Carbon dots(CDs)with superior fluorescence properties have attracted a growing number of research interests in anti-counterfeiting.However,the preparation of CDs with thermally turn-on fluorescence and full-color-emit...Carbon dots(CDs)with superior fluorescence properties have attracted a growing number of research interests in anti-counterfeiting.However,the preparation of CDs with thermally turn-on fluorescence and full-color-emitting in visible spectrum is still a big challenge due to the complicated reaction mechanism in the formation of CDs.Here,a simple precursor-oriented strategy for the preparation of multicolor CDs with heat-stimuli turn-on fluorescence is reported.Comprehensive experimental characterizations and theoretical calculations revealed that the emission wavelength of CDs can be readily tuned from 460 nm to 654 nm with selected precursors,which was ascribed to the extent of conjugated sp2-domains(core states)and the amount of oxygen-and nitrogen-containing groups bound to sp2-domains(surface states).After simply mixing two or three kinds of CDs,a full-color range of fluorescence emission was realized,and the CDs-based fluorescence inks were successfully fabricated.Particularly,all the printed patterns from the inkjet exhibited a thermal-induced enhancement in fluorescence.On this basis,combining CDs with heating-induced“turn-off”fluorescence materials can lead to multidimensional and multistage encryption.These results demonstrate that the thermochromic and photochromic CDs with much more enhanced security exhibit promising application in data storage and encryption.展开更多
Owing to their limited accuracy and narrow applicability,current antimicrobial peptide(AMP)prediction models face obstacles in industrial application.To address these limitations,we developed and improved an AMP predi...Owing to their limited accuracy and narrow applicability,current antimicrobial peptide(AMP)prediction models face obstacles in industrial application.To address these limitations,we developed and improved an AMP prediction model using Comparing and Optimizing Multiple DEep Learning(COMDEL)algorithms,coupled with high-throughput AMP screening method,finally reaching an accuracy of 94.8%in test and 88%in experiment verification,surpassing other state-of-the-art models.In conjunction with COMDEL,we employed the phage-assisted evolution method to screen Sortase in vivo and developed a cell-free AMP synthesis system in vitro,ultimately increasing AMPs yields to a range of 0.5-2.1 g/L within hours.Moreover,by multi-omics analysis using COMDEL,we identified Lactobacillus plantarum as the most promising candidate for AMP generation among 35 edible probiotics.Following this,we developed a microdroplet sorting approach and successfully screened three L.plantarum mutants,each showing a twofold increase in antimicrobial ability,underscoring their substantial industrial application values.展开更多
Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） ...Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） with attractive luminescent properties may be promising candidates to overcome such challenges. Here, a novel one-step synthetic method is presented to prepare highly fluorescent copper NCs （CuNCs） in ambient conditions by using glutathione （GSH） as both the reducing agent and the protective layer preventing the aggregation of the as-formed NCs. The resultant CuNCs, with an average diameter of 2.3 nm, contain 1-3 atoms and exhibit red fluorescence （A^m = 610 nm） with high quantum yields （QYs, up to 5.0%）. Interestingly, the fluorescence signal of the CuNCs is reversibly responsive to the environmental temperature in the range of 15-80 ℃. Furthermore, as the CuNCs exhibit good biocompatibility, they can pervade the MC3T3-E1 cells and enable measurements over the physiological temperature range of 15-45 ℃ with the use of the confocal fluorescence imaging method. In view of the facile synthesis method and attractive fluorescence properties, the as-prepared CuNCs may be used as photoluminescence thermometers and biosensors.展开更多
It is of great significance to synthesize carbon dots(CDs)with desirable hydrophilicity for the ever-growing application of CDs in different fields.In this study,the hydrophilic and hydrophobic CDs were facilely prepa...It is of great significance to synthesize carbon dots(CDs)with desirable hydrophilicity for the ever-growing application of CDs in different fields.In this study,the hydrophilic and hydrophobic CDs were facilely prepared by solvothermal treatment of o-dihydroxybenzene and urea in N,N-dimethylformamide(DMF).Optimization experiments revealed that the solvothermal temperature has a great impact on the surface states of the CDs.The hydrophobic CDs with a contact angle of 110.7°was obtained at 200℃.The structural and optical characterizations,along with theoretical calculations elucidated that the lipophilic nature of the CDs was resulting from the formation of polymer chains.The presence of extended conjugated sp^(2)-domains and amino groups contributed to the red emission of the CDs synthesized at low reaction temperatures(160-200℃).With the further increase of solvothermal temperature,the hydrophobic CDs were gradually transformed to the hydrophilic state accompanying the blue shift of the fluorescence of the CDs.The highly hydrophilic CDs with a contact angle of 25.9°were obtained at 240℃ due to the increased formation of hydrophilic functional groups on the surface of CDs.The red emissive CDs exhibited a sensitive color and fluorescence response to ethanol content while the fluorescence of the blue emissive CDs remained constant.By combining the two kinds of CDs,a dual-emission sensor was constructed,which was successfully applied for the evaluation of the alcoholic strength in commercial Baijiu commodities in both fluorometric and colorimetric modes.展开更多
