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In vivo theranostics with near-infraredemitting carbon dots—highly efficient photothermal therapy based on passive targeting after intravenous administration 被引量:21
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作者 Xin Bao Ye Yuan +13 位作者 Jingqin Chen Bohan Zhang Di Li Ding Zhou pengtao Jing Guiying Xu Yingli Wang Kateřina Holá Dezhen Shen Changfeng Wu Liang song Chengbo Liu Radek Zbořil songnan Qu 《Light(Science & Applications)》 SCIE EI CAS CSCD 2018年第1期182-192,共11页
Carbon dots that exhibit near-infrared fluorescence(NIR CDs)are considered emerging nanomaterials for advanced biomedical applications with low toxicity and superior photostability and targeting compared to currently ... Carbon dots that exhibit near-infrared fluorescence(NIR CDs)are considered emerging nanomaterials for advanced biomedical applications with low toxicity and superior photostability and targeting compared to currently used photoluminescence agents.Despite progress in the synthesis of NIR CDs,there remains a key obstacle to using them as an in vivo theranostic agent.This work demonstrates that the newly developed sulfur and nitrogen codoped NIR CDs are highly efficient in photothermal therapy(PTT)in mouse models(conversion efficiency of 59%)and can be readily visualized by photoluminescence and photoacoustic imaging.The real theranostic potential of NIR CDs is enhanced by their unique biodistribution and targeting.Contrary to all other nanomaterials that have been tested in biomedicine,they are excreted through the body’s renal filtration system.Moreover,after intravenous injection,NIR CDs are accumulated in tumor tissue via passive targeting,without any active species such as antibodies.Due to their accumulation in tumor tissue without the need for intratumor injection,high photothermal conversion,excellent optical and photoacoustic imaging performance,and renal excretion,the developed CDs are suitable for transfer to clinical biomedical practice. 展开更多
关键词 INTRAVENOUS injection VIVO
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Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses 被引量:11
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作者 song Yuwei KANG Yanli LIU Hao ZHAO Xiaoliang WANG pengtao AN Guoyong ZHOU Yun MIAO Chen song Chunpeng 《Chinese Science Bulletin》 SCIE EI CAS 2006年第21期2586-2594,共9页
In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appro- priate state. However, ma... In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appro- priate state. However, many signaling components in guard cell signaling remain elusive. In our laboratory, a tool for non-invasive remote infrared thermal images was used to screen an ethyl methane sulfonate-mutagenized population for Arabidopsis stomatal response mutants under multiple stresses (ABA, H2O2, CO2, etc.). More than forty "hot" or "cold" mutants were isolated (above or below 0.5℃ in con- trast to normal plantlets). Identification and primary genetic analysis of these mutants show that they are monogenic recessive mutations and there exist distinct difference in stomata apertures compared to wild type. These mutants in response to various environmental stresses and hormones were comprehen- sively investigated, which enables us to further un- derstand the cross-talk in different signal transduction pathways. 展开更多
关键词 红外摄象机 保卫细胞 遗传分析 信号转导变异
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MAP kinase specifically mediates the ABA-induced H_2O_2 generation in guard cells of Vicia faba L. 被引量:10
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作者 JIANG Jing1,2, AN Guoyong1, WANG Pengcheng1, WANG pengtao1, HAN Jinfeng2, JIA Yanbin1 & song Chunpeng1 1. College of Life Sciences, Henan University, Kaifeng 475001, China 2. College of Agriculture Science, Henan Agriculture University, Zheng-zhou 450001, China Correspondence should be addressed to song Chunpeng (e-mail: songcp @henu. edu.cn) 《Chinese Science Bulletin》 SCIE EI CAS 2003年第18期1919-1926,共8页
Mitogen-activated protein (MAP) kinase is involved in ABA- or H2O2-signaling, and H2O2 acts as inter-mediate in the downstream of ABA signal transduction pathway, which has recently emerged as a secondary mes-senger o... Mitogen-activated protein (MAP) kinase is involved in ABA- or H2O2-signaling, and H2O2 acts as inter-mediate in the downstream of ABA signal transduction pathway, which has recently emerged as a secondary mes-senger of ABA signaling. Using an epidermal strip bioassay and laser scanning confocal microscope, we provided the first evidence that MAP kinase plays an important role in H2O2 signal initial, amplification and specific targeting in response to stimuli in guard cells. ABA- or H2O2-induced Vicia faba stomatal closure was inhibited or reversed by the specific inhibitor PD98059 of MEK1/2; the guard cells were pre-incubated or -microinjected by 10 mmol·L-1 PD98059, ABA could not enhance the fluorescence intensity of H2O2 probe dichlorofluorescein (DCF). Meanwhile, after ABA induced the H2O2 accumulation in guard cells, the exogenous or intracellular PD98059 could reduce the DCF fluorescence intensity. Most interestingly, on the contrary to ABA, the DCF fluorescence intensity of guard cells treated by 100 mmol·L-1 salicylic acid (SA) was not down-regulated by PD98059, yet PD98059 did not regulate the stomatal move-ment being induced by light, dark or salicylic acid. These results suggest that MEK1/2 could mediate stomatal closure by abolishing the ABA-induced H2O2 generation/accumula- tion in the specific manner. 展开更多
关键词 MAP KINASE PD98059 ABA H2O2 GUARD cells.
