MYB-type transcription factors contain the conserved MYB DNA-binding domain of approximately 50 amino acids and are involved in the regulation of many aspects of plant growth, development, metabolism and stress respon...MYB-type transcription factors contain the conserved MYB DNA-binding domain of approximately 50 amino acids and are involved in the regulation of many aspects of plant growth, development, metabolism and stress responses. From soybean plants, we identified 156 GmMYB genes using our previously obtained 206 MYB unigenes, and 48 were found to have full-length open-reading frames. Expressions of all these identified genes were examined, and we found that expressions of 43 genes were changed upon treatment with ABA, salt, drought and/or cold stress. Three GmMYB genes, GmMYB76, GmMYB92 and GmMYB177, were chosen for further analysis. Using the yeast assay system, GmMYB76 and GmMYB92 were found to have transactivation activity and can form homodimers. GmMYB177 did not appear to have transactivation activity but can form heterodimers with GmMYB76. Yeast onehybrid assay revealed that all the three GmMYBs could bind to cis-elements TAT AAC GGT TTT TT and CCG GAA AAA AGG AT, but with different affinity, and GmMYB92 could also bind to TCT CAC CTA CC. The transgenic Arabidopsis plants overexpressing GmMYB 76 or GmMYB177 showed better performance than the GmMYB92-transgenic plants in salt and freezing tolerance. However, these transgenic plants exhibited reduced sensitivity to ABA treatment at germination stage in comparison with the wild-type plants. The three GmMYB genes differentially affected a subset of stress-responsive genes in addition to their regulation of a common subset of stress-responsive genes. These resuits indicate that the three GmMYB genes may play differential roles in stress tolerance, possibly through regulation of stress-responsive genes.展开更多
The terahertz region is a special region of the electromagnetic spectrum that incorporates the advantages of both microwaves and infrared light waves.In the past decade,metamaterials with effective medium parameters o...The terahertz region is a special region of the electromagnetic spectrum that incorporates the advantages of both microwaves and infrared light waves.In the past decade,metamaterials with effective medium parameters or gradient phases have been studied to control terahertz waves and realize functional devices.Here,we present a new approach to manipulate terahertz waves by using coding metasurfaces that are composed of digital coding elements.We propose a general coding unit based on a Minkowski closed-loop particle that is capable of generating 1-bit coding(with two phase states of 0 and 180°),2-bit coding(with four phase states of 0,90°,180°,and 270°),and multi-bit coding elements in the terahertz frequencies by using different geometric scales.We show that multi-bit coding metasurfaces have strong abilities to control terahertz waves by designing-specific coding sequences.As an application,we demonstrate a new scattering strategy of terahertz waves—broadband and wide-angle diffusion—using a 2-bit coding metasurface with a special coding design and verify it by both numerical simulations and experiments.The presented method opens a new route to reducing the scattering of terahertz waves.展开更多
Ginkgo biloba is grown worldwide as an ornamental plant for its golden leaf color.However,the regulatory mechanism of leaf coloration in G.biloba remains unclear.Here,we compared G.biloba gold-colored mutant leaves an...Ginkgo biloba is grown worldwide as an ornamental plant for its golden leaf color.However,the regulatory mechanism of leaf coloration in G.biloba remains unclear.Here,we compared G.biloba gold-colored mutant leaves and normal green leaves in cytological,physiological and transcriptomic terms.We found that chloroplasts of the mutant were fewer and smaller,and exhibited ruptured thylakoid membranes,indistinct stromal lamellae and irregularly arranged vesicles.Physiological experiments also showed that the mutant had a lower chlorophyll,lower flavonoid and higher carotenoid contents(especially lutein).We further used transcriptomic sequencing to identify 116 differentially expressed genes(DEGs)and 46 transcription factors(TFs)involved in chloroplast development,chlorophyll metabolism,pigment biosynthesis and photosynthesis.Among these,the chlorophyll biosynthesis-related PPO showed down-regulation,while chlorophyll degradation-related NYC/NOL had up-regulated expression in mutant leaves.Z-ISO,ZDS,and LCYE,which are involved in carotenoid biosynthesis were up-regulated.Quantitative real-time PCR(RT-qPCR)further confirmed the altered expression levels of these genes at three stages.The alteration of PPO and NYC/NOL gene expression might affect chlorophyll biosynthesis and promote degradation of chlorophyll b to chlorophyll a,while the up-regulated genes Z-ISO,ZDS and LCYE enhanced carotenoid accumulation.Consequently,changes in the ratio of carotenoids to chlorophylls were the main factors driving the golden leaf coloration in the mutant G.biloba.展开更多