As a new type of fluorescent nanomaterials,carbon dots(CDs)have exhibited excellent photoluminescence properties with tunable emission and high quantum yields,hence they have attracted an increasing interest in divers...As a new type of fluorescent nanomaterials,carbon dots(CDs)have exhibited excellent photoluminescence properties with tunable emission and high quantum yields,hence they have attracted an increasing interest in diverse research areas.The photoluminescence performance of CDs is primarily influenced by their precursors,which directly or indirectly determine the structures and specific functions of the resultant CDs.In this review,we aim to summarize the recent progress on synthesis of CDs using small aliphatic molecules,anilines,polyphenol,polycyclic aromatic hydrocarbons,organic dyes,or biomass as precursors.The associations of the physical and chemical properties of the CDs with their respective precursors are comprehensively investigated,and the potential applications and future development of CDs are discussed in detail.It is hoped that this review will open new horizons for CDs preparation by rational selection of the precursors from the vastly available carbon sources and the critical comments presented,here could inspire and guide future research in the design of multifunctional CDs.展开更多
A single particle aerosol mass spectrometer(SPAMS)was used to accurately quantify the contribution of vehicle non-exhaust emissions to particulate matter at typical road environment.The PM_(2.5),black carbon,meteorolo...A single particle aerosol mass spectrometer(SPAMS)was used to accurately quantify the contribution of vehicle non-exhaust emissions to particulate matter at typical road environment.The PM_(2.5),black carbon,meteorological parameters and traffic flow were recorded during the test period.The daily trend for traffic flow and speed on TEDA Street showed obvious“M”and“W”characteristics.6.3 million particles were captured via the SPAMS,including 1.3 million particles with positive and negative spectral map information.Heavy Metal,High molecular Organic Carbon,Organic Carbon,Mixed Carbon,Elemental Carbon,Rich Potassium,Levo-rotation Glucose,Rich Na,SiO_(3) and other categories were analyzed.The particle number concentration measured by SPAMS showed a good linear correlation with the mass concentrations of PM_(2.5) and BC,which indicates that the particulate matter captured by the SPAMS reflects the pollution level of fine particulate matter.EC,ECOC,OC,HM and crustal dust components were found to show high values from 7:00–9:00 AM,showing that these chemical components are directly or indirectly related to vehicle emissions.Based on the PMF model,7 major factors are resolved.The relative contributions of each factor were determined:vehicle exhaust emission(44.8%),coal-fired source(14.5%),biomass combustion(12.2%),crustal dust(9.4%),ship emission(9.0%),tires wear(6.6%)and brake pads wear(3.5%).The results show that the contribution of vehicle non-exhaust to particulate matter at roadside environment is approximately 10.1%.Vehicle non-exhaust emissions are the focus of future research in the vehicle pollutant emission control field.展开更多
An efficient photo-Fenton catalyst(Fe S_(2)@HTCN)was designed by maximizing the synergistic effect of Fe S_(2)nanoparticles and hollow tubular g-C_(3)N_(4)(HTCN).Molecule self-assembly and molten salts-assisted calcin...An efficient photo-Fenton catalyst(Fe S_(2)@HTCN)was designed by maximizing the synergistic effect of Fe S_(2)nanoparticles and hollow tubular g-C_(3)N_(4)(HTCN).Molecule self-assembly and molten salts-assisted calcination were used to engineering the hollow structured g-C_(3)N_(4)before anchoring Fe S_(2)nanoparticles on the walls of HTCN via reflux method.Compared to bulk g-C_(3)N_(4),the unique structure of HTCN and heterojunction in the composite endowed FeS_(2)@HTCN with more active sites and abundant channels for electron transfer and charge separation.The enriched electrons can improve the Fe^(3+) recycling and boost Fe^(2+) catalyzed ^(·)OH production via H_(2)O_(2).As-prepared photo-Fenton catalyst was successfully applied to the treatment of industrial paint wastewater.The paint wastewater with its COD as high as 8200 mg/L can be effectively degraded with 0.2 mol/L H_(2)O_(2)in 90 min under visible light irradiation.The photoFenton system was further evaluated according to the process stability and economic benefit,proving that the strategy presented in this work would be applicable to the treatment of real wastewater.展开更多
基金the Natural National Science Foundation of China (No. 51973083)the Fundamental Research Funds for the Central Universities (No. JUSRP22027)clinical research and translational medicine program of affiliated hospital of Jiangnan University (No. LCYJ202239).
