黄酒酒糟是黄酒生产得到的副产物,经过第一次发酵后得到。发酵后的酒糟中蛋白质含量高达26.7%、淀粉含量高达29.96%,为使酒糟得到进一步的利用,本实验将甜酒糟进行二次发酵处理并进行优化,经过优化后得到风味物质提高效果优异,当加水量...黄酒酒糟是黄酒生产得到的副产物,经过第一次发酵后得到。发酵后的酒糟中蛋白质含量高达26.7%、淀粉含量高达29.96%,为使酒糟得到进一步的利用,本实验将甜酒糟进行二次发酵处理并进行优化,经过优化后得到风味物质提高效果优异,当加水量为65 m L、加酶量为1%、加菌量为10%时,发酵得到风味物质最多,为3220.14 mg/L。展开更多
The Gaoligong Mountains(GLGM),located in southwestern China,extend north to south along the western border of the Hengduan Mountains,spanning approximately 600 km.In this study,we consolidated findings from 17 bird su...The Gaoligong Mountains(GLGM),located in southwestern China,extend north to south along the western border of the Hengduan Mountains,spanning approximately 600 km.In this study,we consolidated findings from 17 bird surveys conducted in the GLGM between 2010 and 2022.We found that the GLGM harbors tremendous bird diversity,with a total of 796 documented bird species in the region.Nearly a quarter(23.0%)of these species are listed as state key protected species or as Chinese and global threatened species.Analysis of species richness at the county level showed a decreasing trend with increasing latitude,with the greatest diversity in Yingjiang(661 species).Observations indicated that the GLGM belongs to the Oriental realm,primarily composed of bird species from southern and southwestern China.The GLGM plays an important role in avian conservation by sheltering exceptional bird diversity,providing corridors and flyways for bird migration and dispersal,and mitigating the effects of climate change.In response to the conservation needs of birds and other wildlife,the Chinese government has established numerous protected areas within the GLGM.Despite these efforts,avian conservation still faces considerable challenges in the GLGM due to limitations in the protected area network,transboundary nature of the regions,and existing gaps in monitoring and research.展开更多
Background： Accumulating documents have demonstrated that long noncoding RNAs （lncRNAs） play critical roles in tumorigenesis. As an lncRNA, nuclear-enriched abundant transcript 1 （NEAT1） has been identified to be...Background： Accumulating documents have demonstrated that long noncoding RNAs （lncRNAs） play critical roles in tumorigenesis. As an lncRNA, nuclear-enriched abundant transcript 1 （NEAT1） has been identified to be involved in the progression of many types of cancers. However, the biological function of NE.4T1 in cervical cancer is not fully investigated. The aim of this study was to disclose the specific biological function of lncRNA NEATI in cervical cancer progression. Methods： Quantitative real-time polymerase chain reaction （qRT-PCR） was utilized to identify the expression of lncRNA NE,4 T1 in the cervical cancer tissues and cell lines. All cervical cancer samples used in this study were collected from the Affiliated Suzhou Hospital of Nanjing Medical University between September 2012 and September 2017. The correlation between NE,4T1 expression and the overall survival rate of cervical cancer patients was analyzed by Kaplan-Meier analysis. The effects of NEAT1 knockdown or overexpression on cell proliferation were tested by performing MTT assays and colony formation assays. Transwell assays were conducted to detect the migratory ability of cervical cancer cells, in which NEAT1 was silenced or overexpressed. Western blotting was utilized to validate whether NEAT1 promotes cervical cancer progression through activating PI3K-Akt signaling pathway. Results： High expression of NE,4T1 predicted poor prognosis of cervical cancer patients （χ^2= 0.735, P = 0.005）. Knockdown of NE,4T1 decreased the number of colonies in CaSki cell from 136.667 ± 13.503 to 71.667 ± 7.506 （t = -18.76, P = 0.003） and decreased the number of colonies in HeLa cell from 128.667 ± 13.317 to 65.667 ± 7.024 （t = -5.54, P = 0.031）. However, overexpression of NEA T1 increased the number of colonies in SiHa cell from 84.667 ± 12.014 to 150.667 ± 18.037 （t = 7.27, P = 0.018）. Knockdown of NEAT1 decreased the migratory number of CaSki cell from 100.333 ± 9.866 to 58.333 ± 5.859 （t = -8.08, P = 0.015） an展开更多