Background Photodynamic therapy （PDT） with 5-aminolevulinic acid （ALA） is an emerging technique for the treatment of genital human papillomavirus （HPV）-induced benign and premalignant lesions. We report here in ...Background Photodynamic therapy （PDT） with 5-aminolevulinic acid （ALA） is an emerging technique for the treatment of genital human papillomavirus （HPV）-induced benign and premalignant lesions. We report here in a case series of condyloma acuminata （CA） in pregnancy successfully treated with ALA-PDT. Methods Five pregnant patients with CA received three to four times treatment respectively. Patients were followed up for 6-23 months after treatment. Results The clearance rate of genital warts was 100%. No recurrence was found during the follow-up period. Major adverse events reported were mild erosion, pain, and local edema. All pregnancies resulted in healthy live births without delivery complications. Conclusions PDT with topical ALA seems to be safe and effective in the treatment of CA in pregnancy. It demonstrated high clearance rate of warts, was well-tolerated by patients, and showed no adverse effects on mothers or fetuses. ALA-PDT may be an ideal strategy of treatment for pregnant women with CA.展开更多
Ethylene as a gas phytohormone plays significant roles in the whole life cycle of plants, ranging from growth and development to stress responses. A linear ethylene signaling pathway has been established in the dicoty...Ethylene as a gas phytohormone plays significant roles in the whole life cycle of plants, ranging from growth and development to stress responses. A linear ethylene signaling pathway has been established in the dicotyledonous model plant Arabidopsis. However, the ethylene signaling mechanism in monocotyledonous plants such as rice is largely unclear. In this review, We compare the ethylene response phenotypes of dark-grown seedlings of Arabidopsis, rice, and other monocotyledonous plants （maize, wheat, sorghum, and Brachypodium distachyon） and pinpoint that rice has a distinct phenotype of root inhibition but coleoptile promotion in etiolated seedlings upon ethylene treatment. We further summarize the homologous genes of Arabidopsis ethylene signaling components in these monocotyledonous plants and discuss recent progress. Although conserved in most aspects, ethylene signaling in rice has evolved new features compared with that in Arabidopsis. These analyses provide novel insights into the understanding of ethylene signaling in the dicotyledonous Arabidopsis and monocotyledonous plants, particularly rice. Further characterization of rice ethylene-responsive mutants and their corresponding genes will help us better understand the whole picture of ethylene signaling mechanisms in plants.展开更多
Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In this study, through w...Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In this study, through whole-genome sequencing of a recombinant inbred line population derived from a cross between a wild soybean ZYD7 and a cultivated soybean HN44, and mapping of quantitative trait loci for seed weight, we discovered that a phosphatase 2C-1 （PP2C-1） allele from wild soybean ZYD7 contributes to the increase in seed weight/size. PP2C-1 may achieve this function by enhancing cell size of integument and activating a subset of seed trait-related genes. We found that PP2C-1 is associ- ated with GmBZR1, a soybean ortholog of Arabidopsis BZR1, one of key transcription factors in brassi- nosteroid （BR） signaling, and facilitate accumulation of dephosphorylated GmBZR1. In contrast, the PP2C-2 allele with variations of a few amino acids at the N-terminus did not exhibit this function. More- over, we showed that GmBZR1 could promote seed weight/size in transgenic plants. Through analysis of cultivated soybean accessions, we found that 40% of the examined accessions do not have the PP2C-1 allele, suggesting that these accessions can be improved by introduction of this allele. Taken together, our study identifies an elite allele PP2C-1, which can enhance seed weight and/or size in soybean, and pinpoints that manipulation of this allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.展开更多