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基于“肺与大肠相表里”理论探讨肠屏障损伤与慢性阻塞性肺疾病的关系 被引量:2
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作者 梁鹏涛 仇振明 +2 位作者 宋丽云 孙仪 羊忠山 《实用中医内科杂志》 2024年第1期16-20,共5页
在中西医不同的理论里,肺与大肠均存在密切的联系,在西医的理论里,肺与大肠在分化发育、生理病理、功能协作、物质联系上有结构、功能、菌群基础作为支撑依据,而这种联系与中医经典《黄帝内经》中所讲“肺与大肠相表里”不谋而合,在中... 在中西医不同的理论里,肺与大肠均存在密切的联系,在西医的理论里,肺与大肠在分化发育、生理病理、功能协作、物质联系上有结构、功能、菌群基础作为支撑依据,而这种联系与中医经典《黄帝内经》中所讲“肺与大肠相表里”不谋而合,在中医的“肺与大肠相表里”理论里肺与大肠通过经脉相络属,生理相协调以达到相互联系的目的,在汗牛充栋的历代医案中,也不乏肺病从肠论治,肠病从肺论治的治疗策略,现代医学研究发现,肠屏障的损伤与慢性阻塞性肺疾病之间可相互影响彼此的疾病病理发展进程,其中微生物屏障(主要为肠道菌群)可能是将肺与大肠进行动态联系的重要活性基础,其调节方式主要通过肠道菌群代谢产物实现,如维生素、短链脂肪酸等,因此维持肠道菌群的动态平衡及修复受损的肠屏障可能成为慢性阻塞性肺疾病的一种潜在治疗策略。文章通过系统归纳总结梳理了目前国内外学者对于肺与大肠在肠屏障损伤与慢性阻塞性肺疾病之间的潜在联系及最新的研究进展,综述了有关于肺与大肠在分化发育、生理病理、功能协作及物质联系之间的相互关系,阐释肠屏障损伤与慢性阻塞性肺疾病之间相互影响的联系,以期对该理论有进一步的认识并为临床上对肺肠疾病的治疗提供新思路。 展开更多
关键词 肺与大肠相表里 肠屏障 慢性阻塞性肺疾病 肠道菌群 紧密连接
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Reactive oxygen species:Multidimensional regulators of plant adaptation to abiotic stress and development 被引量:1
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作者 pengtao Wang Wen-Cheng Liu +3 位作者 Chao Han Situ Wang Ming-Yi Bai Chun-Peng song 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第3期330-367,共38页
Reactive oxygen species(ROS)are produced as undesirable by-products of metabolism in various cellular compartments,especially in response to unfavorable environmental conditions,throughout the life cycle of plants.Str... Reactive oxygen species(ROS)are produced as undesirable by-products of metabolism in various cellular compartments,especially in response to unfavorable environmental conditions,throughout the life cycle of plants.Stressinduced ROS production disrupts normal cellular function and leads to oxidative damage.To cope with excessive ROS,plants are equipped with a sophisticated antioxidative defense system consisting of enzymatic and non-enzymatic components that scavenge ROS or inhibit their harmful effects on biomolecules.Nonetheless,when maintained at relatively low levels,ROS act as signaling molecules that regulate plant growth,development,and adaptation to adverse conditions.Here,we provide an overview of current approaches for detecting ROS.We also discuss recent advances in understanding ROS signaling,ROS metabolism,and the roles of ROS in plant growth and responses to various abiotic stresses. 展开更多
关键词 abiotic stress growth and development PLANT reactive oxygen species
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A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis 被引量:6
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作者 WANG pengtao LIU Hao HUA HongJie WANG Lei song Chun-Peng 《Chinese Science Bulletin》 SCIE EI CAS 2011年第33期3538-3546,共9页
