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A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)
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作者 Melissa L.Starkie Elizabeth V.Fowler +9 位作者 Alexander M.piper Xiaocheng Zhu Pauline Wyatt David Gopurenko Matt N.Krosch Francesca Strutt Karen F.Armstrong Hamish Patrick Mark K.Schutze Mark J.Blacket 《Insect Science》 SCIE CAS CSCD 2024年第4期1285-1295,共11页
Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host ranges.The need to differentiate ... Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host ranges.The need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference colonies.While morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two species.Additionally,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between B.neohumeralis and B.tryoni;therefore,ambiguous samples remain undiagnosed.Here we report the first molecular marker that can consistently distinguish between B.tryoni and B.neohumeralis.Our diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene region.We confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the species.The assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in B.tryoni and the other targeting adjacent SNPs present in B.neohumeralis(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly species.Therefore,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses. 展开更多
关键词 ARMS-PCR COLONY molecular diagnostics PANGOLIN tetra-primer
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Rapid GFAP and Iba1 expression changes in the female rat brain following spinal cord injury 被引量:3
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作者 Mawj Mandwie Jordan A.piper +4 位作者 Catherine A.Gorrie Kevin A.Keay Giuseppe Musumeci Ghaith Al-Badri Alessandro Castorina 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第2期378-385,共8页
Evidence suggests that rapid changes to supporting glia may predispose individuals with spinal cord injury(SCI) to such comorbidities. Here, we interrogated the expression of astrocyte-and microglial-specific markers ... Evidence suggests that rapid changes to supporting glia may predispose individuals with spinal cord injury(SCI) to such comorbidities. Here, we interrogated the expression of astrocyte-and microglial-specific markers glial fibrillary acidic protein(GFAP) and ionized calcium binding adaptor molecule 1(Iba1) in the rat brain in the first 24 hours following SCI. Female Sprague-Dawley rats underwent thoracic laminectomy;half of the rats received a mild contusion injury at the level of the T10 vertebral body(SCI group), the other half did not(Sham group). Twenty-four hours post-surgery the amygdala, periaqueductal grey, prefrontal cortex, hypothalamus, lateral thalamus, hippocampus(dorsal and ventral) in rats were collected. GFAP and Iba1 m RNA and protein levels were measured by real-time quantitative polymerase chain reaction and Western blot. In SCI rats, GFAP m RNA and protein expression increased in the amygdala and hypothalamus. In contrast, gene and protein expression decreased in the thalamus and dorsal hippocampus. Interestingly, Iba1 transcripts and proteins were significantly diminished only in the dorsal and ventral hippocampus, where gene expression diminished. These findings demonstrate that as early as 24 hours post-SCI there are region-specific disruptions of GFAP and Iba1 transcript and protein levels in higher brain regions. All procedures were approved by the University of Technology Sydney Institutional Animal Care and Ethics Committee(UTS ACEC13-0069). 展开更多
关键词 affective disorders ASTROCYTES glial fibrillary acidic protein ionized calcium binding adaptor molecule 1 memory MICROGLIA NEUROTRAUMA spinal cord injury
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Malocclusion May Be Attributed to Variation among 10 Genes 被引量:1
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作者 piper Dizak John Burnheimer +1 位作者 Kathleen Deeley Alexandre R. Vieira 《Open Journal of Stomatology》 2021年第7期263-269,共7页
