摘要
为了研究肉桂、山苍子、迷迭香植物精油化学成分及体外抑菌活性,采用滤纸片法测定抑菌圈直径,通过肉汤稀释法和点种法测定其最小抑菌浓度(MIC)、最小杀菌浓度(MBC),并通过气相-质谱法(GC-MS)测定各植物精油的成分及含量。结果显示,肉桂精油浓度在1.118%时就可将5种病原菌全部杀灭;山苍子精油浓度在4.75%时可将S.albus、S.aureus、E.coli、C.albians杀灭,而对P.aeruginosa的杀灭浓度大于9.5%;迷迭香精油在浓度9.5%可将S.albus、S.aureus、C.albicans杀灭,而对E.coli、P.aeruginosa的杀灭浓度大于9.5%。GC-MS结果显示,肉桂精油主要组分为肉桂醛(83.57%)、2′-甲氧基肉桂醛(5.96%),香豆素(2.90%);山苍子精油包括柠檬醛(21.45%)、双戊烯(21.88%)、(Z)-3,7-二甲基-2,6-辛二烯醛(20.96%);迷迭香精油主要含有α-蒎烯(24.09%)、桉树脑(25.81%)、左旋樟脑(25.09%)、异龙脑(9.28%)。结果表明,3种植物精油具有广谱的抑菌活性,能够为开发新型、绿色、安全、高效的抗生素替代品提供理论依据。
In order to study the chemical constituents and antimicrobial activities in vitro of plant essential oils of Cinnamomum cassia,Litsea cubeba and Rosmarinus officinalis,the diameter of antimicrobial circle was measured by filter paper method,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined by broth dilution method and dot seed method,and the components and contents of essential oils were determined by gas chromatography-mass spectrometry.The results displayed that Cinnamomum cassia essential oil could kill all five pathogens at 1.118%concentration;Litsea cubeba essential oil could kill S.albus,S.aureus,E.coli and C.albians at 4.75%concentration,but the killing concentration of P.aeruginosa was more than 9.5%;Rosmarinus officinalis essential oil could kill S.albus,S.aureus and C.albicans at 9.5%concentration,while E.coli and P.aeruginosa at more than 9.5%concentration.The results of GC-MS showed that the main components of Cinnamomum cassia essential oil were cinnamaldehyde(83.57%),2′-methoxycinnamaldehyde(5.96%)and coumarin(2.90%);Litsea cubeba essential oil included citral(21.45%),dipentene(21.88%),(Z):-3,7-dimethyl-2,6-octadienal(20.96%);Rosmarinus officinalis essential oil mainly containedα-pinene(24.09%),eucalyptus(25.81%),levocamphor(25.09%),isoborneol(9.28%).The results showed that three plant essential oils had broad-spectrum antimicrobial activity,which could provide theoretical basis for the development of new,green,safe and efficient alternatives to antibiotics.
作者
牛彪
金川
梁剑平
尚若锋
郝宝成
王学红
杨珍
刘宇
NIU Biao;JIN Chuan;LIANG Jian-ping;SHANG Ruo-feng;HAO Bao-cheng;WANG Xue-hong;YANG Zhen;LIU Yu(Key Laboratory of New Animal Drug Project,Gansu province,Key Laboratory of Veternary Pharmaceutics Discovery,Ministry of Agricultural,Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou,Gansu,730050,China;Bailu Animal Husbandry and Veterinary Station of AnDing District Animal Husbandry and Veterinary Bureau,Dingxi,Gansu,743026,China)
出处
《动物医学进展》
北大核心
2019年第12期18-23,共6页
Progress In Veterinary Medicine
基金
中国农业科学院2018年重大产出科研选题项目(CAAS-ZDXT2018008)
兰州市科技计划项目(2018-1-114)
关键词
植物精油
滤纸片法
最小抑菌浓度
最小杀菌浓度
气相-质谱法
plant essential oil
filter paper method
minimum bacteriostatic concentration
minimum bactericidal concentration
gas chromatography-mass spectrometry
