考EQUATOR网站推荐的报告规范制订方法,确定了3个阶段的制订流程:第一阶段,通过邮件和微信的途径招募多学科的专家,根据职能划...目的研发指南解读类文献的报告规范,以期更好地帮助指南解读者报告指南重要信息。方法RIGHT for INT课题组参考EQUATOR网站推荐的报告规范制订方法,确定了3个阶段的制订流程:第一阶段,通过邮件和微信的途径招募多学科的专家,根据职能划分成核心制订小组、共识专家组及秘书组3个小组;第二阶段,采用文献调研和头脑风暴的方法收集初始条目;第三阶段,通过德尔菲调查和专家意见咨询,形成最终的RIGHT for INT清单。结果通过核心小组头脑风暴讨论和文献回顾,共收集40个初始条目。经过德尔菲调查和专家意见咨询过程(两轮均为17位专家,回复率均为100%),最终形成27个条目的RIGHT for INT清单。结论RIGHT for INT报告清单为指南解读者提供了撰写指南解读类文章的详细指导。展开更多
目的了解沙盘游戏疗法对少年肾病综合征患儿心理特征、社交焦虑及生活质量的影响。方法采用便利抽样方法抽取武汉市某三级甲等医院2018年5月-2021年5月收治的700例少年肾病综合征患儿为研究对象,将其随机分为观察组和对照组,每组350例,...目的了解沙盘游戏疗法对少年肾病综合征患儿心理特征、社交焦虑及生活质量的影响。方法采用便利抽样方法抽取武汉市某三级甲等医院2018年5月-2021年5月收治的700例少年肾病综合征患儿为研究对象,将其随机分为观察组和对照组,每组350例,其中对照组给予常规干预(即入院时介绍院内相关情况及疾病注意事项),观察组在对照组基础上实施沙盘游戏疗法。观察两组患儿干预前后应用Conners父母症状问卷(Parent Symptom Questionnaire,PSQ)、儿少心理健康量表(Mental Health Scale for Child and Adolescent,MHS-CA)、儿童社交焦虑量表(Social Anxiety Scale for Children,SASC)、儿少主观生活质量问卷(Inventory of Subjective Life Quality,ISLQ)评分。结果观察组干预后PSQ评分中各维度评分均明显低于对照组(P<0.05,Cohen's d>0.8),观察组干预后MHS-CA评分中各维度评分均明显高于对照组(P<0.05,Cohen's d>0.8),观察组干预后SASC评分中各维度评分均明显低于对照组(P<0.05,Cohen's d>0.8),观察组干预后ISLQ评分中各维度评分均明显高于对照组(P<0.05,Cohen's d>0.8)。结论沙盘游戏疗法能明显改善少年肾病综合征患儿的心理健康程度,缓解其社交焦虑,提升日常生活质量。展开更多
Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonst...Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis.展开更多
Summary What is already known about this topic?The quadrivalent influenza vaccine(QIV)provides protection against a broader range of influenza strains by including strains of influenza A/H1N1,A/H3N2,B/Yamagata,and B/V...Summary What is already known about this topic?The quadrivalent influenza vaccine(QIV)provides protection against a broader range of influenza strains by including strains of influenza A/H1N1,A/H3N2,B/Yamagata,and B/Victoria.What is added by this report?This study aimed to assess the immunogenicity and safety of administering a single dose compared to two doses of QIV in children,taking into consideration their previous influenza vaccination history.展开更多
高分辨质谱技术(High resolution mass spectrometer,HRMS)是具有食品安全风险识别能力的分析技术,理论上能够检测和鉴别食品中所有风险物质,适用于多种微量危害因子的鉴别和监测。基于HRMS的靶向高通量检测、非靶向筛查、代谢组学、分...高分辨质谱技术(High resolution mass spectrometer,HRMS)是具有食品安全风险识别能力的分析技术,理论上能够检测和鉴别食品中所有风险物质,适用于多种微量危害因子的鉴别和监测。基于HRMS的靶向高通量检测、非靶向筛查、代谢组学、分子网络等新兴分析策略,能够帮助检测实验室面对日益增长的食品安全需求。同时在数据处理、统计分析和化学物质鉴别方面,支持HRMS数据的软件和数据库也在不断地开发和扩展。文章从分析策略、化学计量学分析、数据库检索三个方面,简述了HRMS在食品质量及安全检测中的潜能和技术路线,系统阐述HRMS及相关分析技术在食品检测领域的发展趋势。展开更多
基金funded by China Scholarship Council(Grant No.202206180007)funded by China Scholarship Council(Grant No.202206180006).