<strong>Introduction:</strong> The goal of this study was to utilize physical characteristics instead of placing subjects in arbitrary diagnostic categories to test for associations with genetic variants. ... <strong>Introduction:</strong> The goal of this study was to utilize physical characteristics instead of placing subjects in arbitrary diagnostic categories to test for associations with genetic variants. <strong>Methods:</strong> Forty-four single nucleotide polymorphisms were tested for association with specific cephalometric measurements in thirty-nine University of Pittsburgh Dental Registry and DNA Repository orthodontic subjects. Cephalometric measurements included an evaluation of FMA, a Wits appraisal, and a Steiner’s ANB analysis. Genetic markers were genotyped using polymerase chain reaction and Taqman chemistry. Chi-square and Fischer’s exact tests (α = 0.05) were used in investigation of overrepresentation of marker alleles. Samples were divided into groups based upon having an FMA, Wits, or ANB measurement above or below the mean of the cohort studied. Secondary analysis was done for sex and ethnicity to determine their effect on FMA, Wits, or ANB. <strong>Results: </strong>An association between FMA measurements was discovered in the following genes: ACTN3, CASP4, ESR1, FGF13, KRT7, and PITX2. An association between Wits measurements was discovered in the following genes: ACTN2, BTBD11, CASP4, FGF3, and FGF10. No associations were found with ANB.<strong> Conclusions: </strong>Genetic markers in several genes at different loci may contribute to craniofacial deformities in humans. This approach of using physical measurements may be an advantage to placing patients in arbitrary diagnostic categories. 展开更多
关键词 MALOCCLUSION Multifactorial Inheritance Genetic Association Studies Linkage Disequilibrium GENES
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中国与澳大利亚胃溃疡的比较研究 被引量:3
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作者 李增烈 piper,DW 《中华消化杂志》 CAS CSCD 北大核心 1993年第1期61-62,共2页
关键词 中国 澳大利亚 胃溃疡
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Truth lies below: A case report and literature review of typical appearing polyps yet with an atypical diagnosis 被引量:2
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作者 Aaron Fisher Edward Yousif Marc piper 《World Journal of Gastrointestinal Endoscopy》 CAS 2019年第1期54-59,共6页
BACKGROUND Enteropathy associated T-cell lymphoma(EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with cel... BACKGROUND Enteropathy associated T-cell lymphoma(EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with celiac disease, and monomorphic epitheliotropic intestinal T-cell lymphoma(MEITL), formally type 2, which is not associated with celiac disease.CASE SUMMARY We present a 60-year-old African American female, without celiac disease, who presented with abdominal pain, diarrhea, and 30 lb. weight loss over a 3 month period. She was subsequently diagnosed with EATL throughout her entire gastrointestinal tract. She is currently undergoing chemotherapy with EOCH(Etoposide, Oncovin, Cyclophosphamide, and Hydroxydaunorubicin). EATL is most common in the Asian and Hispanic population yet the incidence in African Americans is uncertain and emphasizes the rarity of this case. A literature review was included to further emphasize similarities and differences between our case and previously reported cases of MEITL.CONCLUSION The patient was diagnosed with EATL, immunochemical testing was not conclusive for MEITL however was suggestive of the disease. 展开更多
关键词 ENTEROPATHY associated T-CELL LYMPHOMA Monomorphic epitheliotropic intestinal T-CELL LYMPHOMA Peripheral T-CELL LYMPHOMA Gastrointestinal LYMPHOMA Endoscopy Case report LITERATURE review
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Constitutive AKT Activity Predisposes Lung Fibrosis by Regulating Macrophage, Myofibroblast and Fibrocyte Recruitment and Changes in Autophagy 被引量:2
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作者 Duaa Dakhlallah Yijie Wang +5 位作者 Tierra A. Bobo Emily Ellis Xiaokui Mo Melissa G. piper Timothy D. Eubank Clay B. Marsh 《Advances in Bioscience and Biotechnology》 2019年第10期346-373,共28页