Objective： To explore whether single and fused monochromatic images can improve liver tumor detection and delineation by single source dual energy CT （ssDECT） in patients with hepatocellular carcinoma （HCC） durin...Objective： To explore whether single and fused monochromatic images can improve liver tumor detection and delineation by single source dual energy CT （ssDECT） in patients with hepatocellular carcinoma （HCC） during arterial phase. Methods： Fifty-seven patients with HCC who underwent ssDECT scanning at Beijing Cancer Hospital were enrolled retrospectively. Twenty-one sets of monochromatic images from 40 to 140 keV were reconstructed at 5 keV intervals in arterial phase. The optimal contrast-noise ratio （CNR） monochromatic images of the liver tumor and the lowest-noise monochromatic images were selected for image fusion. We evaluated the image quality of the optimal-CNR monochromatic images, the lowest-noise monochromatic images and the fused monochromatic images, respectively. The evaluation indicators included the spatial resolution of the anatomical structure, the noise level, the contrast and CNR of the tumor. Results： In arterial phase, the anatomical structure of the liver can be displayed most clearly in the 65-keV monochromatic images, with the lowest image noise. The optimal-CNR monochromatic images of HCC tumor were 50-keV monochromatic images in which the internal structural features of the liver tumors were displayed most clearly and meticulously. For tumor detection, the fused monochromatic images and the 50-keV monochromatic images had similar performances, and were more sensitive than 65-keV monochromatic images. Conclusions： We achieved good arterial phase images by fusing the optimal-CNR monochromatic images of the HCC tumor and the lowest-noise monochromatic images. The fused images displayed liver tumors and anatomical structures more clearly, which is potentially helpful for identifying more and smaller HCC tumors.展开更多
As a naturally occurring cytolytic peptide,melittin(Mel)has strong cytolytic activity and is a potent therapeutic peptide for cancer therapy.However,the serious hemolytic activity of Mel largely impedes its clinical a...As a naturally occurring cytolytic peptide,melittin(Mel)has strong cytolytic activity and is a potent therapeutic peptide for cancer therapy.However,the serious hemolytic activity of Mel largely impedes its clinical applications.In this work,based on the strong interactions between proteins/peptides and polyphenols,we develop a tannic acid–Fe^(3+)metal–phenolic network(MPN)-based strategy that can convert Mel from foe to friend via shielding its positive charges and reducing its hemolytic activity.Besides,an immune adjuvant resiquimod(R848)is also introduced for immunostimulation,affording the final Mel-and R848-coloaded nanodrug.The Mel-caused membrane disruption can induce immunogenic cell death for immunostimulation,R848 can act as an immune adjuvant to further facilitate the immunostimulatory effect,and the tannic acid–Fe^(3+)MPN-mediated Fenton reaction can produce reactive oxygen species for cancer treatment.Further experiments reveal that the nanodrug can effectively cause immunogenic cell death of tumor cells and arouse robust intratumoral and systemic antitumor immunostimulation.In the bilateral tumor-bearing mouse models,the nanodrug considerably destroys the primary tumor and also boosts the abscopal effect to ablate the distant tumor.Collectively,the MPN-facilitated“foe-to-friend”strategy may promote the practical applications of Mel and foster the development of cancer immunotherapeutics.展开更多
In Ga N light-emitting diodes（LEDs） with Ga N/In Ga N/Al Ga N/In Ga N/Ga N composition-graded barriers are proposed to replace the sixth and the middle five Ga N barriers under the condition of removing the electron...In Ga N light-emitting diodes（LEDs） with Ga N/In Ga N/Al Ga N/In Ga N/Ga N composition-graded barriers are proposed to replace the sixth and the middle five Ga N barriers under the condition of removing the electron blocking layer（EBL）and studied numerically in this paper. Simulation results show that the specially designed barrier in the sixth barrier is able to modulate the distributions of the holes and electrons in quantum well which is adjacent to the specially designed barrier. Concretely speaking, the new barrier could enhance both the electron and hole concentration remarkably in the previous well and reduce the hole concentration for the latter one to some extent along the growth direction. What is more,a phenomenon, i.e., a better carrier distribution in all the wells, just appears with the adoption of the new barriers in the middle five barriers, resulting in a much higher light output power and a lower efficiency droop than those in a conventional LED structure.展开更多