The phytohormone abscisic acid (ABA) plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological ... The phytohormone abscisic acid (ABA) plays a critical role in plant growth, development, and adaptation to various stress conditions. The cellular ABA level is constantly adjusted to respond to changing physiological and environmental conditions. To date, the mechanisms for fine-tuning ABA levels remain elusive. Here, we report that BGLU10, a member of a multigene family of β-glucosidases, contributes to drought tolerance in Arabidopsis. The T-DNA insertion mutant bglu10 exhibited a droughtsensitive phenotype, characterized by an increased rate of water loss, and lower leaf temperature, β-glucosidase activity, ABA content, and expressions of ABA-and drought-responsive genes under drought stress. In contrast, lines overexpressing BGLU10 showed greater drought resistance than that of the wild-type, as shown by decreased water loss via transpiration, higher β-glucosidase activity, ABA level, and expressions of ABA- and stress-responsive genes under drought stress. Transient expression of BGLU10::GFP and γ-TIP1::RFP in mesophyll cell protoplasts showed that the BGLU10 enzyme protein was localized to the vacuole. Meanwhile, BGLU10 was expressed in various organs, and was induced by several abiotic stresses, suggesting that BGLU10 may be involved in a variety of stress responses, and that hydrolysis of ABA-GE produces free ABA in the plant stress response. 展开更多
关键词 葡萄糖苷酶 抗旱性 拟南芥 液泡 ABA含量 本地化 多基因家族 干旱胁迫
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胶东大尹格庄金矿成矿流体时空演化及矿床成因:来自流体包裹体、成矿元素和H-O-S-Pb同位素证据
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作者 严子清 石文杰 +6 位作者 张鹏涛 王勇军 邵玉宝 黄鑫 宋宇 谭俊 王治华 《地质科技通报》 CAS CSCD 北大核心 2024年第2期156-174,共19页
大尹格庄金矿位于招平成矿带中段,是胶东地区典型的构造蚀变岩型金矿床,储量达到超大型规模,但关于该矿床的成因类型尚存在较大争议。在详细野外地质调查的基础上开展了该矿床成因和成矿流体纵向变化特征研究。流体包裹体研究表明,成矿... 大尹格庄金矿位于招平成矿带中段,是胶东地区典型的构造蚀变岩型金矿床,储量达到超大型规模,但关于该矿床的成因类型尚存在较大争议。在详细野外地质调查的基础上开展了该矿床成因和成矿流体纵向变化特征研究。流体包裹体研究表明,成矿流体为中温、低盐度、中低密度的H_(2)O-CO_(2)-NaCl±CH_(4)体系。从成矿早期到晚期各阶段(Ⅰ~Ⅳ阶段)均一温度和盐度逐渐降低,密度逐渐增加。氢氧同位素组成显示成矿流体早期以岩浆水为主,后期有大气降水的混入,主成矿阶段可能存在流体沸腾作用;黄铁矿硫铅同位素组成表明成矿物质来源于深源壳幔混合岩浆。成矿过程和背景总体与胶东其他金矿床类似,形成于克拉通破坏环境。浅部与深部流体和物质组成的对比研究表明,在垂向纵深范围内成矿流体性质、金银成矿强度和金成色稳定一致,金沉淀具有宽泛而稳定的环境,指示大尹格庄金矿床深部仍然具有很大的成矿潜力和找矿空间。 展开更多
关键词 流体包裹体 H-O-S-Pb同位素 金成色 矿床成因 深部找矿 成矿流体 大尹格庄
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2-羟甲基环戊酮的合成及核磁研究
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作者 张桂红 闻硕 +2 位作者 白鹏涛 宋恒 徐晨 《化学研究》 CAS 2024年第2期123-126,共4页
以环戊酮-2-羧酸甲酯为原料,通过缩醛、还原、羰基脱保护三步反应合成2-羟甲基环戊酮。对第三步反应经行优化,在水相中廉价β环糊精高效促进羰基脱保护反应,操作简单、环境友好、经济高效,产率99%。中间产物和目标产物经NMR表征,并详细... 以环戊酮-2-羧酸甲酯为原料,通过缩醛、还原、羰基脱保护三步反应合成2-羟甲基环戊酮。对第三步反应经行优化,在水相中廉价β环糊精高效促进羰基脱保护反应,操作简单、环境友好、经济高效,产率99%。中间产物和目标产物经NMR表征,并详细研究目标产物及其活泼氢的NMR。不同氘代试剂中,产物活泼氢出现在不同位置,通过重水交换,确定产物活泼氢的位置。 展开更多
关键词 2-羟甲基环戊酮 核磁 活泼氢
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凹唇壁蜂雌成虫滞育期、休眠期和破茧期肠道菌群多样性分析
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作者 王光召 马艺翔 +9 位作者 秦敬泽 游银伟 马朋涛 李丽莉 门兴元 宋莹莹 张凯 李霞 王桂萍 于毅 《植物保护学报》 CAS CSCD 北大核心 2024年第3期556-567,共12页
为明确凹唇壁蜂Osmia excavata雌成虫滞育期至破茧期肠道菌群的多样性及差异,解析其在宿主健康与调控宿主生长发育中的作用,采用Illumina NovaSeq二代高通量测序技术对不同时期肠道细菌16S rRNA V3~V4区域进行测序,分析雌成虫滞育期至... 为明确凹唇壁蜂Osmia excavata雌成虫滞育期至破茧期肠道菌群的多样性及差异,解析其在宿主健康与调控宿主生长发育中的作用,采用Illumina NovaSeq二代高通量测序技术对不同时期肠道细菌16S rRNA V3~V4区域进行测序,分析雌成虫滞育期至破茧期肠道菌群的多样性变化。结果显示,凹唇壁蜂雌成虫在滞育期至破茧期肠道细菌菌群中共检测到41门96纲198目325科637属,其中核心菌门是变形菌门,相对丰度介于75.98%~91.41%之间;核心菌属是Sodalis,相对丰度介于59.65%~74.66%之间。Alpha多样性分析显示,从滞育期到休眠期,雌成虫肠道内菌群多样性指数明显升高,从休眠期至破茧期,其肠道菌群多样性指数出现降低趋势;休眠期其肠道菌群的物种丰富度和均匀度均最高,滞育期其肠道菌群的物种丰富度和均匀度均最低;Beta多样性分析结果显示,从滞育期至破茧期,其肠道菌群结构相对稳定;LEfSe分析结果显示,从滞育期至破茧期其肠道菌群在门、纲、目、科、属水平均存在显著差异,且差异显著菌群主要出现在休眠期。预测的不同时期的肠道菌群功能不同,这与其成虫解除滞育进入休眠再破茧的生理过程密切相关。表明凹唇壁蜂雌成虫从滞育期至破茧期肠道核心细菌种类一致,群落结构相对稳定,但是群落多样性发生明显变化。 展开更多