Several studies have demonstrated that the Chinese herb Gastrodia elata Blume can protect against amyloid beta-peptide （Ap）-induced cell death. To investigate the possible therapeutic effects of Gastrodia elata Blum...Several studies have demonstrated that the Chinese herb Gastrodia elata Blume can protect against amyloid beta-peptide （Ap）-induced cell death. To investigate the possible therapeutic effects of Gastrodia elata Blume on Alzheimer＇s disease, we established a rat model of AIzheimer＇s disease by injecting A325-35 into bilateral hippocampi. These rats were intragastrically administered 500 or 1 000 mg/kg Gastrodia elata Blume per day for 52 consecutive days. Morris water maze tests showed that Gastrodia elata Blume treatment significantly improved the spatial memory of Alzheimer＇s disease rats. Congo red staining revealed that Gastrodia elata Blume significantly reduced the number of amyloid deposits in the hippocampus of these rats. Western blot analysis showed that choline acetyltransferase expression in the medial septum and hippocampus was significantly increased by the treatment of Gastrodia elata Blume, while EIIman method showed significant decrease in the activity of acetylcholinesterase in all three regions （prefrontal cortex, medial septum and hippocampus）. These findings suggest that long-term administration of Gastrodia elata Blume has therapeutic potential for Alzheimer＇s disease.展开更多
We demonstrate a precision measurement of local gravity acceleration 9 in Wuhan by a compact cold atom interferometer. The atom interferometer is in vertical Mach-Zehnder configuration realized using a 7r /2 - π - π...We demonstrate a precision measurement of local gravity acceleration 9 in Wuhan by a compact cold atom interferometer. The atom interferometer is in vertical Mach-Zehnder configuration realized using a 7r /2 - π - π /2 Raman pulse sequence. Cold atoms were prepared in a magneto-optical trap, launched upward to form an atom fountain, and then coherently manipulated to interfere by stimulated Raman transition. Population signal vs Raman laser phase was recorded as interference fringes, and the local gravity was deduced from the interference signal. We have obtained a resolution of 7 × 10-9g after an integration time of 236s under the best vibrational environment conditions. The absolute g value was derived from the chirp rate with a difference of 1.5 × 10--07 g compared to the gravity reference value. The tidal phenomenon was observed by continuously monitoring the local gravity over 123 h.展开更多
Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and character...Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. Manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.展开更多
AIM: To evaluate the reliability and accuracy of threedimensional(3D) reconstruction for liver resection in patients with hepatic alveolar echinococcosis(HAE).METHODS: One-hundred and six consecutive patients with HAE...AIM: To evaluate the reliability and accuracy of threedimensional(3D) reconstruction for liver resection in patients with hepatic alveolar echinococcosis(HAE).METHODS: One-hundred and six consecutive patients with HAE underwent hepatectomy at our hospital between May 2011 and January 2015. Fifty-nine patients underwent preoperative 3D reconstruction and "virtual" 3D liver resection before surgery(Group A). Another 47 patients used conventional imaging methods for preoperative assessment(Group B). Outcomes of hepatectomy were compared between the two groups.RESULTS: There was no significant difference in preoperative data between the two groups. Compared with patients in Group B, those in Group A had a significantly shorter operation time(227.1 ± 51.4 vs 304.6 ± 88.1 min; P < 0.05), less intraoperative blood loss(308.1 ± 135.4 vs 458.1 ± 175.4 m L; P < 0.05), and lower requirement for intraoperative blood transfusion(186.4 ± 169.6 vs 289.4 ± 199.2 m L; P < 0.05). Estimated resection liver volumes in bothgroups had good correlation with actual graft weight(Group A: r = 0.978; Group B: r = 0.960). There was a significant higher serum level of albumin in Group A(26.3 ± 5.9 vs 22.6 ± 4.3 g/L, P < 0.05). Other postoperative laboratory parameters(serum levels of aminotransferase and bilirubin; prothrombin time) and duration of postoperative hospital stay were similar. Sixteen complications occurred in Group A and 19 in Group B. All patients were followed for 3-46(mean, 17.3) mo. There was no recurrence of lesions in Group A, but two recurrences in Group B. There were three deaths: two from cerebrovascular accident, and one from car accident.CONCLUSION: 3D reconstruction provides comprehensive and precise anatomical information for the liver. It also improves the chance of success and reduces the risk of hepatectomy in HAE.展开更多
MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division proce...MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division process remains unknown. Here, we report that an R2R3-type transcription factor gene, AtMYB59, is involved in the regulation of cell cycle progression and root growth. The AtMYB59 protein is localized in the nuclei of onion epidermal cells and has transactivation activity. Expression of AtMYB59 in yeast cells suppresses cell proliferation, and the transfor- mants have more nuclei and higher anenpioid DNA content with longer cells. Mutation in the conserved domain of AtMYB59 abolishes its effects on yeast cell growth. In synchronized Arabidopsis cell suspensions, the AtMYB59 gene is specifically expressed in the S phase during cell cycle progression. Expression and promoter-GUS analysis reveals that the AtMYB59 gene is abundantly expressed in roots. Transgenic plants overexpressing AtMYB59 have shorter roots compared with wild-type plants （Arabidopsis accession Col-0）, and around half of the mitotic cells in root tips are at metaphase. Conversely, the null mutant myb59-1 has longer roots and fewer mitotic cells at metaphase than Col, suggesting that AtMYB59 may inhibit root growth by extending the metaphase of mitotic cells. AtMYB59 regulates many downstream genes, including the CYCB1;1 gene, probably through binding to MYB-responsive elements. These results support a role forAtMYB59 in cell cycle regulation and plant root growth.展开更多
目的】建立苹果原料制干适宜性评价模型,实现基于苹果原料指标预测干制品品质的目标,为苹果制干专用化原料的筛选提供方法依据,为明确苹果干制品品质形成的基础物质提供数据支持。【方法】以来自7个不同主产区的21个主栽品种,共34份苹...目的】建立苹果原料制干适宜性评价模型,实现基于苹果原料指标预测干制品品质的目标,为苹果制干专用化原料的筛选提供方法依据,为明确苹果干制品品质形成的基础物质提供数据支持。【方法】以来自7个不同主产区的21个主栽品种,共34份苹果鲜果样本为研究对象,运用多种数据处理方法建立苹果脆片品质综合评价模型与苹果原料制干适宜性评价模型。(1)利用压差闪蒸干燥方法制备34份苹果鲜果的脆片样本,测定苹果脆片17项品质指标,采用因子分析进行降维并筛选得到苹果脆片品质评价核心指标,运用层次分析法得到脆片核心指标权重值,构建脆片品质综合评价模型并计算得到脆片综合评价得分。(2)测定34份苹果鲜果样本22项品质指标,与脆片核心指标进行相关性分析并筛选出与脆片品质相关的果实特征指标。选用29个样本以果实特征指标为输入,对应脆片综合评价得分为输出,利用误差反向传播(Error Back Propagation,BP)神经网络算法构建学习模型;其余5个样本为验证样本,评价学习模型的预测准确性。变换3组学习样本构建3个学习模型,对比3个模型的预测准确性,验证建模方法的合理性与稳定性。【结果】苹果脆片L*值、脆度、膨化度、可滴定酸含量、可溶性糖含量和粗蛋白含量被确定为不同样本脆片品质综合评价的核心指标,构建的苹果脆片品质综合评价模型为Y_(综合得分)=L*值×0.3724+脆度×0.2665+膨化度×0.1583+可滴定酸含量×0.0890+可溶性糖含量×0.0569+粗蛋白含量×0.0569。34个苹果鲜果样本制得的脆片综合得分范围为0.2069—0.7933,存在较大差异,得分排名前3的苹果样本为‘辽宁华红’‘辽宁华金’和‘山东烟富6号’,排名最后的苹果样本为‘陕西秦冠’。基于脆片核心指标与苹果果实品质指标相关性分析结果,筛选出苹果果实的果形指数、果肉a*值、pH、展开更多
Versatile devices,especially tunable ones,for terahertz imaging,sensing and high-speed communication,are in high demand.Liquid crystal based components are perfect candidates in the optical range;however,they encounte...Versatile devices,especially tunable ones,for terahertz imaging,sensing and high-speed communication,are in high demand.Liquid crystal based components are perfect candidates in the optical range;however,they encounter significant challenges in the terahertz band,particularly the lack of highly transparent electrodes and the drawbacks induced by a thick cell.Here,a strategy to overcome all these challenges is proposed:Few-layer porous graphene is employed as an electrode with a transmittance of more than 98%.A subwavelength metal wire grid is utilized as an integrated high-efficiency electrode and polarizer.The homogeneous alignment of a high-birefringence liquid crystal is implemented on both frail electrodes via a non-contact photo-alignment technique.A tunable terahertz waveplate is thus obtained.Its polarization evolution is directly demonstrated.Furthermore,quarter-wave plates that are electrically controllable over the entire testing range are achieved by stacking two cells.The proposed solution may pave a simple and bright road toward the development of various liquid crystal terahertz apparatuses.展开更多
This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or a...This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or an end car, and Vehicle TP03, the third car counting from TC01, are measured for the train running at speeds from 260 km/h to 385 km/h, along two types of track including a slab track and a ballast track and either on the ground surface or in a tunnel. Data analyses are performed for sound pressure overall levels, frequency, area contributions, and possible generation mechanisms, showing how they are affected by train speed, running direction, track type, and tunnel. The results show that, whether TC01 is used as head car or end car, the interior noise characteristics in the VIP cabin are mostly related to aerodynamic noise. Differences in interior noise between tracks become smaller as the train speed increases. The effect of a tunnel on the interior noise is more important for the middle coach than that for the head coach. This study can provide a basis for noise control of high-speed trains.展开更多
The major brassinosteroid （BR） receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 （BRI1） plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization...The major brassinosteroid （BR） receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 （BRI1） plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRII. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFIotl-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFIotl amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bril-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.展开更多
基金Acknowledgments This work was supported by the National Natural Science Foundation of China （30490254, 30671316）, the National Basic Research Program of China （2006CB100102）, and the Hi-Tech Research and Development Program of China （2006AA10Z113, 2006AA10A111）.
