对锦屏深地核天体物理实验JUNA(Jinping Underground laboratory for Nuclear Astro-physics)离子源控制系统进行了研究,采用分布式系统模型构建。硬件采用PLC、串口服务器、伺服电机及工控机等部件实现了离子源设备的远程监测及控制。...对锦屏深地核天体物理实验JUNA(Jinping Underground laboratory for Nuclear Astro-physics)离子源控制系统进行了研究,采用分布式系统模型构建。硬件采用PLC、串口服务器、伺服电机及工控机等部件实现了离子源设备的远程监测及控制。软件通过建立EPICS IOC运行时数据库,实现了对所有被控设备的集成。用户操作界面采用CSS(Control System Studio)开发,实现了操作人员对所有被控设备的透明访问。基于安全连锁规则设计了机器保护系统,实现了运行异常下的连锁保护。该控制系统应用于国内首套深地实验强流ECR离子源,运行稳定可靠,完全满足JUNA运行及物理实验的需求。展开更多
Various super-resolution microscopy techniques have been presented to explore fine structures of biological specimens.However,the super-resolution capability is often achieved at the expense of reducing imaging speed ...Various super-resolution microscopy techniques have been presented to explore fine structures of biological specimens.However,the super-resolution capability is often achieved at the expense of reducing imaging speed by either point scanning or multiframe computation.The contradiction between spatial resolution and imaging speed seriously hampers the observation of high-speed dynamics of fine structures.To overcome this contradiction,here we propose and demonstrate a temporal compressive super-resolution microscopy(TCSRM)technique.This technique is to merge an enhanced temporal compressive microscopy and a deep-learning-based super-resolution image reconstruction,where the enhanced temporal compressive microscopy is utilized to improve the imaging speed,and the deep-learning-based super-resolution image reconstruction is used to realize the resolution enhancement.The high-speed super-resolution imaging ability of TCSRM with a frame rate of 1200 frames per second(fps)and spatial resolution of 100 nm is experimentally demonstrated by capturing the flowing fluorescent beads in microfluidic chip.Given the outstanding imaging performance with high-speed super-resolution,TCSRM provides a desired tool for the studies of high-speed dynamical behaviors in fine structures,especially in the biomedical field.展开更多
目的 探讨初诊弥漫大B细胞淋巴瘤(DLBCL)并发滤泡淋巴瘤(FL)(DLBCL/FL)患者的临床病理特征、基因突变谱和预后影响因素。 方法 回顾性分析2018年2月至2021年11月上海交通大学医学院附属瑞金医院收治的50例DLBCL/FL患者的临床病理资料。...目的 探讨初诊弥漫大B细胞淋巴瘤(DLBCL)并发滤泡淋巴瘤(FL)(DLBCL/FL)患者的临床病理特征、基因突变谱和预后影响因素。 方法 回顾性分析2018年2月至2021年11月上海交通大学医学院附属瑞金医院收治的50例DLBCL/FL患者的临床病理资料。采用靶向测序评估55个淋巴瘤相关基因的突变状态。总结患者临床特征,评估患者近期疗效;采用Kaplan-Meier法分析患者总生存(OS)和无进展生存(PFS);采用Cox比例风险模型评估OS和PFS的影响因素。 结果 50例DLBCL/FL患者中,男性23例(46%),国际预后指数(IPI)评分≥2分22例(44%),16例(32%)为双表达淋巴瘤(DEL),4例(8%)为双打击淋巴瘤(DHL)。一线治疗后患者的完全缓解(CR)率为68%(34/50),总有效率为78%(39/50)。中位随访23.3个月(5.1~50.9个月),2年OS率为82.1%,2年PFS率为67.1%,中位OS和PFS均未达到。靶向测序显示,KMT2D、MYD88、TP53、BTG2、DTX1、EZH2、CD70、CREBBP、DUSP2、HIST1H1C、HIST1H1E和PRDM1基因的突变频率均超过15%。多因素Cox回归分析显示,男性( HR =4.264,95% CI 1.144~15.896, P =0.031)和IPI评分≥2分( HR =6.800,95% CI 1.771-37.741, P =0.007)是初诊DLBCL/FL患者PFS的独立危险因素,TP53突变( HR =4.992,95% CI 1.027~24.258, P =0.046)是OS的独立危险因素。 结论 DLBCL/FL患者中男女比例接近,DHL患者较少。KMT2D、MYD88、TP53等多种基因的突变在DLBCL/FL患者中较为常见。DLBCL/FL患者整体治疗反应性较高,且预后较好。男性和IPI评分≥2分是DLBCL/FL患者PFS的独立危险因素,TP53突变是OS的独立危险因素。展开更多
文摘对锦屏深地核天体物理实验JUNA(Jinping Underground laboratory for Nuclear Astro-physics)离子源控制系统进行了研究,采用分布式系统模型构建。硬件采用PLC、串口服务器、伺服电机及工控机等部件实现了离子源设备的远程监测及控制。软件通过建立EPICS IOC运行时数据库,实现了对所有被控设备的集成。用户操作界面采用CSS(Control System Studio)开发,实现了操作人员对所有被控设备的透明访问。基于安全连锁规则设计了机器保护系统,实现了运行异常下的连锁保护。该控制系统应用于国内首套深地实验强流ECR离子源,运行稳定可靠,完全满足JUNA运行及物理实验的需求。
基金the National Natural Science Foundation of China(91850202,92150301,12074121,62105101,62175066,11727810,12034008,12274129,12274139)Science and Technology Commission of Shanghai Municipality(21XD1400900,20ZR1417100,21JM0010700).