This paper simulated the optimal refrigerant charge inventory of a refrigeration system in air-conditioning operation and heat-pump operation respectively,and studied the refrigerant control strategies in this system....This paper simulated the optimal refrigerant charge inventory of a refrigeration system in air-conditioning operation and heat-pump operation respectively,and studied the refrigerant control strategies in this system.The void fraction in two-phase fluid region was calculated by Harms model.And based on distributed parameter model and Harms model,the refrigerant charge inventory in condenser and evaporator were calculated and analyzed in air-conditioning conditions and heat-pump conditions,respectively.The calculating results of different refrigerant mass between refrigeration and heating conditions indicate that the optimal refrigerant charge inventory in heat-pump conditions is lower than that in air-conditioning conditions.To avoid the decrease of COP due to the surplus refrigerant in heating conditions,we introduced the liquid reservoir control method and associate capillary control method.Both of them could increase the heating capacity of the air-source heat pump.The difference of optimal refrigerant charge inventory in air-conditioning and heat-pump system can be controlled by the liquid reservoir or the associate capillary.展开更多
We present high spatial resolution X-ray spectroscopy of supernova rem- nant G292.0＋1.8 made with Chandra observations. The X-ray emitting region of this remnant was divided into 25 × 25 pixels with a scale of 2...We present high spatial resolution X-ray spectroscopy of supernova rem- nant G292.0＋1.8 made with Chandra observations. The X-ray emitting region of this remnant was divided into 25 × 25 pixels with a scale of 20＂ × 20＂ each. Spectra of 328 pixels were created and fitted with an absorbed one component non-equilibrium ionization model. With the spectral analysis results, we obtained maps of absorbing column density, temperature, ionization age and abundances for O, Ne, Mg, Si, S and Fe. The abundances of O, Ne and Mg show tight correlations between each other in the range of about two orders of magnitude, suggesting that they are all from explo- sive C/Ne burning. Meanwhile, the abundances of Si and S are also well correlated, indicating that they are the ashes of explosive O-burning or incomplete Si-burning. The Fe emission lines are not prominent in the whole remnant, and their abundance is significantly reduced, indicating that the reverse shock may not have propagated to the Fe-rich ejecta. Based on relative abundances of O, Ne, Mg, Si and Fe to Si, we suggest a progenitor mass of 25 - 30 M⊙ for this remnant.展开更多
文摘黄酒酒糟是黄酒生产得到的副产物,经过第一次发酵后得到。发酵后的酒糟中蛋白质含量高达26.7%、淀粉含量高达29.96%,为使酒糟得到进一步的利用,本实验将甜酒糟进行二次发酵处理并进行优化,经过优化后得到风味物质提高效果优异,当加水量为65 m L、加酶量为1%、加菌量为10%时,发酵得到风味物质最多,为3220.14 mg/L。
基金supported by the National Key R&D Program of China(2022YFC2602500,2022YFC2602502)Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment,China,Second Xizang Plateau Scientific Expedition and Research Program(STEP,2019QZKK0501)+3 种基金Major Science and Technique Programs in Yunnan Province(202102AA310055)National Natural Science Foundation of China(32070435)Science and Technology Basic Resources Investigation Program of China“Wild germplasm collection and preservation in Great Gaoligong Mountain”(2021FY100200)Project for Talent and Platform of Science and Technology in Yunnan Province Science and Technology Department(202205AM070007)。
文摘The Gaoligong Mountains(GLGM),located in southwestern China,extend north to south along the western border of the Hengduan Mountains,spanning approximately 600 km.In this study,we consolidated findings from 17 bird surveys conducted in the GLGM between 2010 and 2022.We found that the GLGM harbors tremendous bird diversity,with a total of 796 documented bird species in the region.Nearly a quarter(23.0%)of these species are listed as state key protected species or as Chinese and global threatened species.Analysis of species richness at the county level showed a decreasing trend with increasing latitude,with the greatest diversity in Yingjiang(661 species).Observations indicated that the GLGM belongs to the Oriental realm,primarily composed of bird species from southern and southwestern China.The GLGM plays an important role in avian conservation by sheltering exceptional bird diversity,providing corridors and flyways for bird migration and dispersal,and mitigating the effects of climate change.In response to the conservation needs of birds and other wildlife,the Chinese government has established numerous protected areas within the GLGM.Despite these efforts,avian conservation still faces considerable challenges in the GLGM due to limitations in the protected area network,transboundary nature of the regions,and existing gaps in monitoring and research.
基金This study was supported by grants from the National Natural Science Foundation of China （No. 81370719 and No. 81671535）, the Science Foundation of Jiangsu （No. BE2015642, and No. WSW023）, the Jiangsu Key Discipline of Human Assisted Reproduction Medicine Foundation （No. FXK201749）, and the Jiangsu Provincial Medical Youth Talent of the Project of Invigorating Health Care through Science, Technology, and Education （No. ZDRCA2016044）.
