摘要
目的比较不同采收时间的黄芪质量,并修订《中华人民共和国药典》中黄芪含量测定指标。方法采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),以乙腈-纯水溶液为流动相,梯度洗脱,蒸发光散射检测器漂移管温度60℃,气压30 psi,增益800℃,流速1.0 ml/min,柱温30℃,进样量20μl检测黄芪皂苷类成分含量;以乙腈-0.2%甲酸水溶液为流动相,梯度洗脱,流速1.0 ml/min,检测波长260 nm,柱温30℃,进样量10μl,检测黄芪黄酮类成分含量。考察黄芪皂苷Ⅰ的提取方式和提取时间,并检测12批不同产地黄芪中黄芪皂苷Ⅰ含量,确定其作为黄芪含量测定指标的限定范围。依据2020年版《中华人民共和国药典》四部中的烘干法、总灰分测定法、冷浸法测定黄芪水分、总灰分、水溶性浸出物。结果不同产地春、秋采收的黄芪总皂苷含量差异不明显,总黄酮含量有明显差异。除H11外,其余各批次黄芪中黄芪皂苷Ⅰ含量≥0.05%,故拟定黄芪皂苷Ⅰ含量限定范围为≥0.05%。12批黄芪水分、总灰分、水溶性浸出物的结果均符合《中华人民共和国药典》黄芪项下的相关规定。结论以在秋季采收的黄芪活性成分含量较高,质量较佳。建议新版《中华人民共和国药典》可将黄芪项下含量测定指标中的黄芪甲苷修订为黄芪皂苷Ⅰ。
Objective To compare the quality of Astragali Radix at different harvest time;To revise the content determination indexes of Astragali Radix in Chinese Pharmacopoeia.Methods An Agilent Eclipse XDB-C18 column(4.6 mm×150 mm,5μm)was used for the determination of saponins with acetonitrile-water solution as mobile phase in a gradient mode.The drift tube temperature of ELSD was 60℃;the pressure was 30 psi;the gain was 800℃;the flow rate was 1.0 ml/min;the column temperature was 30℃;the injection volume was 20μl;the acetonitrile-0.2%formic acid solution was used as mobile phase for the determination of flavonoids in a gradient mode;the flow rate was 1.0 ml/min;the detection wavelength was 260 nm;the column temperature was 30℃;the 10μl was injected.The limited range as an indicator for determining Astragali Radix content was determined by investigating the extraction method and extraction time of AstragalosideⅠand detecting the content of AstragalosideⅠin 12 batches of Astragali Radix from different origins.The moisture,total ash,and water-soluble extracts in Astragali Radix were determined according to the drying method,total ash determination method,and cold soaking method in the four parts of Chinese Pharmacopoeia(2020 edition),respectively.Results The content of total saponins in Astragali Radix harvested in spring and autumn in different origins was not significantly different,but the content of total flavonoids was significantly different.Except for H11,the content of AstragalosideⅠin the other batches of Astragali Radix was≥0.05%,so the content limit of AstragalosideⅠwas proposed to be≥0.05%.The results of moisture,total ash and water-soluble extracts in the 12 batches of Astragali Radix all meet the requirements in the Chinese Pharmacopoeia.Conclusions Astragali Radix harvested in autumn is with higher content of active components and better quality.At the same time,this study can provide a reference that the new version of Chinese Pharmacopoeia can revise the AstragalosideⅣin the conte
作者
刘蓬蓬
史辑
张凡
聂紫璇
王阳明
朱慧
Liu Pengpeng;Shi Ji;Zhang Fan;Nie Zixuan;Wang Yangming;Zhu Hui(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Key Laboratory of Processing Technology and Principle,National Administration of Traditional Chinese Medicine,Technology Innovation Center for Traditional Chinese Medicine Processing of Liaoning Province,Dalian 116600,China)
出处
《国际中医中药杂志》
2023年第7期868-874,共7页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(82003945)
辽宁省自然科学基金项目(2020-BS-163)
道地中药材炮制技术与标准研究项目(2019169)
辽宁中医药大学招聘录用高层次人才科研启动基金项目(201911)。