关键词 凹唇壁蜂 滞育 休眠 破茧 肠道菌群 多样性
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GA Associated Dwarf 5 encodes an ent-kaurenoic acid oxidase required for maize gibberellin biosynthesis and morphogenesis 被引量:1
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作者 Zuliang Li Baozhu Li +8 位作者 Junli Zhang Hongliang Wang Mao Wang Siyi Guo pengtao Wang Zhi Li David WGalbraith Dandan Li Chun-Peng song 《The Crop Journal》 SCIE CSCD 2023年第6期1742-1751,共10页
Gibberellin(GA)functions in plant growth and development.However,genes involved in the biosynthesis and regulation of GA in crop plants are poorly understood.We isolated the mutant gad5-1(GAAssociated Dwarf 5),charact... Gibberellin(GA)functions in plant growth and development.However,genes involved in the biosynthesis and regulation of GA in crop plants are poorly understood.We isolated the mutant gad5-1(GAAssociated Dwarf 5),characterized by dwarfing,short internodes,and dark green and short leaves.Map-based gene cloning and allelic verification confirmed that ZmGAD5 encodes ent-kaurenoic acid oxidase(KAO),which catalyzes KA(ent-kaurenoic acid)to GA12 conversion during GA biosynthesis in maize.ZmGAD5 is localized to the endoplasmic reticulum and is present in multiple maize organs.In gad5-1,the expression of ZmGAD5 is severely reduced,and the levels of the direct substrate of KAO,KA,is increased,leading to a reduction in GA content.The abnormal phenotype of gad5-1 was restored by exogenous application of GA3.The biomass,plant height,and levels of GA12 and GA3 in transgenic Arabidopsis overexpressing ZmGAD5 were increased in comparison with the corresponding controls Col-0.These findings deepen our understanding of genes involved in GA biosynthesis,and could lead to the development of maize lines with improved architecture and higher planting-density tolerance. 展开更多
关键词 Maize(Zea mays L.) ZmGAD5 GA biosynthesis KAO
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时间测序下基于SVM的用户异常检测研究
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作者 彭英 杨澎涛 +1 位作者 宋建 孙友凯 《信息技术与信息化》 2023年第1期130-133,138,共5页
随着我国信息科技的飞跃式发展,计算机网络的规模不断扩大,企业扩展业务持续增加,油田内部信息的机密性与设备的安全性面临着巨大挑战。针对目前油田网络系统繁杂性不断增大而引发的安全的问题,运用基于简单网络管理协议设计实现的网内... 随着我国信息科技的飞跃式发展,计算机网络的规模不断扩大,企业扩展业务持续增加,油田内部信息的机密性与设备的安全性面临着巨大挑战。针对目前油田网络系统繁杂性不断增大而引发的安全的问题,运用基于简单网络管理协议设计实现的网内流量监控,获取工业网流量信息,在时间测序下,借助机器学习与文本分析整合异常数据的共性与特性,关联整合异常数据的多维关系,解除了以往繁杂且低效的监控手段,保证网络环境内异常信息的可见性,极大便利了后期的维护工作和网络环境的平滑升级。 展开更多
关键词 SNMP 文本分析 机器学习 异常检测
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Continuous-Variable Quantum Computation in Circuit QED
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作者 Xiaozhou Pan pengtao song Yvonne Y.Gao 《Chinese Physics Letters》 SCIE EI CAS CSCD 2023年第11期14-17,共4页