文摘Various super-resolution microscopy techniques have been presented to explore fine structures of biological specimens.However,the super-resolution capability is often achieved at the expense of reducing imaging speed by either point scanning or multiframe computation.The contradiction between spatial resolution and imaging speed seriously hampers the observation of high-speed dynamics of fine structures.To overcome this contradiction,here we propose and demonstrate a temporal compressive super-resolution microscopy(TCSRM)technique.This technique is to merge an enhanced temporal compressive microscopy and a deep-learning-based super-resolution image reconstruction,where the enhanced temporal compressive microscopy is utilized to improve the imaging speed,and the deep-learning-based super-resolution image reconstruction is used to realize the resolution enhancement.The high-speed super-resolution imaging ability of TCSRM with a frame rate of 1200 frames per second(fps)and spatial resolution of 100 nm is experimentally demonstrated by capturing the flowing fluorescent beads in microfluidic chip.Given the outstanding imaging performance with high-speed super-resolution,TCSRM provides a desired tool for the studies of high-speed dynamical behaviors in fine structures,especially in the biomedical field.
文摘目的 探讨初诊弥漫大B细胞淋巴瘤(DLBCL)并发滤泡淋巴瘤(FL)(DLBCL/FL)患者的临床病理特征、基因突变谱和预后影响因素。 方法 回顾性分析2018年2月至2021年11月上海交通大学医学院附属瑞金医院收治的50例DLBCL/FL患者的临床病理资料。采用靶向测序评估55个淋巴瘤相关基因的突变状态。总结患者临床特征,评估患者近期疗效;采用Kaplan-Meier法分析患者总生存(OS)和无进展生存(PFS);采用Cox比例风险模型评估OS和PFS的影响因素。 结果 50例DLBCL/FL患者中,男性23例(46%),国际预后指数(IPI)评分≥2分22例(44%),16例(32%)为双表达淋巴瘤(DEL),4例(8%)为双打击淋巴瘤(DHL)。一线治疗后患者的完全缓解(CR)率为68%(34/50),总有效率为78%(39/50)。中位随访23.3个月(5.1~50.9个月),2年OS率为82.1%,2年PFS率为67.1%,中位OS和PFS均未达到。靶向测序显示,KMT2D、MYD88、TP53、BTG2、DTX1、EZH2、CD70、CREBBP、DUSP2、HIST1H1C、HIST1H1E和PRDM1基因的突变频率均超过15%。多因素Cox回归分析显示,男性( HR =4.264,95% CI 1.144~15.896, P =0.031)和IPI评分≥2分( HR =6.800,95% CI 1.771-37.741, P =0.007)是初诊DLBCL/FL患者PFS的独立危险因素,TP53突变( HR =4.992,95% CI 1.027~24.258, P =0.046)是OS的独立危险因素。 结论 DLBCL/FL患者中男女比例接近,DHL患者较少。KMT2D、MYD88、TP53等多种基因的突变在DLBCL/FL患者中较为常见。DLBCL/FL患者整体治疗反应性较高,且预后较好。男性和IPI评分≥2分是DLBCL/FL患者PFS的独立危险因素,TP53突变是OS的独立危险因素。
