采用GFS背景场资料和ADAS资料同化系统,使用WRF模式对2014—2016年青岛近海17个海雾个例进行了模拟,分析了3种能见度算法的预报效果。结果表明,FSL(Forecast Systems Laboratory)算法对于沿海站、岸基站雾的预报较SW99(Steolinga and Wa...采用GFS背景场资料和ADAS资料同化系统,使用WRF模式对2014—2016年青岛近海17个海雾个例进行了模拟,分析了3种能见度算法的预报效果。结果表明,FSL(Forecast Systems Laboratory)算法对于沿海站、岸基站雾的预报较SW99(Steolinga and Warner 1999)算法有优势;对于海岛站而言,SW99算法则优于FSL算法。混合算法CVIS(Combined Visibility)较单一算法预报雾准确率有所提高。3种能见度算法基本上是高估能见度的,SW99算法能见度预报均方根误差最大。另外,SW99算法对沿海站、岸基站雾开始时间预报较实况多偏晚,结束时间预报较实况多偏早,持续时间预报较实况多偏短。展开更多
Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals.However,the existence of bottlenecks in synthetic pathway ca...Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals.However,the existence of bottlenecks in synthetic pathway can seriously affect production efficiency,which is also one of the core issues for metabolic engineers to solve.Therefore,developing an approach for diagnosing potential metabolic bottlenecks in a faster and simpler manner is of great significance to accelerate cell factories construction.The cell-free reaction system based on cell lysates can transfer metabolic reactions from in vivo to in vitro,providing a flexible access to directly change protein and metabolite variables,thus provides a potential solution for rapid identification of bottlenecks.Here,bottleneck diagnosis of the N-acetylneuraminic acid(NeuAc)biosynthesis pathway in industrially important chassis microorganism Bacillus subtilis was performed using cell-free synthesis system.Specifically,a highly efficient B.subtilis cell-free system for NeuAc de novo synthesis was firstly constructed,which had a 305-fold NeuAc synthesis rate than that in vivo and enabled fast pathway dynamics analysis.Next,through the addition of all potential key intermediates in combination with substrate glucose respectively,it was found that insufficient phosphoenolpyruvate supply was one of the NeuAc pathway bottlenecks.Rational in vivo metabolic engineering of NeuAc-producing B.subtilis was further performed to eliminate the bottleneck.By down-regulating the expression level of pyruvate kinase throughout the growth phase or only in the stationary phase using inhibitory Nterminal coding sequences(NCSs)and growth-dependent regulatory NCSs respectively,the maximal NeuAc titer increased 2.0-fold.Our study provides a rapid method for bottleneck diagnosis,which may help to accelerate the cycle of design,build,test and learn cycle for metabolic engineering.展开更多
[Objectives] To study the therapeutic effect and mechanism of New Maixian Powder on ulcerative colitis( UC) rats through observing its regulatory effect on the protein kinase R-like endoplasmic reticulum kinase( PERK)...[Objectives] To study the therapeutic effect and mechanism of New Maixian Powder on ulcerative colitis( UC) rats through observing its regulatory effect on the protein kinase R-like endoplasmic reticulum kinase( PERK)/eukaryotic translation initiation factor-2α( e IF-2α)/nuclear transcription factor-kappa B( NF-κB) signaling pathway. [Methods]First,60 SD rats were randomly divided into normal group,model group,mesalazine group,and New Maixian Powder low,medium and high dose groups,10 rats each group. Then,dextran sulfate sodium( DSS) was used to induce UC rats. The mesalazine group was given 0. 42 g/( kg·d) of mesalazine sustained-release granule suspension,New Maixian Powder low,medium and high dose groups were given 1. 5,3,and 6 g/( kg·d) of New Maixian Powder suspension,respectively,and other groups were given an equal volume of physiological saline,continuous intragastric administration for 14 d. Next,the disease activity index( DAI) of UC rats was evaluated; the expression of NF-κB in serum was measured by enzyme-linked immunosorbent assay( ELISA); the expression of PERK and e IF-2α protein and m RNA in colon tissue was detected by Western blot and real-time quantitative polymerase chain reaction( RT q-PCR). [Results] Compared with the normal group,the DAI score and serum NF-κB level in the model group were significantly higher( P < 0. 05),and PERK and e IF-2α protein and m RNA levels in the colon tissue were increased( P < 0. 05); compared with the model group,the DAI score decreased and serum NF-κB level declined in the New Maixian Powder group,and the expression of PERK and e IF-2α protein and m RNA in New Maixian Powder medium dose and high dose groups declined( P < 0. 05). [Conclusions]New Maixian Powder has good therapeutic effect on UC rats,and its mechanism may be connected with the inhibition of the activation of PERK/e IF-2α/NF-κB signaling pathway.展开更多
基于2020年上半年我国东部近海站点观测资料和葵花-8卫星反演海雾产品对我国自主研发的GRAPES-TYM模式进行了海雾预报性能评估。点、面检验结果表明:模式48 h和72 h TS分别为0.40和0.36,黄海海雾预报性能最优,34°~37°N海域内...基于2020年上半年我国东部近海站点观测资料和葵花-8卫星反演海雾产品对我国自主研发的GRAPES-TYM模式进行了海雾预报性能评估。点、面检验结果表明:模式48 h和72 h TS分别为0.40和0.36,黄海海雾预报性能最优,34°~37°N海域内大部分站点TS高于0.50。黄海海雾落区预报检验显示均压场形势下预报最准确,平均临界成功指数为0.35;气旋后部海雾多空报。2 m相对湿度预报偏差具有局地性特征,相对湿度低估的站海雾预报击中率、TS相对低,反之亦然。另外,模式对成雾相关气象要素预报误差相对小且对成雾有利时海雾预报基本正确;模式预报风向较实际风向偏东南,易出现冷偏差和湿偏差,虚假的有利温湿条件导致海雾空报。展开更多
文摘采用GFS背景场资料和ADAS资料同化系统,使用WRF模式对2014—2016年青岛近海17个海雾个例进行了模拟,分析了3种能见度算法的预报效果。结果表明,FSL(Forecast Systems Laboratory)算法对于沿海站、岸基站雾的预报较SW99(Steolinga and Warner 1999)算法有优势;对于海岛站而言,SW99算法则优于FSL算法。混合算法CVIS(Combined Visibility)较单一算法预报雾准确率有所提高。3种能见度算法基本上是高估能见度的,SW99算法能见度预报均方根误差最大。另外,SW99算法对沿海站、岸基站雾开始时间预报较实况多偏晚,结束时间预报较实况多偏早,持续时间预报较实况多偏短。
基金This study is financially supported by the National Key Research and Development Program of China(2018YFA0900300)National Natural Science Foundation of China(31972854)+3 种基金Key Research and Development Program of Jiangsu Province(BE2019628)Fundamental Research Funds for the Central Universities(JUSRP52020A)National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-16)Postgraduate Research&Practice Innovation Program of Jiangsu Province(JNKY19_015).