The etiology and pathogenesis of pulmonary fibrosis is poorly understood. We and others reported that M-CSF/CSF-1, M-CSF-R and downstream AKT activation plays an important role in lung fibrosis in mice models and in I... The etiology and pathogenesis of pulmonary fibrosis is poorly understood. We and others reported that M-CSF/CSF-1, M-CSF-R and downstream AKT activation plays an important role in lung fibrosis in mice models and in IPF patients. To understand potential molecular pathways used by M-CSF-R activation to direct lung fibrosis, we used a novel transgenic mouse model that expresses a constitutively-active form of AKT, myristoylated AKT (Myr-Akt), driven by the c-fms (M-CSF-R) promoter. We were particularly interested in the basal immune state of the lungs of these Myr-Akt mice to assess M-CSF-R-related priming for lung fibrosis. In support of a priming effect, macrophages isolated from the lungs of unchallenged Myr-Akt mice displayed an M2-tropism, enhanced co-expression of M-CSF-R and α-SMA, reduced autophagy reflected by reduced expression of the key autophagy genes Beclin-1, MAP1-Lc3a(Lc3a), and MAP1-Lc3b(Lc3b), and increased p62/STSQM1 expression compared with littermate WT mice. Furthermore, Myr-Akt mice had more basal circulating fibrocytes than WT mice. Lastly, upon bleomycin challenge, Myr-Akt mice showed enhanced collagen deposition, increased F4/80+ and CD45+ cells, reduced autophagy genes Beclin-1, Lc3a, and Lc3b expression, and a shorter life-span than WT littermates. These data provide support that M-CSF-R/AKT activation may have a priming effect which can predispose lung tissue to pulmonary fibrosis. 展开更多
关键词 Pulmonary Fibrosis AKT CSF1 M-CSF Receptor MACROPHAGE MYOFIBROBLAST FIBROCYTES AUTOPHAGY
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从北京、上海、广州等大城市的形态改变过程看中国城市形态、结构的转变 被引量:2
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作者 piper Gaubatz 王晓晓 +1 位作者 王楠 林丹丹 《现代城市研究》 2008年第4期27-43,共17页
城市和经济的快速发展正在改变着中国城市基本的自然形态和结构。在城市发展过程中,能折射出国家动态的经济、社会和政策条件的新的模式正逐渐取代了由浓厚的意识形态所形成的带有鉴别性的模式。这篇论文提供了北京、上海和广州等大城... 城市和经济的快速发展正在改变着中国城市基本的自然形态和结构。在城市发展过程中,能折射出国家动态的经济、社会和政策条件的新的模式正逐渐取代了由浓厚的意识形态所形成的带有鉴别性的模式。这篇论文提供了北京、上海和广州等大城市形态转变的模式和过程的分析。对于快速变化的城市,分析主要集中在两点:城市发展中区域的变化;在发展和形式上出现共同的趋势。文章重点谈了土地使用的专业化、交通循环和建筑的高度、规划的过程、城市的更新及房地产市场的私有化等问题。 展开更多
关键词 城市形态 结构转型 城市规划 城市改造
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从北京,上海,广州等大城市形态转变的模式和过程中看中国的城市转变 被引量:2
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作者 piper Gaubatz 王晓晓 +1 位作者 王楠 林丹丹 《人文地理》 CSSCI 北大核心 2009年第4期77-80,共4页
新的城市和经济的发展正在改变着中国城市基本的自然形态和结构。在城市发展的过程当中,能折射出国家动态的经济,社会和政策条件的新的模式正逐渐取代了由浓厚的意识形态所形成的带有鉴别性的模式。论文提供了北京,上海和广州等大城市... 新的城市和经济的发展正在改变着中国城市基本的自然形态和结构。在城市发展的过程当中,能折射出国家动态的经济,社会和政策条件的新的模式正逐渐取代了由浓厚的意识形态所形成的带有鉴别性的模式。论文提供了北京,上海和广州等大城市形态转变的模式和过程的分析。重点谈到了土地使用的专业化,交通循环和建筑的高度,规划的过程,城市的更新及房地产市场的私有化等问题。20世纪见证了,中国大城市外形和构造的戏剧性改变。中国城市国际化的趋势日趋明朗化。中国文化的独特性以及中国社会主义制度的独特性,表明了中国城市的将来仍然与众不同。 展开更多
关键词 城市模式 交通循环 城市规划 城市 改造
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Brain TXA_2 and PGI_2 levels after head injury with secondary insults 被引量:1
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作者 费舟 章翔 +3 位作者 宋少军 I R piper D Thomson J D Miller 《Chinese Journal of Traumatology》 CAS 1998年第1期49-52,共4页
Objective The brain TXA_2 and PGl_2 levelsin a new rodent model of impact accelerationdiffuse brain injury alone and with hypotentionand hyperthermia in combination were ob-served to look into the relationship between... Objective The brain TXA_2 and PGl_2 levelsin a new rodent model of impact accelerationdiffuse brain injury alone and with hypotentionand hyperthermia in combination were ob-served to look into the relationship betweenTXA_2,PGI_2 levels and different types of headinjury. Methods Thirty-two SD rats were ran- 展开更多
关键词 头部损伤 TXA2 PGI2
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Learning from Taiyuan: Chinese cities as urban sustainability laboratories 被引量:1
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作者 piper Gaubatz Dean Hanink 《Geography and Sustainability》 2020年第2期118-126,共9页
China and the United States are the two most significant nations in the contemporary global food and agricultural network.In addition,they are two of the most important innovators with respect to the development of ne... China and the United States are the two most significant nations in the contemporary global food and agricultural network.In addition,they are two of the most important innovators with respect to the development of new crop varieties,agro-technologies,farm products,markets and consumer issues,such as consumer resistance to genetically modified foods,among others.In the face of an ever-complex web of interactions,technologies and