Quantum computation (QC) offers the promise of enhanced computational capabilities and drastic accelerations in solving complex tasks such as quantum chemistry^([1,2])and prime factorization.^([3])Various physical imp... Quantum computation (QC) offers the promise of enhanced computational capabilities and drastic accelerations in solving complex tasks such as quantum chemistry^([1,2])and prime factorization.^([3])Various physical implementations for practical QC are being pursued across the academic and industrial research initiatives.Exemplary ones include trapped ions,^([4])nuclear spin,^([5]). 展开更多
关键词 ACCELERATION QUANTUM QUANTUM
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人类精原细胞分化过程的生物信息学分析
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作者 殷晨星 李澎涛 +6 位作者 宋静岚 师楠 陈英 邢鹏 孟娜娜 张康 王玉真 《中华生殖与避孕杂志》 CAS CSCD 北大核心 2023年第12期1237-1243,共7页
目的为了明确精子发生过程中的关键基因和生物学过程, 挖掘人类精原细胞(spermatogonia, SPG)分化过程中的差异表达基因(differentially expressed genes, DEGs)及其信号通路, 探索精子发生早期的分子机制, 增加对SPG分化过程的分子生... 目的为了明确精子发生过程中的关键基因和生物学过程, 挖掘人类精原细胞(spermatogonia, SPG)分化过程中的差异表达基因(differentially expressed genes, DEGs)及其信号通路, 探索精子发生早期的分子机制, 增加对SPG分化过程的分子生物学认识。方法从公共数据库下载人类未分化精原细胞(undifferentiated spermatogonia, uSPG)和分化精原细胞(differentiating spermatogonia, dSPG)的转录组数据, 利用Hisat2和StingTie筛选DEGs, 并对差异表达基因进行GO和KEGG功能富集分析。利用MACS2软件对ATAC-seq数据中开放染色质区域进行富集分析。结果共筛选出8 532个DEGs, 包括4 127个上调基因和4 405个下调基因, 它们通过一些重要的生物学过程, 如细胞周期、细胞因子介导的信号通路、有机物代谢过程、细胞运动、甲基化等来调控SPG的分化过程。KEGG富集分析结果表明一些重要信号通路包括FoxO信号通路、JAK-STAT信号通路等在SPG分化过程中具有重要作用。GO富集分析结果显示甲基化在SPG分化过程中发挥重要作用, 甲基化相关基因表达差异显著。组蛋白甲基化词条相关基因TDRDs家族显著富集, 9个TDRDs基因在dSPG中表达更加活跃。结论本研究通过生物信息学分析鉴定了SPG分化过程中的差异表达基因, 明确了关键基因在转录和染色质水平的差异, 为SPG分化机制研究奠定了重要的理论基础。 展开更多
关键词 精子发生 精原细胞 生物信息学 不育 男性 分化
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Distributed Lagrange Multiplier/Fictitious Domain Finite Element Method for a Transient Stokes Interface Problem with Jump Coefficients 被引量:2
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作者 Andrew Lundberg pengtao Sun +1 位作者 Cheng Wang Chen-song Zhang 《Computer Modeling in Engineering & Sciences》 SCIE EI 2019年第4期35-62,共28页
The distributed Lagrange multiplier/fictitious domain(DLM/FD)-mixed finite element method is developed and analyzed in this paper for a transient Stokes interface problem with jump coefficients.The semi-and fully disc... The distributed Lagrange multiplier/fictitious domain(DLM/FD)-mixed finite element method is developed and analyzed in this paper for a transient Stokes interface problem with jump coefficients.The semi-and fully discrete DLM/FD-mixed finite element scheme are developed for the first time for this problem with a moving interface,where the arbitrary Lagrangian-Eulerian(ALE)technique is employed to deal with the moving and immersed subdomain.Stability and optimal convergence properties are obtained for both schemes.Numerical experiments are carried out for different scenarios of jump coefficients,and all theoretical results are validated. 展开更多
关键词 TRANSIENT STOKES interface problem JUMP COEFFICIENTS DISTRIBUTED LAGRANGE multiplier fictitious domain method mixed finite element an optimal error estimate stability
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Observation of the exceptional point in superconducting qubit with dissipation controlled by parametric modulation 被引量:1