文摘Background： Accumulating documents have demonstrated that long noncoding RNAs （lncRNAs） play critical roles in tumorigenesis. As an lncRNA, nuclear-enriched abundant transcript 1 （NEAT1） has been identified to be involved in the progression of many types of cancers. However, the biological function of NE.4T1 in cervical cancer is not fully investigated. The aim of this study was to disclose the specific biological function of lncRNA NEATI in cervical cancer progression. Methods： Quantitative real-time polymerase chain reaction （qRT-PCR） was utilized to identify the expression of lncRNA NE,4 T1 in the cervical cancer tissues and cell lines. All cervical cancer samples used in this study were collected from the Affiliated Suzhou Hospital of Nanjing Medical University between September 2012 and September 2017. The correlation between NE,4T1 expression and the overall survival rate of cervical cancer patients was analyzed by Kaplan-Meier analysis. The effects of NEAT1 knockdown or overexpression on cell proliferation were tested by performing MTT assays and colony formation assays. Transwell assays were conducted to detect the migratory ability of cervical cancer cells, in which NEAT1 was silenced or overexpressed. Western blotting was utilized to validate whether NEAT1 promotes cervical cancer progression through activating PI3K-Akt signaling pathway. Results： High expression of NE,4T1 predicted poor prognosis of cervical cancer patients （χ^2= 0.735, P = 0.005）. Knockdown of NE,4T1 decreased the number of colonies in CaSki cell from 136.667 ± 13.503 to 71.667 ± 7.506 （t = -18.76, P = 0.003） and decreased the number of colonies in HeLa cell from 128.667 ± 13.317 to 65.667 ± 7.024 （t = -5.54, P = 0.031）. However, overexpression of NEA T1 increased the number of colonies in SiHa cell from 84.667 ± 12.014 to 150.667 ± 18.037 （t = 7.27, P = 0.018）. Knockdown of NEAT1 decreased the migratory number of CaSki cell from 100.333 ± 9.866 to 58.333 ± 5.859 （t = -8.08, P = 0.015） an
基金supported by the National Basic Research Program of China (973 Program) (Grant No. 2011CB707705)National Natural Science Foundation of China (Grant No. 81371715+1 种基金 81201215)the Capital Characteristic Clinical Application Research (Grant No. Z121107001012115)
文摘Objective： To explore whether single and fused monochromatic images can improve liver tumor detection and delineation by single source dual energy CT （ssDECT） in patients with hepatocellular carcinoma （HCC） during arterial phase. Methods： Fifty-seven patients with HCC who underwent ssDECT scanning at Beijing Cancer Hospital were enrolled retrospectively. Twenty-one sets of monochromatic images from 40 to 140 keV were reconstructed at 5 keV intervals in arterial phase. The optimal contrast-noise ratio （CNR） monochromatic images of the liver tumor and the lowest-noise monochromatic images were selected for image fusion. We evaluated the image quality of the optimal-CNR monochromatic images, the lowest-noise monochromatic images and the fused monochromatic images, respectively. The evaluation indicators included the spatial resolution of the anatomical structure, the noise level, the contrast and CNR of the tumor. Results： In arterial phase, the anatomical structure of the liver can be displayed most clearly in the 65-keV monochromatic images, with the lowest image noise. The optimal-CNR monochromatic images of HCC tumor were 50-keV monochromatic images in which the internal structural features of the liver tumors were displayed most clearly and meticulously. For tumor detection, the fused monochromatic images and the 50-keV monochromatic images had similar performances, and were more sensitive than 65-keV monochromatic images. Conclusions： We achieved good arterial phase images by fusing the optimal-CNR monochromatic images of the HCC tumor and the lowest-noise monochromatic images. The fused images displayed liver tumors and anatomical structures more clearly, which is potentially helpful for identifying more and smaller HCC tumors.