文摘MYB-type transcription factors contain the conserved MYB DNA-binding domain of approximately 50 amino acids and are involved in the regulation of many aspects of plant growth, development, metabolism and stress responses. From soybean plants, we identified 156 GmMYB genes using our previously obtained 206 MYB unigenes, and 48 were found to have full-length open-reading frames. Expressions of all these identified genes were examined, and we found that expressions of 43 genes were changed upon treatment with ABA, salt, drought and/or cold stress. Three GmMYB genes, GmMYB76, GmMYB92 and GmMYB177, were chosen for further analysis. Using the yeast assay system, GmMYB76 and GmMYB92 were found to have transactivation activity and can form homodimers. GmMYB177 did not appear to have transactivation activity but can form heterodimers with GmMYB76. Yeast onehybrid assay revealed that all the three GmMYBs could bind to cis-elements TAT AAC GGT TTT TT and CCG GAA AAA AGG AT, but with different affinity, and GmMYB92 could also bind to TCT CAC CTA CC. The transgenic Arabidopsis plants overexpressing GmMYB 76 or GmMYB177 showed better performance than the GmMYB92-transgenic plants in salt and freezing tolerance. However, these transgenic plants exhibited reduced sensitivity to ABA treatment at germination stage in comparison with the wild-type plants. The three GmMYB genes differentially affected a subset of stress-responsive genes in addition to their regulation of a common subset of stress-responsive genes. These resuits indicate that the three GmMYB genes may play differential roles in stress tolerance, possibly through regulation of stress-responsive genes.
基金This work was supported by the National High Tech Projects(2012AA030402 and 2011AA010202)the National Science Foundation of China(61138001,61171024,61171026,61371035 and 11227904)+2 种基金the 111 Project(111-2-05)the Natural Science Foundation of Jiangsu Province(BK2012019)and the 973 Program(2014CB339800).
文摘The terahertz region is a special region of the electromagnetic spectrum that incorporates the advantages of both microwaves and infrared light waves.In the past decade,metamaterials with effective medium parameters or gradient phases have been studied to control terahertz waves and realize functional devices.Here,we present a new approach to manipulate terahertz waves by using coding metasurfaces that are composed of digital coding elements.We propose a general coding unit based on a Minkowski closed-loop particle that is capable of generating 1-bit coding(with two phase states of 0 and 180°),2-bit coding(with four phase states of 0,90°,180°,and 270°),and multi-bit coding elements in the terahertz frequencies by using different geometric scales.We show that multi-bit coding metasurfaces have strong abilities to control terahertz waves by designing-specific coding sequences.As an application,we demonstrate a new scattering strategy of terahertz waves—broadband and wide-angle diffusion—using a 2-bit coding metasurface with a special coding design and verify it by both numerical simulations and experiments.The presented method opens a new route to reducing the scattering of terahertz waves.
基金This work was financially supported by the Natural Science Foundation of China(No.31670181,31670695)the Three New Forestry Engineering Foundation of Jiangsu Province(No.lysx[2016]55).
文摘Ginkgo biloba is grown worldwide as an ornamental plant for its golden leaf color.However,the regulatory mechanism of leaf coloration in G.biloba remains unclear.Here,we compared G.biloba gold-colored mutant leaves and normal green leaves in cytological,physiological and transcriptomic terms.We found that chloroplasts of the mutant were fewer and smaller,and exhibited ruptured thylakoid membranes,indistinct stromal lamellae and irregularly arranged vesicles.Physiological experiments also showed that the mutant had a lower chlorophyll,lower flavonoid and higher carotenoid contents(especially lutein).We further used transcriptomic sequencing to identify 116 differentially expressed genes(DEGs)and 46 transcription factors(TFs)involved in chloroplast development,chlorophyll metabolism,pigment biosynthesis and photosynthesis.Among these,the chlorophyll biosynthesis-related PPO showed down-regulation,while chlorophyll degradation-related NYC/NOL had up-regulated expression in mutant leaves.Z-ISO,ZDS,and LCYE,which are involved in carotenoid biosynthesis were up-regulated.Quantitative real-time PCR(RT-qPCR)further confirmed the altered expression levels of these genes at three stages.The alteration of PPO and NYC/NOL gene expression might affect chlorophyll biosynthesis and promote degradation of chlorophyll b to chlorophyll a,while the up-regulated genes Z-ISO,ZDS and LCYE enhanced carotenoid accumulation.Consequently,changes in the ratio of carotenoids to chlorophylls were the main factors driving the golden leaf coloration in the mutant G.biloba.
