Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial ...Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial with blinding of participants,outcome assessment and statistician.Setting:acupuncture-Moxibustion,Tuina and Orthopedics Department and the Orthopedics Department of Shanghai the Sixth People’s Hospital,June 2018 to January 2021.Participants:a total of 64 patients with iliotibial band friction syndrome(ITBS).Interventions:with a 1:1 ratio the patients were randomly assigned to either the conventional acupuncture group(CA,n=32) or the Relaxing Needling with Sharp Long Needle group(RNSLN,n=32).The CA group was treated by applying acupuncture at Jūliáo(居髎GB 29),Fēngshì(风市GB 31),Liángqiū(梁丘ST34),Yánglingquán(阳陵泉GB 34),and Ashi points(阿是穴)of the affected side,and the RNSLN group was treated by applying relaxing needling with sharp long needle at GB 29,ST 34,and Ashi points of the affected side.Both groups were treated once every other day,10 times as one course of treatment.Outcome measures:the treatment effect was evaluated after one course of treatment by comparing their KSS scores and VAS scores,as well as overall response rate.Results:Sixty-four patients were included in the final data analysis.There was no difference in KSS and VAS scores between the two groups before treatment(P>0.05).After treatment,the KSS score of the two groups was higher than that before treatment,the VAS score of the two groups was lower than that before treatment(P<0.05).The KSS score of the RNSLN group was higher than that of the CA group,and the VAS score of the RNSLN group was lower than that of the CA group(P<0.05).The overall response rate of the RNSLN group was 93.75%,higher than 75% of the control group(P<0.05).Each group has 1case of subcutaneous bleeding as adverse event.Conclusion:Relaxing Needling with Sharp Long Needle could be more beneficial for iliotibial band friction syndrome(ITBS展开更多
酶催化交联是蛋白质改性的常用加工手段,交联方式和交联位点不同,对蛋白质性质的影响也不同。采用转谷氨酰胺酶(microbial transglutaminase,MTG)催化Ara h 2发生交联反应。通过液相-质谱联用对交联后的蛋白产物进行分析,再利用StavroX...酶催化交联是蛋白质改性的常用加工手段,交联方式和交联位点不同,对蛋白质性质的影响也不同。采用转谷氨酰胺酶(microbial transglutaminase,MTG)催化Ara h 2发生交联反应。通过液相-质谱联用对交联后的蛋白产物进行分析,再利用StavroX软件分析质谱数据,寻找不同反应条件下,蛋白质交联反应的发生位点。结果显示,在MTG的催化下,K142、K52均参与交联反应。还原后的Ara h 2的交联反应较未还原蛋白更为活跃,未还原蛋白中仅发现3对分子内交联肽段,而还原后的蛋白中找到16对交联肽段(既包括分子内交联也包括分子间交联),但还原蛋白中仅K142参与了分子内交联反应。研究揭示的Ara h 2交联方式和交联位点,为交联反应对蛋白质改性机制研究奠定了基础。展开更多
基金Supported by Shanghai Three-year Action Plan for Further Accelerating the Development of Traditional Chinese Medicine (2018-2020):ZY (2018-2020)-ZYJS-09Shanghai Three-year Action Plan for Further Accelerating the Development of Traditional Chinese Medicine (2018-2020):No.ZY(2018-2020)-FWTX-4020Shanghai Three-year Action Plan for Further Accelerating the Inheritance,Innovation,Development of Traditional Chinese Medicine (2021-2023):No.ZY (2021-2023)-0205-04。
文摘Objective:to observe the clinical effect of acupuncture by mángzhen huid(relaxing needling with sharp long needle) on iliotibial band friction syndrome(ITBS).Design:this was a two-arm randomized controlled trial with blinding of participants,outcome assessment and statistician.Setting:acupuncture-Moxibustion,Tuina and Orthopedics Department and the Orthopedics Department of Shanghai the Sixth People’s Hospital,June 2018 to January 2021.Participants:a total of 64 patients with iliotibial band friction syndrome(ITBS).Interventions:with a 1:1 ratio the patients were randomly assigned to either the conventional acupuncture group(CA,n=32) or the Relaxing Needling with Sharp Long Needle group(RNSLN,n=32).The CA group was treated by applying acupuncture at Jūliáo(居髎GB 29),Fēngshì(风市GB 31),Liángqiū(梁丘ST34),Yánglingquán(阳陵泉GB 34),and Ashi points(阿是穴)of the affected side,and the RNSLN group was treated by applying relaxing needling with sharp long needle at GB 29,ST 34,and Ashi points of the affected side.Both groups were treated once every other day,10 times as one course of treatment.Outcome measures:the treatment effect was evaluated after one course of treatment by comparing their KSS scores and VAS scores,as well as overall response rate.Results:Sixty-four patients were included in the final data analysis.There was no difference in KSS and VAS scores between the two groups before treatment(P>0.05).After treatment,the KSS score of the two groups was higher than that before treatment,the VAS score of the two groups was lower than that before treatment(P<0.05).The KSS score of the RNSLN group was higher than that of the CA group,and the VAS score of the RNSLN group was lower than that of the CA group(P<0.05).The overall response rate of the RNSLN group was 93.75%,higher than 75% of the control group(P<0.05).Each group has 1case of subcutaneous bleeding as adverse event.Conclusion:Relaxing Needling with Sharp Long Needle could be more beneficial for iliotibial band friction syndrome(ITBS
文摘酶催化交联是蛋白质改性的常用加工手段,交联方式和交联位点不同,对蛋白质性质的影响也不同。采用转谷氨酰胺酶(microbial transglutaminase,MTG)催化Ara h 2发生交联反应。通过液相-质谱联用对交联后的蛋白产物进行分析,再利用StavroX软件分析质谱数据,寻找不同反应条件下,蛋白质交联反应的发生位点。结果显示,在MTG的催化下,K142、K52均参与交联反应。还原后的Ara h 2的交联反应较未还原蛋白更为活跃,未还原蛋白中仅发现3对分子内交联肽段,而还原后的蛋白中找到16对交联肽段(既包括分子内交联也包括分子间交联),但还原蛋白中仅K142参与了分子内交联反应。研究揭示的Ara h 2交联方式和交联位点,为交联反应对蛋白质改性机制研究奠定了基础。