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作者 Zhan Wang Zhongcheng Xiang +7 位作者 Tong Liu Xiaohui song pengtao song Xueyi Guo Luhong Su He Zhang Yanjing Du Dongning Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第10期66-72,共7页
Open physical systems described by the non-Hermitian Hamiltonian with parity-time-reversal(PT)symmetry show peculiar phenomena,such as the presence of an exceptional point(EP)at which the PT symmetry is broken and two... Open physical systems described by the non-Hermitian Hamiltonian with parity-time-reversal(PT)symmetry show peculiar phenomena,such as the presence of an exceptional point(EP)at which the PT symmetry is broken and two resonant modes of the Hamiltonian become degenerate.Near the EP,the system could be more sensitive to external perturbations and this may lead to enhanced sensing.In this paper,we present experimental results on the observation of PT symmetry broken transition and the EP using a tunable superconducting qubit.The quantum system of investigation is formed by the two levels of the qubit and the energy loss of the system to the environment is controlled by a method of parametric modulation of the qubit frequency.This method is simple with no requirements for additional elements or qubit device modifications.We believe it can be easily implemented on multi-qubit devices that would be suitable for further exploration of non-Hermitian physics in more complex and diverse systems. 展开更多
关键词 exceptional point parity-time-reversal(PT)symmetry longitudinal field modulation
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Cavity-induced ATS effect on a superconducting Xmon qubit 被引量:1
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作者 Xueyi Guo Hui Deng +10 位作者 Jianghao Ding Hekang Li pengtao song Zhan Wang Luhong Su Yanjun Liu Zhongcheng Xiang Jie Li Yirong Jin Yuxi Liu Dongning Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第8期248-253,共6页
We couple a ladder-type three-level superconducting artificial atom to a cavity. Adjusting the artificial atom to make the cavity be resonant with the two upper levels, we then probe the lower two levels of the artifi... We couple a ladder-type three-level superconducting artificial atom to a cavity. Adjusting the artificial atom to make the cavity be resonant with the two upper levels, we then probe the lower two levels of the artificial atom. When driving the cavity to a coherent state, the probe spectrum shows energy level splitting induced by the quantized electromagnetic field in the cavity. This splitting size is related to the coupling strength between the cavity and the artificial atom and, thus, is fixed after the sample is fabricated. This is in contrast to the classical Autler-Townes splitting of a three-level system in which the splitting is proportional to the driving amplitude, which can be continuously changed. Our experiment results show the difference between the classical microwave driving field and the quantum field of the cavity. 展开更多
关键词 superconducting qubit circuit QED Autler-Townes splitting