文摘Background Photodynamic therapy （PDT） with 5-aminolevulinic acid （ALA） is an emerging technique for the treatment of genital human papillomavirus （HPV）-induced benign and premalignant lesions. We report here in a case series of condyloma acuminata （CA） in pregnancy successfully treated with ALA-PDT. Methods Five pregnant patients with CA received three to four times treatment respectively. Patients were followed up for 6-23 months after treatment. Results The clearance rate of genital warts was 100%. No recurrence was found during the follow-up period. Major adverse events reported were mild erosion, pain, and local edema. All pregnancies resulted in healthy live births without delivery complications. Conclusions PDT with topical ALA seems to be safe and effective in the treatment of CA in pregnancy. It demonstrated high clearance rate of warts, was well-tolerated by patients, and showed no adverse effects on mothers or fetuses. ALA-PDT may be an ideal strategy of treatment for pregnant women with CA.
基金This work is supported by the National Natural Science Foundation of China （91317306）, State Key Basic Research Project （2015CB755702） and the State Key Lab of Plant Genomics.ACKNOWLEDGMENTS No conflict of interest declared.
文摘Ethylene as a gas phytohormone plays significant roles in the whole life cycle of plants, ranging from growth and development to stress responses. A linear ethylene signaling pathway has been established in the dicotyledonous model plant Arabidopsis. However, the ethylene signaling mechanism in monocotyledonous plants such as rice is largely unclear. In this review, We compare the ethylene response phenotypes of dark-grown seedlings of Arabidopsis, rice, and other monocotyledonous plants （maize, wheat, sorghum, and Brachypodium distachyon） and pinpoint that rice has a distinct phenotype of root inhibition but coleoptile promotion in etiolated seedlings upon ethylene treatment. We further summarize the homologous genes of Arabidopsis ethylene signaling components in these monocotyledonous plants and discuss recent progress. Although conserved in most aspects, ethylene signaling in rice has evolved new features compared with that in Arabidopsis. These analyses provide novel insights into the understanding of ethylene signaling in the dicotyledonous Arabidopsis and monocotyledonous plants, particularly rice. Further characterization of rice ethylene-responsive mutants and their corresponding genes will help us better understand the whole picture of ethylene signaling mechanisms in plants.
文摘Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In this study, through whole-genome sequencing of a recombinant inbred line population derived from a cross between a wild soybean ZYD7 and a cultivated soybean HN44, and mapping of quantitative trait loci for seed weight, we discovered that a phosphatase 2C-1 （PP2C-1） allele from wild soybean ZYD7 contributes to the increase in seed weight/size. PP2C-1 may achieve this function by enhancing cell size of integument and activating a subset of seed trait-related genes. We found that PP2C-1 is associ- ated with GmBZR1, a soybean ortholog of Arabidopsis BZR1, one of key transcription factors in brassi- nosteroid （BR） signaling, and facilitate accumulation of dephosphorylated GmBZR1. In contrast, the PP2C-2 allele with variations of a few amino acids at the N-terminus did not exhibit this function. More- over, we showed that GmBZR1 could promote seed weight/size in transgenic plants. Through analysis of cultivated soybean accessions, we found that 40% of the examined accessions do not have the PP2C-1 allele, suggesting that these accessions can be improved by introduction of this allele. Taken together, our study identifies an elite allele PP2C-1, which can enhance seed weight and/or size in soybean, and pinpoints that manipulation of this allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.
基金funded by Muju Tianma Native Local Industrial Center,Korea
文摘Several studies have demonstrated that the Chinese herb Gastrodia elata Blume can protect against amyloid beta-peptide （Ap）-induced cell death. To investigate the possible therapeutic effects of Gastrodia elata Blume on Alzheimer＇s disease, we established a rat model of AIzheimer＇s disease by injecting A325-35 into bilateral hippocampi. These rats were intragastrically administered 500 or 1 000 mg/kg Gastrodia elata Blume per day for 52 consecutive days. Morris water maze tests showed that Gastrodia elata Blume treatment significantly improved the spatial memory of Alzheimer＇s disease rats. Congo red staining revealed that Gastrodia elata Blume significantly reduced the number of amyloid deposits in the hippocampus of these rats. Western blot analysis showed that choline acetyltransferase expression in the medial septum and hippocampus was significantly increased by the treatment of Gastrodia elata Blume, while EIIman method showed significant decrease in the activity of acetylcholinesterase in all three regions （prefrontal cortex, medial septum and hippocampus）. These findings suggest that long-term administration of Gastrodia elata Blume has therapeutic potential for Alzheimer＇s disease.
基金Supported by the National Natural Science Foundation of China under Grant Nos 10827404 and 10774160, the National Basic Research Program of China under Grant Nos 2010CB832805, and the Funds from the Chinese Academy of Sciences.
