目的探究超声电导大承气汤对全身麻醉腹腔镜胃癌根治术患者术后恢复的影响。方法选取2015年3月至2018年5月行全身麻醉腹腔镜胃癌根治术的96例胃癌患者为研究对象,按照随机数字表法分为2组各48例,对照组行常规护理,观察组于对照组基础上...目的探究超声电导大承气汤对全身麻醉腹腔镜胃癌根治术患者术后恢复的影响。方法选取2015年3月至2018年5月行全身麻醉腹腔镜胃癌根治术的96例胃癌患者为研究对象,按照随机数字表法分为2组各48例,对照组行常规护理,观察组于对照组基础上行超声电导大承气汤干预,对比2组术后胃肠功能恢复情况、血清胃泌素(GAS)、血浆胃动素(MTL)水平以及40项恢复质量评分(QoR40)。结果观察组术后各项胃肠功能指标均优于对照组(P<0.05);与术前1 d比较,2组术后24 h GAS与MTL水平均降低,观察组GAS与MTL分别为(92.56±5.12)、(258.58±5.59)ng/L,高于对照组的(52.12±3.15)、(185.63±4.12)ng/L,差异有统计学意义(t值为46.608、72.782,P<0.01);与干预前比较,2组干预后QoR40评分均提高,观察组和对照组分别为(198.56±5.58)、(152.12±2.63)分,且观察组高于对照组,差异有统计学意义(t值为52.157,P<0.01)。结论超声电导大承气汤利于促进全身麻醉腹腔镜胃癌根治术患者术后恢复。展开更多
The mechanical properties and fracturing mechanism of shale containing beddings are critically important in shale gas exploitation and wellbore stability.To investigate the effects of shale bedding on crack behavior a...The mechanical properties and fracturing mechanism of shale containing beddings are critically important in shale gas exploitation and wellbore stability.To investigate the effects of shale bedding on crack behavior and fracturing mechanism,scanning electron microscope(SEM)with a loading system was employed to carry out three-point bending tests on Longmaxi outcrop shale.The crack initiation and propagation of Longmaxi shale were observed and recorded by taking photos during loading.The cracking paths were extracted to calculate the crack length through a MATLAB program.The peak load,fracture toughness and fracture energy all increase with the bedding angle from 0°to 90°.The crack length and energy were also found to increase with the bedding angle in the range of 0°-600 and then drop slightly.The fracturing mechanism of shale includes the main crack affected by the bedding angle and disturbed by randomly distributed particles.The main cracking path was accompanied by several microcrack branches which could form an interconnected crack system.When the main crack encounters larger sedimentary particles,it will deflect around the particles and then restore to the initial direction.A numerical technique using extended finite element method(XFEM)coupled with anisotropic cohesive damage criteria was developed,which is able to capture the dependence of crack propagations on bedding angle and sedimentary particles.This study sheds light on understanding and predicting mesoscale fracture behavior of shale with different bedding angles.展开更多
We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely el...We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely elusive.OsbZIP72 was expressed and integrated into rice transgenic plant genomes,and the OsbZIP72 transcript in overexpression lines was elicited by salinity,abscisic acid(ABA)and drought stresses.OsbZIP72 overexpressing plants showed higher tolerance to drought and salinity stresses,while knock-out transgenic lines showed higher sensitivity to these stresses.The differentially expressed genes(DEGs)from RNA-sequencing data encompassed several abiotic stress genes,and the functional classification of these DEGs demonstrated the robust transcriptome diversity in OsbZIP72.Yeast one-hybrid,along with luciferase assay,indicated that OsbZIP72 acted as a transcriptional initiator.Remarkably,electrophoresis mobility assay revealed that OsbZIP72 bound directly to the ABAresponsive element in the OsHKT1;1 promoter region and activated its transcription.Overall,our findings revealed that OsbZIP72 can act as a transcriptional modulator with the ability to induce the expression of OsHKT1;1 in response to environmental stress through an ABA-dependent regulatory pathway,indicating that OsbZIP72 can play a crucial role in the ABA-mediated salt and drought tolerance pathway in rice.展开更多
