AIM: To study the effects of transmitters ET, AgII, PGI2,CGRP and GG on experimental rat hepatic fibrosis and the antifibrogenic effects of IL-10.METHODS: One hundred SD rats were randomly divided into 3 groups: contr...AIM: To study the effects of transmitters ET, AgII, PGI2,CGRP and GG on experimental rat hepatic fibrosis and the antifibrogenic effects of IL-10.METHODS: One hundred SD rats were randomly divided into 3 groups: control group (N): intraperitoneal injection a week; IL-10 treated group (E): besides same dosage of the third week. In the fifth, the seventh and the ninth week,rats in three groups were selected randomly to collect plasma and liver tissues. The levels of ET, AgII, PGI2, CGRP and GG were assayed by radioimmunoassay (RIA). The liver fibrosis was observed with silver staining.RESULTS: The hepatic fibrosis was developed with the increase of the injection frequency of CCl4. The ET, AgII, PGI2, CGRP The histological examination showed that the degrees of the rats liver fibrosis in group E were lower than those in group C.CONCLUSION: The transmitters ET, AgII, PGI2, CGRP and GG play a significant role in the rat hepatic fibrosis induced by CCl4. IL-10 has the antagonistic action on these transmitters and can relieve the degree of the liver fibrosis.展开更多
AIM: To study the expression of IGF-1 and IGF-1R and its intervention by interleuldn-10 in the course of experimental hepatic fibrosis.METHODS: Hepatic fibrosis was induced in rats by carbon tetrachloride intoxication...AIM: To study the expression of IGF-1 and IGF-1R and its intervention by interleuldn-10 in the course of experimental hepatic fibrosis.METHODS: Hepatic fibrosis was induced in rats by carbon tetrachloride intoxication and liver specimens were taken from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group.Immunoreactivities for insulin-like growth factor-1 (IGF-1)and IGF-1 receptor(IGF-1R) were demonstrated by immunohistochemistry, and their intensities were evaluated in different animal groups.RESULTS: The positive levels for IGF-1 and IGF-1R were increased with the development of hepatic fibrosis, with the positive signals localized in cytoplasm and/or at the plasmic membrane of hepatocytes. The positive signals of IGF-1 and IGF-1R were observed more frequently (P<0.01) in the CCl4-treated group (92.0 % and 90.0 %) compared to those in the control group. The positive signals decreased significantly (P<0.05) in IL-10-treated group. The responses in IGF-1 and IGF-1R expression correlated with the time of IL-10 treatment.CONCLUSION: The expression of IGF-1 and IGF-1R immunoreactivities in liver tissue seems to be up-regulated during development of hepatic fibrosis induced by CC14, and exogenic IL-10 inhibits the responses.展开更多
剥隘鸡是云南省富宁县特有的地方小型家禽品种,以个小肉厚、微黄鲜嫩而得名。本试验采用高效液相色谱法(high performance liquid chromatography,HPLC)分别对不同性别的7月龄云南剥隘鸡胸肌和腿肌中的17种氨基酸进行测定分析。结果表明...剥隘鸡是云南省富宁县特有的地方小型家禽品种,以个小肉厚、微黄鲜嫩而得名。本试验采用高效液相色谱法(high performance liquid chromatography,HPLC)分别对不同性别的7月龄云南剥隘鸡胸肌和腿肌中的17种氨基酸进行测定分析。结果表明:剥隘鸡胸肌和腿肌(干重)中总氨基酸含量在65%~70%之间,其中胸肌氨基酸总量和必需氨基酸含量高于腿肌,公鸡肌肉氨基酸总量高于母鸡;肌肉鲜味氨基酸中以谷氨酸含量最高,天冬氨酸、精氨酸含量次之;肌肉中7种人体必需氨基酸种类齐全、含量丰富,必需氨基酸/总氨基酸(essential amino acids/total amino acids,EAA/TAA)和必需氨基酸/非必需氨基酸(essential amino acids/nonessential amino acids,EAA/NEAA)分别接近和高于世界卫生组织/联合国粮农组织(World Health Organization/Food and Agriculture Organization of the United Nations,WHO/FAO)的推荐值。试验结果为云南剥隘鸡肌肉的营养价值评定和进一步开发利用提供参考。展开更多