基金the Natural Science Foundation of Jiangsu Province(BK20211510)the National Natural Science Foundation of China(21673037)。
文摘As a naturally occurring cytolytic peptide,melittin(Mel)has strong cytolytic activity and is a potent therapeutic peptide for cancer therapy.However,the serious hemolytic activity of Mel largely impedes its clinical applications.In this work,based on the strong interactions between proteins/peptides and polyphenols,we develop a tannic acid–Fe^(3+)metal–phenolic network(MPN)-based strategy that can convert Mel from foe to friend via shielding its positive charges and reducing its hemolytic activity.Besides,an immune adjuvant resiquimod(R848)is also introduced for immunostimulation,affording the final Mel-and R848-coloaded nanodrug.The Mel-caused membrane disruption can induce immunogenic cell death for immunostimulation,R848 can act as an immune adjuvant to further facilitate the immunostimulatory effect,and the tannic acid–Fe^(3+)MPN-mediated Fenton reaction can produce reactive oxygen species for cancer treatment.Further experiments reveal that the nanodrug can effectively cause immunogenic cell death of tumor cells and arouse robust intratumoral and systemic antitumor immunostimulation.In the bilateral tumor-bearing mouse models,the nanodrug considerably destroys the primary tumor and also boosts the abscopal effect to ablate the distant tumor.Collectively,the MPN-facilitated“foe-to-friend”strategy may promote the practical applications of Mel and foster the development of cancer immunotherapeutics.
基金Project supported by the Science and Technology Program Project for the Innovation of Forefront and Key Technology of Guangdong Province,China(Grant No.2014B010121001)the Special Funds for Strategic Emerging Industries of Guangdong Province,China(Grant No.2012A080304006)+3 种基金the Special Funds for the Innovation of Forefront and Key Technology of Guangdong Province,China(Grant No.2014B010119004)the Science and Technology Program Project for High Conversion Efficiency and Application of Direct Driver High-end LED Chip of Guangdong Province,China(Grant No.2013B010204065)the Special Project for Key Science and Technology of Zhongshan City,Guangdong Province,China(Grant No.2014A2FC204)the Science and Technology Program Project in Huadu District of Guangzhou City,China(Grant No.HD15PT003)
文摘In Ga N light-emitting diodes（LEDs） with Ga N/In Ga N/Al Ga N/In Ga N/Ga N composition-graded barriers are proposed to replace the sixth and the middle five Ga N barriers under the condition of removing the electron blocking layer（EBL）and studied numerically in this paper. Simulation results show that the specially designed barrier in the sixth barrier is able to modulate the distributions of the holes and electrons in quantum well which is adjacent to the specially designed barrier. Concretely speaking, the new barrier could enhance both the electron and hole concentration remarkably in the previous well and reduce the hole concentration for the latter one to some extent along the growth direction. What is more,a phenomenon, i.e., a better carrier distribution in all the wells, just appears with the adoption of the new barriers in the middle five barriers, resulting in a much higher light output power and a lower efficiency droop than those in a conventional LED structure.
基金Supported by Hubei Provincial Natural Science Foundation(2008CDB363)
文摘This paper simulated the optimal refrigerant charge inventory of a refrigeration system in air-conditioning operation and heat-pump operation respectively,and studied the refrigerant control strategies in this system.The void fraction in two-phase fluid region was calculated by Harms model.And based on distributed parameter model and Harms model,the refrigerant charge inventory in condenser and evaporator were calculated and analyzed in air-conditioning conditions and heat-pump conditions,respectively.The calculating results of different refrigerant mass between refrigeration and heating conditions indicate that the optimal refrigerant charge inventory in heat-pump conditions is lower than that in air-conditioning conditions.To avoid the decrease of COP due to the surplus refrigerant in heating conditions,we introduced the liquid reservoir control method and associate capillary control method.Both of them could increase the heating capacity of the air-source heat pump.The difference of optimal refrigerant charge inventory in air-conditioning and heat-pump system can be controlled by the liquid reservoir or the associate capillary.
基金Supported by the National Natural Science Foundation of China
文摘We present high spatial resolution X-ray spectroscopy of supernova rem- nant G292.0＋1.8 made with Chandra observations. The X-ray emitting region of this remnant was divided into 25 × 25 pixels with a scale of 20＂ × 20＂ each. Spectra of 328 pixels were created and fitted with an absorbed one component non-equilibrium ionization model. With the spectral analysis results, we obtained maps of absorbing column density, temperature, ionization age and abundances for O, Ne, Mg, Si, S and Fe. The abundances of O, Ne and Mg show tight correlations between each other in the range of about two orders of magnitude, suggesting that they are all from explo- sive C/Ne burning. Meanwhile, the abundances of Si and S are also well correlated, indicating that they are the ashes of explosive O-burning or incomplete Si-burning. The Fe emission lines are not prominent in the whole remnant, and their abundance is significantly reduced, indicating that the reverse shock may not have propagated to the Fe-rich ejecta. Based on relative abundances of O, Ne, Mg, Si and Fe to Si, we suggest a progenitor mass of 25 - 30 M⊙ for this remnant.