文摘目的探究超声电导大承气汤对全身麻醉腹腔镜胃癌根治术患者术后恢复的影响。方法选取2015年3月至2018年5月行全身麻醉腹腔镜胃癌根治术的96例胃癌患者为研究对象,按照随机数字表法分为2组各48例,对照组行常规护理,观察组于对照组基础上行超声电导大承气汤干预,对比2组术后胃肠功能恢复情况、血清胃泌素(GAS)、血浆胃动素(MTL)水平以及40项恢复质量评分(QoR40)。结果观察组术后各项胃肠功能指标均优于对照组(P<0.05);与术前1 d比较,2组术后24 h GAS与MTL水平均降低,观察组GAS与MTL分别为(92.56±5.12)、(258.58±5.59)ng/L,高于对照组的(52.12±3.15)、(185.63±4.12)ng/L,差异有统计学意义(t值为46.608、72.782,P<0.01);与干预前比较,2组干预后QoR40评分均提高,观察组和对照组分别为(198.56±5.58)、(152.12±2.63)分,且观察组高于对照组,差异有统计学意义(t值为52.157,P<0.01)。结论超声电导大承气汤利于促进全身麻醉腹腔镜胃癌根治术患者术后恢复。
基金financially supported by National Natural Science Foundation of China(grant No.41877257)Beijing OutstandingYoung Scientist Program(Grant No.BJJWZYJH01201911413037)+1 种基金Shaanxi Coal Group Key Project(Grant No.2018SMHKJA-J-03)Yueqi outstanding scholar Award Program by China University of Mining and Technology(Beijing),China。
文摘The mechanical properties and fracturing mechanism of shale containing beddings are critically important in shale gas exploitation and wellbore stability.To investigate the effects of shale bedding on crack behavior and fracturing mechanism,scanning electron microscope(SEM)with a loading system was employed to carry out three-point bending tests on Longmaxi outcrop shale.The crack initiation and propagation of Longmaxi shale were observed and recorded by taking photos during loading.The cracking paths were extracted to calculate the crack length through a MATLAB program.The peak load,fracture toughness and fracture energy all increase with the bedding angle from 0°to 90°.The crack length and energy were also found to increase with the bedding angle in the range of 0°-600 and then drop slightly.The fracturing mechanism of shale includes the main crack affected by the bedding angle and disturbed by randomly distributed particles.The main cracking path was accompanied by several microcrack branches which could form an interconnected crack system.When the main crack encounters larger sedimentary particles,it will deflect around the particles and then restore to the initial direction.A numerical technique using extended finite element method(XFEM)coupled with anisotropic cohesive damage criteria was developed,which is able to capture the dependence of crack propagations on bedding angle and sedimentary particles.This study sheds light on understanding and predicting mesoscale fracture behavior of shale with different bedding angles.
基金supported by the earmarked funds for China Agriculture Research System(Grant No.CARS-01-61)National Science and Technology Support Program of China(Grant No.2015BAD01B01)+3 种基金Science and Technology Support Program of Jiangsu Province,China(Grant Nos.BE2016370-3 and BE2017323)the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20161299)the Financial Grant Support Program of Lianyungang City,Jiangsu Province,China(Grant Nos.QNJJ1704 and QNJJ1912)National Natural Science Foundation of China(Grant No.31701395).
文摘We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely elusive.OsbZIP72 was expressed and integrated into rice transgenic plant genomes,and the OsbZIP72 transcript in overexpression lines was elicited by salinity,abscisic acid(ABA)and drought stresses.OsbZIP72 overexpressing plants showed higher tolerance to drought and salinity stresses,while knock-out transgenic lines showed higher sensitivity to these stresses.The differentially expressed genes(DEGs)from RNA-sequencing data encompassed several abiotic stress genes,and the functional classification of these DEGs demonstrated the robust transcriptome diversity in OsbZIP72.Yeast one-hybrid,along with luciferase assay,indicated that OsbZIP72 acted as a transcriptional initiator.Remarkably,electrophoresis mobility assay revealed that OsbZIP72 bound directly to the ABAresponsive element in the OsHKT1;1 promoter region and activated its transcription.Overall,our findings revealed that OsbZIP72 can act as a transcriptional modulator with the ability to induce the expression of OsHKT1;1 in response to environmental stress through an ABA-dependent regulatory pathway,indicating that OsbZIP72 can play a crucial role in the ABA-mediated salt and drought tolerance pathway in rice.