文摘Carbon dots(CDs)with superior fluorescence properties have attracted a growing number of research interests in anti-counterfeiting.However,the preparation of CDs with thermally turn-on fluorescence and full-color-emitting in visible spectrum is still a big challenge due to the complicated reaction mechanism in the formation of CDs.Here,a simple precursor-oriented strategy for the preparation of multicolor CDs with heat-stimuli turn-on fluorescence is reported.Comprehensive experimental characterizations and theoretical calculations revealed that the emission wavelength of CDs can be readily tuned from 460 nm to 654 nm with selected precursors,which was ascribed to the extent of conjugated sp2-domains(core states)and the amount of oxygen-and nitrogen-containing groups bound to sp2-domains(surface states).After simply mixing two or three kinds of CDs,a full-color range of fluorescence emission was realized,and the CDs-based fluorescence inks were successfully fabricated.Particularly,all the printed patterns from the inkjet exhibited a thermal-induced enhancement in fluorescence.On this basis,combining CDs with heating-induced“turn-off”fluorescence materials can lead to multidimensional and multistage encryption.These results demonstrate that the thermochromic and photochromic CDs with much more enhanced security exhibit promising application in data storage and encryption.
基金supported by a grant from the Hubei University of Science and Technology Program(No.BK202417,China)Doctoral Special Research Fund Launch Project of Jiamusi University(JMSUBZ2021-12,China)Youth Innovative Talent Cultivation Support Plan of Jiamusi University(JMSUQP2022016,China)。
文摘Owing to their limited accuracy and narrow applicability,current antimicrobial peptide(AMP)prediction models face obstacles in industrial application.To address these limitations,we developed and improved an AMP prediction model using Comparing and Optimizing Multiple DEep Learning(COMDEL)algorithms,coupled with high-throughput AMP screening method,finally reaching an accuracy of 94.8%in test and 88%in experiment verification,surpassing other state-of-the-art models.In conjunction with COMDEL,we employed the phage-assisted evolution method to screen Sortase in vivo and developed a cell-free AMP synthesis system in vitro,ultimately increasing AMPs yields to a range of 0.5-2.1 g/L within hours.Moreover,by multi-omics analysis using COMDEL,we identified Lactobacillus plantarum as the most promising candidate for AMP generation among 35 edible probiotics.Following this,we developed a microdroplet sorting approach and successfully screened three L.plantarum mutants,each showing a twofold increase in antimicrobial ability,underscoring their substantial industrial application values.
基金This work was supported by National Natural Science Foundation of China （Nos. 51372265 and 21175060）, the Natural Science Foundation of Jiangsu Province, China （No. BK20140392）, the Open Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering of Nanjing University of Technology （2014, KL14-12）, the Postdoctoral Research Foundation of Jiangsu Province, China （No. 1401058B）, and the Science and Technology Project of Suzhou, China （Nos~ ZXG201428 and ZXG201401）~
文摘Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） with attractive luminescent properties may be promising candidates to overcome such challenges. Here, a novel one-step synthetic method is presented to prepare highly fluorescent copper NCs （CuNCs） in ambient conditions by using glutathione （GSH） as both the reducing agent and the protective layer preventing the aggregation of the as-formed NCs. The resultant CuNCs, with an average diameter of 2.3 nm, contain 1-3 atoms and exhibit red fluorescence （A^m = 610 nm） with high quantum yields （QYs, up to 5.0%）. Interestingly, the fluorescence signal of the CuNCs is reversibly responsive to the environmental temperature in the range of 15-80 ℃. Furthermore, as the CuNCs exhibit good biocompatibility, they can pervade the MC3T3-E1 cells and enable measurements over the physiological temperature range of 15-45 ℃ with the use of the confocal fluorescence imaging method. In view of the facile synthesis method and attractive fluorescence properties, the as-prepared CuNCs may be used as photoluminescence thermometers and biosensors.
基金supported by the National Natural Science Foundation of China(Nos.51973083,22376081)the Fundamental Research Funds for the Central Universities(No.JUSRP22027).
文摘It is of great significance to synthesize carbon dots(CDs)with desirable hydrophilicity for the ever-growing application of CDs in different fields.In this study,the hydrophilic and hydrophobic CDs were facilely prepared by solvothermal treatment of o-dihydroxybenzene and urea in N,N-dimethylformamide(DMF).Optimization experiments revealed that the solvothermal temperature has a great impact on the surface states of the CDs.The hydrophobic CDs with a contact angle of 110.7°was obtained at 200℃.The structural and optical characterizations,along with theoretical calculations elucidated that the lipophilic nature of the CDs was resulting from the formation of polymer chains.The presence of extended conjugated sp^(2)-domains and amino groups contributed to the red emission of the CDs synthesized at low reaction temperatures(160-200℃).With the further increase of solvothermal temperature,the hydrophobic CDs were gradually transformed to the hydrophilic state accompanying the blue shift of the fluorescence of the CDs.The highly hydrophilic CDs with a contact angle of 25.9°were obtained at 240℃ due to the increased formation of hydrophilic functional groups on the surface of CDs.The red emissive CDs exhibited a sensitive color and fluorescence response to ethanol content while the fluorescence of the blue emissive CDs remained constant.By combining the two kinds of CDs,a dual-emission sensor was constructed,which was successfully applied for the evaluation of the alcoholic strength in commercial Baijiu commodities in both fluorometric and colorimetric modes.