文摘Background:This study aimed to develop a comprehensive instrument for evaluating and ranking clinical practice guidelines,named Scientific,Transparent and Applicable Rankings tool(STAR),and test its reliability,validity,and usability.Methods:This study set up a multidisciplinary working group including guideline methodologists,statisticians,journal editors,clinicians,and other experts.Scoping review,Delphi methods,and hierarchical analysis were used to develop the STAR tool.We evaluated the instrument’s intrinsic and interrater reliability,content and criterion validity,and usability.Results:STAR contained 39 items grouped into 11 domains.The mean intrinsic reliability of the domains,indicated by Cronbach’sαcoefficient,was 0.588(95%confidence interval[CI]:0.414,0.762).Interrater reliability as assessed with Cohen’s kappa coefficient was 0.774(95%CI:0.740,0.807)for methodological evaluators and 0.618(95%CI:0.587,0.648)for clinical evaluators.The overall content validity index was 0.905.Pearson’s r correlation for criterion validity was 0.885(95%CI:0.804,0.932).The mean usability score of the items was 4.6 and the median time spent to evaluate each guideline was 20 min.Conclusion:The instrument performed well in terms of reliability,validity,and efficiency,and can be used for comprehensively evaluating and ranking guidelines.
文摘目的研发指南解读类文献的报告规范,以期更好地帮助指南解读者报告指南重要信息。方法RIGHT for INT课题组参考EQUATOR网站推荐的报告规范制订方法,确定了3个阶段的制订流程:第一阶段,通过邮件和微信的途径招募多学科的专家,根据职能划分成核心制订小组、共识专家组及秘书组3个小组;第二阶段,采用文献调研和头脑风暴的方法收集初始条目;第三阶段,通过德尔菲调查和专家意见咨询,形成最终的RIGHT for INT清单。结果通过核心小组头脑风暴讨论和文献回顾,共收集40个初始条目。经过德尔菲调查和专家意见咨询过程(两轮均为17位专家,回复率均为100%),最终形成27个条目的RIGHT for INT清单。结论RIGHT for INT报告清单为指南解读者提供了撰写指南解读类文章的详细指导。
文摘目的了解沙盘游戏疗法对少年肾病综合征患儿心理特征、社交焦虑及生活质量的影响。方法采用便利抽样方法抽取武汉市某三级甲等医院2018年5月-2021年5月收治的700例少年肾病综合征患儿为研究对象,将其随机分为观察组和对照组,每组350例,其中对照组给予常规干预(即入院时介绍院内相关情况及疾病注意事项),观察组在对照组基础上实施沙盘游戏疗法。观察两组患儿干预前后应用Conners父母症状问卷(Parent Symptom Questionnaire,PSQ)、儿少心理健康量表(Mental Health Scale for Child and Adolescent,MHS-CA)、儿童社交焦虑量表(Social Anxiety Scale for Children,SASC)、儿少主观生活质量问卷(Inventory of Subjective Life Quality,ISLQ)评分。结果观察组干预后PSQ评分中各维度评分均明显低于对照组(P<0.05,Cohen's d>0.8),观察组干预后MHS-CA评分中各维度评分均明显高于对照组(P<0.05,Cohen's d>0.8),观察组干预后SASC评分中各维度评分均明显低于对照组(P<0.05,Cohen's d>0.8),观察组干预后ISLQ评分中各维度评分均明显高于对照组(P<0.05,Cohen's d>0.8)。结论沙盘游戏疗法能明显改善少年肾病综合征患儿的心理健康程度,缓解其社交焦虑,提升日常生活质量。
基金The Shenzhen Municipal Science and Technology Innovation Committee Project(JCYJ20190806160407178,JCYJ20180305164544288,JSGG20180504170427135,SGLH20180625141602256,JCYJ20180305164659637)the Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration(ZDSYS20200811143752005)supported this work。
文摘Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis.
基金Supported by the National Natural Science Foundation of China(82222062)the Jiangsu Provincial Science Fund for Distinguished Young Scholars(BK20220064).
文摘Summary What is already known about this topic?The quadrivalent influenza vaccine(QIV)provides protection against a broader range of influenza strains by including strains of influenza A/H1N1,A/H3N2,B/Yamagata,and B/Victoria.What is added by this report?This study aimed to assess the immunogenicity and safety of administering a single dose compared to two doses of QIV in children,taking into consideration their previous influenza vaccination history.
文摘高分辨质谱技术(High resolution mass spectrometer,HRMS)是具有食品安全风险识别能力的分析技术,理论上能够检测和鉴别食品中所有风险物质,适用于多种微量危害因子的鉴别和监测。基于HRMS的靶向高通量检测、非靶向筛查、代谢组学、分子网络等新兴分析策略,能够帮助检测实验室面对日益增长的食品安全需求。同时在数据处理、统计分析和化学物质鉴别方面,支持HRMS数据的软件和数据库也在不断地开发和扩展。文章从分析策略、化学计量学分析、数据库检索三个方面,简述了HRMS在食品质量及安全检测中的潜能和技术路线,系统阐述HRMS及相关分析技术在食品检测领域的发展趋势。