文摘We demonstrate a precision measurement of local gravity acceleration 9 in Wuhan by a compact cold atom interferometer. The atom interferometer is in vertical Mach-Zehnder configuration realized using a 7r /2 - π - π /2 Raman pulse sequence. Cold atoms were prepared in a magneto-optical trap, launched upward to form an atom fountain, and then coherently manipulated to interfere by stimulated Raman transition. Population signal vs Raman laser phase was recorded as interference fringes, and the local gravity was deduced from the interference signal. We have obtained a resolution of 7 × 10-9g after an integration time of 236s under the best vibrational environment conditions. The absolute g value was derived from the chirp rate with a difference of 1.5 × 10--07 g compared to the gravity reference value. The tidal phenomenon was observed by continuously monitoring the local gravity over 123 h.
基金the National Natural Science Foundation of China，the National Key Basic Research Project，the National Transgenic Research Project
文摘Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. Manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.
基金Supported by National Natural Science Foundation of China,No.81160201 and No.U1303222
文摘AIM: To evaluate the reliability and accuracy of threedimensional(3D) reconstruction for liver resection in patients with hepatic alveolar echinococcosis(HAE).METHODS: One-hundred and six consecutive patients with HAE underwent hepatectomy at our hospital between May 2011 and January 2015. Fifty-nine patients underwent preoperative 3D reconstruction and "virtual" 3D liver resection before surgery(Group A). Another 47 patients used conventional imaging methods for preoperative assessment(Group B). Outcomes of hepatectomy were compared between the two groups.RESULTS: There was no significant difference in preoperative data between the two groups. Compared with patients in Group B, those in Group A had a significantly shorter operation time(227.1 ± 51.4 vs 304.6 ± 88.1 min; P < 0.05), less intraoperative blood loss(308.1 ± 135.4 vs 458.1 ± 175.4 m L; P < 0.05), and lower requirement for intraoperative blood transfusion(186.4 ± 169.6 vs 289.4 ± 199.2 m L; P < 0.05). Estimated resection liver volumes in bothgroups had good correlation with actual graft weight(Group A: r = 0.978; Group B: r = 0.960). There was a significant higher serum level of albumin in Group A(26.3 ± 5.9 vs 22.6 ± 4.3 g/L, P < 0.05). Other postoperative laboratory parameters(serum levels of aminotransferase and bilirubin; prothrombin time) and duration of postoperative hospital stay were similar. Sixteen complications occurred in Group A and 19 in Group B. All patients were followed for 3-46(mean, 17.3) mo. There was no recurrence of lesions in Group A, but two recurrences in Group B. There were three deaths: two from cerebrovascular accident, and one from car accident.CONCLUSION: 3D reconstruction provides comprehensive and precise anatomical information for the liver. It also improves the chance of success and reduces the risk of hepatectomy in HAE.
文摘MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division process remains unknown. Here, we report that an R2R3-type transcription factor gene, AtMYB59, is involved in the regulation of cell cycle progression and root growth. The AtMYB59 protein is localized in the nuclei of onion epidermal cells and has transactivation activity. Expression of AtMYB59 in yeast cells suppresses cell proliferation, and the transfor- mants have more nuclei and higher anenpioid DNA content with longer cells. Mutation in the conserved domain of AtMYB59 abolishes its effects on yeast cell growth. In synchronized Arabidopsis cell suspensions, the AtMYB59 gene is specifically expressed in the S phase during cell cycle progression. Expression and promoter-GUS analysis reveals that the AtMYB59 gene is abundantly expressed in roots. Transgenic plants overexpressing AtMYB59 have shorter roots compared with wild-type plants （Arabidopsis accession Col-0）, and around half of the mitotic cells in root tips are at metaphase. Conversely, the null mutant myb59-1 has longer roots and fewer mitotic cells at metaphase than Col, suggesting that AtMYB59 may inhibit root growth by extending the metaphase of mitotic cells. AtMYB59 regulates many downstream genes, including the CYCB1;1 gene, probably through binding to MYB-responsive elements. These results support a role forAtMYB59 in cell cycle regulation and plant root growth.