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甘草酸二铵对严重烫伤大鼠肝损伤的影响及其机制
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作者 谢超琼 樊拂晓 +5 位作者 李朋涛 蔡晨 李兴照 宋均辉 徐结苟 徐庆连 《中华烧伤与创面修复杂志》 CAS CSCD 北大核心 2022年第8期735-743,共9页
目的探讨甘草酸二铵对严重烫伤大鼠肝损伤的影响及其机制。方法采用实验研究方法。取54只7~9周龄雌性SD大鼠,按随机数字表法分为背部模拟致伤的假伤组及背部造成30%体表总面积Ⅲ度烫伤的单纯烫伤组及烫伤+甘草酸二铵组,每组18只。假伤... 目的探讨甘草酸二铵对严重烫伤大鼠肝损伤的影响及其机制。方法采用实验研究方法。取54只7~9周龄雌性SD大鼠,按随机数字表法分为背部模拟致伤的假伤组及背部造成30%体表总面积Ⅲ度烫伤的单纯烫伤组及烫伤+甘草酸二铵组,每组18只。假伤组伤后不行特殊处理;单纯烫伤组及烫伤+甘草酸二铵组大鼠进行补液抗休克,烫伤+甘草酸二铵组大鼠分别于伤后1、25、49 h经腹腔注射50 mg/kg甘草酸二铵溶液。取3组大鼠,伤后24、48、72 h,采用全自动生化检测分析仪检测血清肝功能损伤相关指标天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)、总蛋白、白蛋白含量,采用酶联免疫吸附测定法检测血清鸟氨酸氨甲酰基转移酶(OCT)含量;行苏木精-伊红染色观察伤后72 h肝组织病理学变化;采用实时荧光定量反转录PCR法检测伤后24、48、72 h肝组织B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、葡萄糖调节蛋白78(GRP78)、转录激活因子4(ATF4)、蛋白激酶R样内质网激酶(PERK)的mRNA表达。采用蛋白质印迹法检测假伤组伤后72 h及烫伤2组伤后24、48、72 h肝组织Bcl-2、Bax、GRP78、PERK、ATF4的蛋白表达。各组各时间点样本数均为6。对数据行析因设计方差分析、单因素方差分析及Bonferroni检验。结果与假伤组比较,单纯烫伤组大鼠伤后各时间点血清中AST、ALT、LDH含量均明显升高(P<0.01),总蛋白、白蛋白含量均明显降低(P<0.05或P<0.01)。相较于单纯烫伤组,烫伤+甘草酸二铵组大鼠伤后24 h血清中AST含量明显下降(P<0.05);烫伤+甘草酸二铵组大鼠伤后48 h血清中AST、ALT、LDH含量均明显下降(P<0.01),总蛋白含量明显升高(P<0.01);烫伤+甘草酸二铵组大鼠伤后72 h血清中AST、ALT、LDH含量均明显下降(P<0.01),总蛋白和白蛋白含量均明显升高(P<0.01)。伤后24、48、72 h,单纯烫伤组大鼠血清OCT含量分别为(48.5±3.9)、(40.8±2.4)� 展开更多
关键词 烧伤 内质网应激 肝损伤 线粒体损伤 甘草酸二铵
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Fabrication of Josephson parameter amplifier and its application in squeezing vacuum fluctuations
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作者 pengtao song Xueyi Guo +7 位作者 Kai Xu Xiaohui song Zhan Wang Zhongcheng Xiang Hekang Li Luhong Su Yirong Jin Dongning Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第12期640-647,共8页
Josephson parameter amplifier(JPA)is a microwave signal amplifier device with near-quantum-limit-noise performance.It has important applications in scientific research fields such as quantum computing and dark matter ... Josephson parameter amplifier(JPA)is a microwave signal amplifier device with near-quantum-limit-noise performance.It has important applications in scientific research fields such as quantum computing and dark matter detection.This work reports the fabrication and characterization of broadband JPA devices and their applications in multi-qubit readout and squeezing of vacuum state.We use a process in which transmission lines and electrodes are made of niobium thin film and aluminum Josephson junctions are made by Dolan bridge technique.We believe this process is more convenient than the process we used previously.The whole production process adopts electron beam lithography technology to ensure high structural resolution.The test result shows that the gain value of the manufactured JPA can exceed 15 dB,and the amplification bandwidth is about 400 MHz.The noise temperature is about 400 mK at the working frequency of 6.2 GHz.The devices have been successfully used in experiments involving superconducting multi-qubit quantum processors.Furthermore,the device is applied to squeeze vacuum fluctuations and a squeezing level of 1.635 dB is achieved. 展开更多