基金Natural Science Foundation of Fujian Province,No.C96042
文摘AIM: To study the effects of transmitters ET, AgII, PGI2,CGRP and GG on experimental rat hepatic fibrosis and the antifibrogenic effects of IL-10.METHODS: One hundred SD rats were randomly divided into 3 groups: control group (N): intraperitoneal injection a week; IL-10 treated group (E): besides same dosage of the third week. In the fifth, the seventh and the ninth week,rats in three groups were selected randomly to collect plasma and liver tissues. The levels of ET, AgII, PGI2, CGRP and GG were assayed by radioimmunoassay (RIA). The liver fibrosis was observed with silver staining.RESULTS: The hepatic fibrosis was developed with the increase of the injection frequency of CCl4. The ET, AgII, PGI2, CGRP The histological examination showed that the degrees of the rats liver fibrosis in group E were lower than those in group C.CONCLUSION: The transmitters ET, AgII, PGI2, CGRP and GG play a significant role in the rat hepatic fibrosis induced by CCl4. IL-10 has the antagonistic action on these transmitters and can relieve the degree of the liver fibrosis.
基金Science and Technology fund of Fujian Province,No.2003D05
文摘AIM: To study the expression of IGF-1 and IGF-1R and its intervention by interleuldn-10 in the course of experimental hepatic fibrosis.METHODS: Hepatic fibrosis was induced in rats by carbon tetrachloride intoxication and liver specimens were taken from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group.Immunoreactivities for insulin-like growth factor-1 (IGF-1)and IGF-1 receptor(IGF-1R) were demonstrated by immunohistochemistry, and their intensities were evaluated in different animal groups.RESULTS: The positive levels for IGF-1 and IGF-1R were increased with the development of hepatic fibrosis, with the positive signals localized in cytoplasm and/or at the plasmic membrane of hepatocytes. The positive signals of IGF-1 and IGF-1R were observed more frequently (P<0.01) in the CCl4-treated group (92.0 % and 90.0 %) compared to those in the control group. The positive signals decreased significantly (P<0.05) in IL-10-treated group. The responses in IGF-1 and IGF-1R expression correlated with the time of IL-10 treatment.CONCLUSION: The expression of IGF-1 and IGF-1R immunoreactivities in liver tissue seems to be up-regulated during development of hepatic fibrosis induced by CC14, and exogenic IL-10 inhibits the responses.
文摘剥隘鸡是云南省富宁县特有的地方小型家禽品种,以个小肉厚、微黄鲜嫩而得名。本试验采用高效液相色谱法(high performance liquid chromatography,HPLC)分别对不同性别的7月龄云南剥隘鸡胸肌和腿肌中的17种氨基酸进行测定分析。结果表明:剥隘鸡胸肌和腿肌(干重)中总氨基酸含量在65%~70%之间,其中胸肌氨基酸总量和必需氨基酸含量高于腿肌,公鸡肌肉氨基酸总量高于母鸡;肌肉鲜味氨基酸中以谷氨酸含量最高,天冬氨酸、精氨酸含量次之;肌肉中7种人体必需氨基酸种类齐全、含量丰富,必需氨基酸/总氨基酸(essential amino acids/total amino acids,EAA/TAA)和必需氨基酸/非必需氨基酸(essential amino acids/nonessential amino acids,EAA/NEAA)分别接近和高于世界卫生组织/联合国粮农组织(World Health Organization/Food and Agriculture Organization of the United Nations,WHO/FAO)的推荐值。试验结果为云南剥隘鸡肌肉的营养价值评定和进一步开发利用提供参考。