文摘目的】建立苹果原料制干适宜性评价模型,实现基于苹果原料指标预测干制品品质的目标,为苹果制干专用化原料的筛选提供方法依据,为明确苹果干制品品质形成的基础物质提供数据支持。【方法】以来自7个不同主产区的21个主栽品种,共34份苹果鲜果样本为研究对象,运用多种数据处理方法建立苹果脆片品质综合评价模型与苹果原料制干适宜性评价模型。(1)利用压差闪蒸干燥方法制备34份苹果鲜果的脆片样本,测定苹果脆片17项品质指标,采用因子分析进行降维并筛选得到苹果脆片品质评价核心指标,运用层次分析法得到脆片核心指标权重值,构建脆片品质综合评价模型并计算得到脆片综合评价得分。(2)测定34份苹果鲜果样本22项品质指标,与脆片核心指标进行相关性分析并筛选出与脆片品质相关的果实特征指标。选用29个样本以果实特征指标为输入,对应脆片综合评价得分为输出,利用误差反向传播(Error Back Propagation,BP)神经网络算法构建学习模型;其余5个样本为验证样本,评价学习模型的预测准确性。变换3组学习样本构建3个学习模型,对比3个模型的预测准确性,验证建模方法的合理性与稳定性。【结果】苹果脆片L*值、脆度、膨化度、可滴定酸含量、可溶性糖含量和粗蛋白含量被确定为不同样本脆片品质综合评价的核心指标,构建的苹果脆片品质综合评价模型为Y_(综合得分)=L*值×0.3724+脆度×0.2665+膨化度×0.1583+可滴定酸含量×0.0890+可溶性糖含量×0.0569+粗蛋白含量×0.0569。34个苹果鲜果样本制得的脆片综合得分范围为0.2069—0.7933,存在较大差异,得分排名前3的苹果样本为‘辽宁华红’‘辽宁华金’和‘山东烟富6号’,排名最后的苹果样本为‘陕西秦冠’。基于脆片核心指标与苹果果实品质指标相关性分析结果,筛选出苹果果实的果形指数、果肉a*值、pH、
基金This work was sponsored by the 973 programs(Nos.2011CBA00200 and 2012CB921803)the National Natural Science Foundation of China(Nos.61490714,11304151 and 61435008)+5 种基金the PhD Programs Foundation of the Ministry of Education of China(No.20120091120020)Yan-Qing Lu appreciates the support from National Science Fund for Distinguished Young Scholars(No.61225026)the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT13021)Biao-Bing Jin appreciates the support from the 973 program(No.2014CB339800)the National Natural Science Foundation of China(Nos.61371035 and 11227904)Cooperative Innovation Centre of Terahertz Science,University of Electronic Science and Technology(Chengdu,China).
文摘Versatile devices,especially tunable ones,for terahertz imaging,sensing and high-speed communication,are in high demand.Liquid crystal based components are perfect candidates in the optical range;however,they encounter significant challenges in the terahertz band,particularly the lack of highly transparent electrodes and the drawbacks induced by a thick cell.Here,a strategy to overcome all these challenges is proposed:Few-layer porous graphene is employed as an electrode with a transmittance of more than 98%.A subwavelength metal wire grid is utilized as an integrated high-efficiency electrode and polarizer.The homogeneous alignment of a high-birefringence liquid crystal is implemented on both frail electrodes via a non-contact photo-alignment technique.A tunable terahertz waveplate is thus obtained.Its polarization evolution is directly demonstrated.Furthermore,quarter-wave plates that are electrically controllable over the entire testing range are achieved by stacking two cells.The proposed solution may pave a simple and bright road toward the development of various liquid crystal terahertz apparatuses.
基金supported by the National Natural Science Foundation of China(Nos.51475390 and U1434201)the National High-Tech R&D Program(863)of China(No.2011AA11A103-2-2)the Program for Changjiang Scholars and Innovative Research Team in University(Nos.IRT1178 and SWJTU12ZT01),China
基金supported by the National Natural Science Foundation of China(Nos.51475390 and U1434201)the National High-Tech R&D Program(863)of China(No.2011AA11A103-4-2)+1 种基金the Fundamental Research Funds for the Central Universitiesthe 2013 Doctoral Innovation Funds of Southwest Jiaotong University,China
基金Project supported by the National Natural Science Foundation of China （Nos. 51475390 and U 1434201）, the National Key Technology R＆D Program of China （Nos. 2016YFB1200506-08 and 2016YFB1200503-02）, and the Scientific Research Foundation of State Key Laboratory of Traction Power （No. 2015TPL_T08）, China
文摘This paper presents an investigation into the characteristics of interior noise of a Chinese high-speed train under several typical conditions. Interior noises within Vehicle TC01, which can be used as a head car or an end car, and Vehicle TP03, the third car counting from TC01, are measured for the train running at speeds from 260 km/h to 385 km/h, along two types of track including a slab track and a ballast track and either on the ground surface or in a tunnel. Data analyses are performed for sound pressure overall levels, frequency, area contributions, and possible generation mechanisms, showing how they are affected by train speed, running direction, track type, and tunnel. The results show that, whether TC01 is used as head car or end car, the interior noise characteristics in the VIP cabin are mostly related to aerodynamic noise. Differences in interior noise between tracks become smaller as the train speed increases. The effect of a tunnel on the interior noise is more important for the middle coach than that for the head coach. This study can provide a basis for noise control of high-speed trains.
文摘The major brassinosteroid （BR） receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 （BRI1） plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRII. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFIotl-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFIotl amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bril-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.
