Alternative sources of fish oil(FO)are one of the major problems in aquaculture;therefore,the goal of the present study was to examine insect(black soldier fly larvae)oil(BSLO)as a potential replacer of fish/s oy oil ...Alternative sources of fish oil(FO)are one of the major problems in aquaculture;therefore,the goal of the present study was to examine insect(black soldier fly larvae)oil(BSLO)as a potential replacer of fish/s oy oil in juvenile rainbow trout(initial average weight of 32±0.15 g)feed.Four diets were formulated wherein FO(control diet)was completely replaced with either soybean oil(SO)or BSLO,and an additional BSLO-based diet supplemented with 1.5%bile acid(BSLO+BA)were fed to the fish for 10 weeks.Growth performance of the BSLO fed group was similar(P>0.05)to that of the FO and SO fed groups,howeve r,the fish fed BSLO+BA diet registered the lowest growth(P<0.05).Oil sources did not(P>0.05)affect the major nutrient content of whole-body,however,the fatty acid composition of the muscle and liver was influenced(P<0.05),with the highest 14:0,16:0,and total saturated fatty acid detected in BSLO or BSLO+BA fed trout compared to the others(P<0.001).No significant diffe rences were observed in eicosapentaenoic acid+docosahexaenoic acid(EPA+DHA)or total n-3 polyunsaturated fatty acid(PUFA)content in muscle among the groups,whereas,the highest EPA:DHA and n-3:n-6 ratios were detected in the FO group.Gene expression for fatty acid binding protein(fabp),fatty acid synthase(fas),and△5 desaturase in the liver was lower in FO(P<0.05),while BSLO+BA registered the highest△6 expression(P=0.006).Supplementation of BA in the BSLO diet increased superoxide dismutase(SOD)and catalase(CAT)activities compared to the other groups(P<0.05).In conclusion,BSLO could serve as a substitute for FO and SO in rainbow trout diet without negatively impacting growth performance,whole-body composition and nutrient retention,and modulate the expression of fatty acid metabolism-related genes in rainbow trout.展开更多
基金the J.William Fulbright Scholarship Program for financial support as a Visiting Scholar at Aquaculture Research Institute,University of Idaho,USAthe United States Department of Agriculture Agricultural Research Service for partially funding the research(Accession numbers:437676&436723)。
文摘Alternative sources of fish oil(FO)are one of the major problems in aquaculture;therefore,the goal of the present study was to examine insect(black soldier fly larvae)oil(BSLO)as a potential replacer of fish/s oy oil in juvenile rainbow trout(initial average weight of 32±0.15 g)feed.Four diets were formulated wherein FO(control diet)was completely replaced with either soybean oil(SO)or BSLO,and an additional BSLO-based diet supplemented with 1.5%bile acid(BSLO+BA)were fed to the fish for 10 weeks.Growth performance of the BSLO fed group was similar(P>0.05)to that of the FO and SO fed groups,howeve r,the fish fed BSLO+BA diet registered the lowest growth(P<0.05).Oil sources did not(P>0.05)affect the major nutrient content of whole-body,however,the fatty acid composition of the muscle and liver was influenced(P<0.05),with the highest 14:0,16:0,and total saturated fatty acid detected in BSLO or BSLO+BA fed trout compared to the others(P<0.001).No significant diffe rences were observed in eicosapentaenoic acid+docosahexaenoic acid(EPA+DHA)or total n-3 polyunsaturated fatty acid(PUFA)content in muscle among the groups,whereas,the highest EPA:DHA and n-3:n-6 ratios were detected in the FO group.Gene expression for fatty acid binding protein(fabp),fatty acid synthase(fas),and△5 desaturase in the liver was lower in FO(P<0.05),while BSLO+BA registered the highest△6 expression(P=0.006).Supplementation of BA in the BSLO diet increased superoxide dismutase(SOD)and catalase(CAT)activities compared to the other groups(P<0.05).In conclusion,BSLO could serve as a substitute for FO and SO in rainbow trout diet without negatively impacting growth performance,whole-body composition and nutrient retention,and modulate the expression of fatty acid metabolism-related genes in rainbow trout.