基金supported by the National Natural Science Foundation of China(No.51973083)Fundamental Research Funds for the Central Universities(No.JUSRP22027).
文摘As a new type of fluorescent nanomaterials,carbon dots(CDs)have exhibited excellent photoluminescence properties with tunable emission and high quantum yields,hence they have attracted an increasing interest in diverse research areas.The photoluminescence performance of CDs is primarily influenced by their precursors,which directly or indirectly determine the structures and specific functions of the resultant CDs.In this review,we aim to summarize the recent progress on synthesis of CDs using small aliphatic molecules,anilines,polyphenol,polycyclic aromatic hydrocarbons,organic dyes,or biomass as precursors.The associations of the physical and chemical properties of the CDs with their respective precursors are comprehensively investigated,and the potential applications and future development of CDs are discussed in detail.It is hoped that this review will open new horizons for CDs preparation by rational selection of the precursors from the vastly available carbon sources and the critical comments presented,here could inspire and guide future research in the design of multifunctional CDs.
基金supported by the National Natural Science Foundation of China(Nos.42107114 and 42177084)the Tianjin Science and Technology Plan Project(No.20YFZCSN01000)the Fundamental Research Funds for the Central Universities(No.63221411).
文摘A single particle aerosol mass spectrometer(SPAMS)was used to accurately quantify the contribution of vehicle non-exhaust emissions to particulate matter at typical road environment.The PM_(2.5),black carbon,meteorological parameters and traffic flow were recorded during the test period.The daily trend for traffic flow and speed on TEDA Street showed obvious“M”and“W”characteristics.6.3 million particles were captured via the SPAMS,including 1.3 million particles with positive and negative spectral map information.Heavy Metal,High molecular Organic Carbon,Organic Carbon,Mixed Carbon,Elemental Carbon,Rich Potassium,Levo-rotation Glucose,Rich Na,SiO_(3) and other categories were analyzed.The particle number concentration measured by SPAMS showed a good linear correlation with the mass concentrations of PM_(2.5) and BC,which indicates that the particulate matter captured by the SPAMS reflects the pollution level of fine particulate matter.EC,ECOC,OC,HM and crustal dust components were found to show high values from 7:00–9:00 AM,showing that these chemical components are directly or indirectly related to vehicle emissions.Based on the PMF model,7 major factors are resolved.The relative contributions of each factor were determined:vehicle exhaust emission(44.8%),coal-fired source(14.5%),biomass combustion(12.2%),crustal dust(9.4%),ship emission(9.0%),tires wear(6.6%)and brake pads wear(3.5%).The results show that the contribution of vehicle non-exhaust to particulate matter at roadside environment is approximately 10.1%.Vehicle non-exhaust emissions are the focus of future research in the vehicle pollutant emission control field.
基金the Natural National Science Foundation of China(No.51973083)National First-Class Discipline Program of Food Science and Technology(No.JUFSTR20180301)+1 种基金China Postdoctoral Science Foundation(No.2019M651688)Fundamental Research Funds for the Central Universities(No.JUSRP22027)。
文摘An efficient photo-Fenton catalyst(Fe S_(2)@HTCN)was designed by maximizing the synergistic effect of Fe S_(2)nanoparticles and hollow tubular g-C_(3)N_(4)(HTCN).Molecule self-assembly and molten salts-assisted calcination were used to engineering the hollow structured g-C_(3)N_(4)before anchoring Fe S_(2)nanoparticles on the walls of HTCN via reflux method.Compared to bulk g-C_(3)N_(4),the unique structure of HTCN and heterojunction in the composite endowed FeS_(2)@HTCN with more active sites and abundant channels for electron transfer and charge separation.The enriched electrons can improve the Fe^(3+) recycling and boost Fe^(2+) catalyzed ^(·)OH production via H_(2)O_(2).As-prepared photo-Fenton catalyst was successfully applied to the treatment of industrial paint wastewater.The paint wastewater with its COD as high as 8200 mg/L can be effectively degraded with 0.2 mol/L H_(2)O_(2)in 90 min under visible light irradiation.The photoFenton system was further evaluated according to the process stability and economic benefit,proving that the strategy presented in this work would be applicable to the treatment of real wastewater.