文摘Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals.However,the existence of bottlenecks in synthetic pathway can seriously affect production efficiency,which is also one of the core issues for metabolic engineers to solve.Therefore,developing an approach for diagnosing potential metabolic bottlenecks in a faster and simpler manner is of great significance to accelerate cell factories construction.The cell-free reaction system based on cell lysates can transfer metabolic reactions from in vivo to in vitro,providing a flexible access to directly change protein and metabolite variables,thus provides a potential solution for rapid identification of bottlenecks.Here,bottleneck diagnosis of the N-acetylneuraminic acid(NeuAc)biosynthesis pathway in industrially important chassis microorganism Bacillus subtilis was performed using cell-free synthesis system.Specifically,a highly efficient B.subtilis cell-free system for NeuAc de novo synthesis was firstly constructed,which had a 305-fold NeuAc synthesis rate than that in vivo and enabled fast pathway dynamics analysis.Next,through the addition of all potential key intermediates in combination with substrate glucose respectively,it was found that insufficient phosphoenolpyruvate supply was one of the NeuAc pathway bottlenecks.Rational in vivo metabolic engineering of NeuAc-producing B.subtilis was further performed to eliminate the bottleneck.By down-regulating the expression level of pyruvate kinase throughout the growth phase or only in the stationary phase using inhibitory Nterminal coding sequences(NCSs)and growth-dependent regulatory NCSs respectively,the maximal NeuAc titer increased 2.0-fold.Our study provides a rapid method for bottleneck diagnosis,which may help to accelerate the cycle of design,build,test and learn cycle for metabolic engineering.
基金Supported by Science and Technology Program of Zhejiang Province(2016C33085)
文摘[Objectives] To study the therapeutic effect and mechanism of New Maixian Powder on ulcerative colitis( UC) rats through observing its regulatory effect on the protein kinase R-like endoplasmic reticulum kinase( PERK)/eukaryotic translation initiation factor-2α( e IF-2α)/nuclear transcription factor-kappa B( NF-κB) signaling pathway. [Methods]First,60 SD rats were randomly divided into normal group,model group,mesalazine group,and New Maixian Powder low,medium and high dose groups,10 rats each group. Then,dextran sulfate sodium( DSS) was used to induce UC rats. The mesalazine group was given 0. 42 g/( kg·d) of mesalazine sustained-release granule suspension,New Maixian Powder low,medium and high dose groups were given 1. 5,3,and 6 g/( kg·d) of New Maixian Powder suspension,respectively,and other groups were given an equal volume of physiological saline,continuous intragastric administration for 14 d. Next,the disease activity index( DAI) of UC rats was evaluated; the expression of NF-κB in serum was measured by enzyme-linked immunosorbent assay( ELISA); the expression of PERK and e IF-2α protein and m RNA in colon tissue was detected by Western blot and real-time quantitative polymerase chain reaction( RT q-PCR). [Results] Compared with the normal group,the DAI score and serum NF-κB level in the model group were significantly higher( P < 0. 05),and PERK and e IF-2α protein and m RNA levels in the colon tissue were increased( P < 0. 05); compared with the model group,the DAI score decreased and serum NF-κB level declined in the New Maixian Powder group,and the expression of PERK and e IF-2α protein and m RNA in New Maixian Powder medium dose and high dose groups declined( P < 0. 05). [Conclusions]New Maixian Powder has good therapeutic effect on UC rats,and its mechanism may be connected with the inhibition of the activation of PERK/e IF-2α/NF-κB signaling pathway.
文摘基于2020年上半年我国东部近海站点观测资料和葵花-8卫星反演海雾产品对我国自主研发的GRAPES-TYM模式进行了海雾预报性能评估。点、面检验结果表明:模式48 h和72 h TS分别为0.40和0.36,黄海海雾预报性能最优,34°~37°N海域内大部分站点TS高于0.50。黄海海雾落区预报检验显示均压场形势下预报最准确,平均临界成功指数为0.35;气旋后部海雾多空报。2 m相对湿度预报偏差具有局地性特征,相对湿度低估的站海雾预报击中率、TS相对低,反之亦然。另外,模式对成雾相关气象要素预报误差相对小且对成雾有利时海雾预报基本正确;模式预报风向较实际风向偏东南,易出现冷偏差和湿偏差,虚假的有利温湿条件导致海雾空报。
