Objective To investigate the effect of emodin on high glucose(HG)-induced podocyte apoptosis and whether the potential anti-apoptotic mechanism of emodin is related to induction of adenosine-monophosphate-activated pr...Objective To investigate the effect of emodin on high glucose(HG)-induced podocyte apoptosis and whether the potential anti-apoptotic mechanism of emodin is related to induction of adenosine-monophosphate-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)-mediated autophagy in podocytes(MPC5 cells)in vitro.Methods MPC5 cells were treated with different concentrations of HG(2.5,5,10,20,40,80 and 160 mmol/L),emodin(2,4,8µmol/L),or HG(40 mmol/L)and emodin(4µmol/L)with or without rapamycin(Rap,100 nmol/L)and compound C(10µmol/L).The viability and apoptosis of MPC5 cells were detected using cell counting kit-8(CCK-8)assay and flow cytometry analysis,respectively.The expression levels of cleaved caspase-3,autophagy marker light chain 3(LC3)Ⅰ/Ⅱ,and AMPK/mTOR signaling pathway-related proteins were determined by Western blot.The changes of morphology and RFP-LC3 fluorescence were observed under microscopy.Results HG at 20,40,80 and 160 mmol/L dose-dependently induced cell apoptosis in MPC5 cells,whereas emodin(4µmol/L)significantly ameliorated HG-induced cell apoptosis and caspase-3 cleavage(P<0.01).Emodin(4µmol/L)significantly increased LC3-Ⅱ protein expression levels and induced RFP-LC3-containing punctate structures in MPC5 cells(P<0.01).Furthermore,the protective effects of emodin were mimicked by rapamycin(100 nmol/L).Moreover,emodin increased the phosphorylation of AMPK and suppressed the phosphorylation of mTOR.The AMPK inhibitor compound C(10µmol/L)reversed emodin-induced autophagy activation.Conclusion Emodin ameliorated HG-induced apoptosis of MPC5 cells in vitro that involved induction of autophagy through the AMPK/mTOR signaling pathway,which might provide a potential therapeutic option for diabetic nephropathy.展开更多
Ninety cases of scapulohumeral periarthritis were treated by electroacupuncture plus Tuina and the effective rate was 94.4%. The effective rate was 81.9% in the control group (treated by Frozen Shoulder Decoction adde...Ninety cases of scapulohumeral periarthritis were treated by electroacupuncture plus Tuina and the effective rate was 94.4%. The effective rate was 81.9% in the control group (treated by Frozen Shoulder Decoction added by other herbal drugs) of 83 cases. The result indicated that the treatments of the two groups were effective, but the therapeutic effect of the treatment group was better than that of the control group. In the treatment group, the cure rate was higher, the duration of treatment was shorter and the sequelae of conglutination and rigidity of the shoulder joint, muscular atrophy and so on were reduced. Key Words Electroacupuncture - Tuina - Massage - Inflammation - Shoulder Joint Translator: KUAI Le展开更多
以工厂胀罐酱油为研究对象,对导致酱油胀罐的产气菌进行分离和鉴定。使用高盐MRS培养基(含6%Na Cl)分离到一种厌氧菌,经产气实验证明该菌是导致酱油产气胀罐的原因,经16S r DNA基因测序以及高通量测序确证该厌氧产气菌为破布子乳酸杆菌(...以工厂胀罐酱油为研究对象,对导致酱油胀罐的产气菌进行分离和鉴定。使用高盐MRS培养基(含6%Na Cl)分离到一种厌氧菌,经产气实验证明该菌是导致酱油产气胀罐的原因,经16S r DNA基因测序以及高通量测序确证该厌氧产气菌为破布子乳酸杆菌(Lactobacillus pobuzihii);采用高盐PDA培养基没有分离到酵母菌,且用多对18S rRNA引物对提取的基因组DNA进行PCR扩增阴性,证明酱油胀罐的原因为污染了破布子乳酸菌且没有酵母菌或其他真菌污染。该研究显示了基因测序方法对于食品中未知、难培养微生物污染的检测具有优越性。展开更多
基金Supported by the Chinese Medicine Research Project of Hubei Provincial Health Commission(No.ZY2019Q024)Scientific Research Project of Wuhan Municipal Health Commission(No.WX20B11)Scientific Research Project of Wuhan Municipal Health Commission(No.WZ20C01)。
文摘Objective To investigate the effect of emodin on high glucose(HG)-induced podocyte apoptosis and whether the potential anti-apoptotic mechanism of emodin is related to induction of adenosine-monophosphate-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)-mediated autophagy in podocytes(MPC5 cells)in vitro.Methods MPC5 cells were treated with different concentrations of HG(2.5,5,10,20,40,80 and 160 mmol/L),emodin(2,4,8µmol/L),or HG(40 mmol/L)and emodin(4µmol/L)with or without rapamycin(Rap,100 nmol/L)and compound C(10µmol/L).The viability and apoptosis of MPC5 cells were detected using cell counting kit-8(CCK-8)assay and flow cytometry analysis,respectively.The expression levels of cleaved caspase-3,autophagy marker light chain 3(LC3)Ⅰ/Ⅱ,and AMPK/mTOR signaling pathway-related proteins were determined by Western blot.The changes of morphology and RFP-LC3 fluorescence were observed under microscopy.Results HG at 20,40,80 and 160 mmol/L dose-dependently induced cell apoptosis in MPC5 cells,whereas emodin(4µmol/L)significantly ameliorated HG-induced cell apoptosis and caspase-3 cleavage(P<0.01).Emodin(4µmol/L)significantly increased LC3-Ⅱ protein expression levels and induced RFP-LC3-containing punctate structures in MPC5 cells(P<0.01).Furthermore,the protective effects of emodin were mimicked by rapamycin(100 nmol/L).Moreover,emodin increased the phosphorylation of AMPK and suppressed the phosphorylation of mTOR.The AMPK inhibitor compound C(10µmol/L)reversed emodin-induced autophagy activation.Conclusion Emodin ameliorated HG-induced apoptosis of MPC5 cells in vitro that involved induction of autophagy through the AMPK/mTOR signaling pathway,which might provide a potential therapeutic option for diabetic nephropathy.
文摘Ninety cases of scapulohumeral periarthritis were treated by electroacupuncture plus Tuina and the effective rate was 94.4%. The effective rate was 81.9% in the control group (treated by Frozen Shoulder Decoction added by other herbal drugs) of 83 cases. The result indicated that the treatments of the two groups were effective, but the therapeutic effect of the treatment group was better than that of the control group. In the treatment group, the cure rate was higher, the duration of treatment was shorter and the sequelae of conglutination and rigidity of the shoulder joint, muscular atrophy and so on were reduced. Key Words Electroacupuncture - Tuina - Massage - Inflammation - Shoulder Joint Translator: KUAI Le