关键词 Josephson parameter amplifier squeezed state quantum computing
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禽白血病病毒p27基因在毕赤酵母中的表达
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作者 曹利利 宫鹏涛 +6 位作者 姜旭 郭衍冰 宋建臣 董航 姚新华 苑淑贤 王治 《中国动物传染病学报》 CAS 北大核心 2021年第4期107-112,共6页
为获得禽白血病病毒p27蛋白,利用DNA重组技术,将扩增的p27基因亚克隆至酵母表达载体pPIC9K中,并将制备的酵母表达质粒电转至毕赤酵母细胞GS115中,通过SDS-PAGE和Western blot技术检测p27蛋白在不同条件下的表达情况,同时对诱导表达条件... 为获得禽白血病病毒p27蛋白,利用DNA重组技术,将扩增的p27基因亚克隆至酵母表达载体pPIC9K中,并将制备的酵母表达质粒电转至毕赤酵母细胞GS115中,通过SDS-PAGE和Western blot技术检测p27蛋白在不同条件下的表达情况,同时对诱导表达条件进行系统的优化。结果表明,成功构建出pPIC9K-p27酵母表达质粒,当诱导时长为24 h,甲醇诱导浓度为1%,BMMY培养基的pH值为7.0,诱导温度为28℃时,目标蛋白rp27的表达量最高可达26.2μg/mL。本研究为进一步开发禽白血病病毒检测试剂盒奠定了基础。 展开更多
关键词 禽白血病病毒 P27蛋白 表达 优化
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Investigation of dimensionality in superconducting NbN thin film samples with different thicknesses and NbTiN meander nanowire samples by measuring the upper critical field
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作者 Mudassar Nazir Xiaoyan Yang +7 位作者 Huanfang Tian pengtao song Zhan Wang Zhongcheng Xiang Xueyi Guo Yirong Jin Lixing You Dongning Zheng 《Chinese Physics B》 SCIE EI CAS CSCD 2020年第8期457-464,共8页
We study superconducting properties of NbN thin film samples with different thicknesses and an ultra-thin NbTiN meander nanowire sample.For the ultra-thin samples,we found that the temperature dependence of upper crit... We study superconducting properties of NbN thin film samples with different thicknesses and an ultra-thin NbTiN meander nanowire sample.For the ultra-thin samples,we found that the temperature dependence of upper critical field(Hc2)in parallel to surface orientation shows bending curvature close to critical temperature Tc,suggesting a two-dimensional(2D)nature of the samples.The 2D behavior is further supported by the angular dependence measurements of Hc2 for the thinnest samples.The temperature dependence of parallel upper critical field for the thick films could be described by a model based on the anisotropic Ginzburg-Landau theory.Interestingly,the results measured in the field perpendicular to the film surface orientation show a similar bending curvature but in a much narrow temperature region close to Tc for the ultra-thin samples.We suggest that this feature could be due to suppression of pair-breaking caused by local in-homogeneity.We further propose the temperature dependence of perpendicular Hc2 as a measure of uniformity of superconducting ultra-thin films.For the thick samples,we find that Hc2 shows maxima for both parallel and perpendicular orientations.The Hc2 peak for the perpendicular orientation is believed to be due to the columnar structure formed during the growth of the thick films.The presence of columnar structure is confirmed by transmission electron microscopy(TEM).In addition,we have measured the angular dependence of magneto-resistance,and the results are consistent with the Hc2 data. 展开更多
关键词 NbN micro-bridges and NbTiN meander nanowire upper critical field low dimensionality anisotropic magneto-resistance
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