products among producers and consumers in both nations,there are far more structural similarities than differences in the food and agriculture sectors of these two nations.This essay,adopting some of the themes of the Sino-American Symposium on Future Issues Affecting Quality of Life,presents a limited but representative comparative assessment of three of the most important shared challenges impacting the agricultural sectors of China and the United States for the period from 2000 to the present including 1)environmental challenges related to agricultural water supply,2)declines in farm labor and rural population,and 3)growing food-related concerns and challenges.For both nations,excessive and unsustainable groundwater consumption has lowered water tables and limited crop production.Rural populations and farm workforces in both nations are also declining,leading to labor challenges in both nations.Finally,concerns regarding food safety are also very similar with major challenges to the farm sector associated with consumer resistance to genetically modified food crops and sanitation issues linked to lengthening supply chains.All of these issues threaten the development of sustainable agricultural production systems. 展开更多
关键词 AGRICULTURE Food safety China United States Sino-US comparative study
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Er:Yb:YCOB晶体制备及其激光二极管抽运激光特性 被引量:1
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作者 葛炳辉 张怀金 +6 位作者 徐现刚 王继扬 蒋民华 Phillip Burns Judith M.Dawes PU Wang James A.piper 《人工晶体学报》 EI CAS CSCD 北大核心 2003年第5期420-422,共3页
研究了Er:Yb:YCa_4O(BO_3)_3(简称 Er:Yb:YCOB)的多晶制备和单晶生长,用提拉法生长出光学质量优良的Er:Yb:YCCOB单晶,测量了其吸收光谱和荧光光谱,分析了其能级和泵浦原理,并进行了以激光二极管为抽运源的激光试验,实现了Er:Yb:YCOB晶... 研究了Er:Yb:YCa_4O(BO_3)_3(简称 Er:Yb:YCOB)的多晶制备和单晶生长,用提拉法生长出光学质量优良的Er:Yb:YCCOB单晶,测量了其吸收光谱和荧光光谱,分析了其能级和泵浦原理,并进行了以激光二极管为抽运源的激光试验,实现了Er:Yb:YCOB晶体的在1.55μm附近110mW的激光输出,且斜效率达18.9%。 展开更多
关键词 Er:Yb:YCa4O(BO3)3 晶体生长 制备 激光二极管 激光特性 多晶 单晶
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Dynamic changes in proprotein convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation
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作者 Shuqin Zhan An Zhou +1 位作者 Chelsea piper Tao Yang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第1期83-89,共7页
In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cul... In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling-positive neurons significantly increased and proprotein convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that proprotein convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury. 展开更多
关键词 neural regeneration brain injury proprotein convertase 2 cortex neuron cerebralischemia/reperfusion oxygen-glucose deprivation in vivo study in vitro study grants-supportedpaper photographs-containing paper NEUROREGENERATION
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投资与合作
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作者 DLA piper Rocky T. Lee Matr Adler Chris Terry 《投资与合作》 2007年第5期95-98,共4页
关键词 投资 合作 金融 经济
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内皮的存在掩盖了邻苯三酚与亚甲蓝直接舒张大鼠肠系膜动脉的效应(英文)
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作者 李元建 Sue piper DUCKLES 《中国药理学报》 CSCD 1994年第1期21-27,共7页
在灌注的大鼠肠系膜血管,邻苯三酚与亚甲蓝能增强甲氧胺或电刺激释放去甲肾上腺素的缩血管效应。然而,当亚硝基精氨酸甲酯存在或去内皮时,两者可抑制TNS所致的缩血管效应,去除内皮后,邻苯三酚与亚甲蓝产生浓度依赖性舒血管效应,结果提示... 在灌注的大鼠肠系膜血管,邻苯三酚与亚甲蓝能增强甲氧胺或电刺激释放去甲肾上腺素的缩血管效应。然而,当亚硝基精氨酸甲酯存在或去内皮时,两者可抑制TNS所致的缩血管效应,去除内皮后,邻苯三酚与亚甲蓝产生浓度依赖性舒血管效应,结果提示,血管内皮存在可能掩盖邻苯三酚与亚甲蓝两者的直接舒张血管平滑肌作用。 展开更多
关键词 肠系膜动脉 邻苯三酚 药理学
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TAVI:经股动脉径路的指征和结果
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作者 D. Horstkotte W. Scholtz +3 位作者 C. piper J. Borgermann J. Gummert M. Wiemer 《中国心血管病研究》 CAS 2010年第8期634-635,共2页
关键词 经导管主动脉瓣置换 主动脉瓣置换 并发症
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胎儿期肠扩张对腹裂新生儿临床预后的影响
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作者 piper H.G. Jaksic T. 刘凯 《世界核心医学期刊文摘(儿科学分册)》 2006年第A10期38-39,共2页
Purpose: In fetuses with gastroschisis, the importance of ultrasonographic bowel dilation remains controversial. The outcome of patients with gastroschisis with and without prenatal bowel dilation is reported. Methods... Purpose: In fetuses with gastroschisis, the importance of ultrasonographic bowel dilation remains controversial. The outcome of patients with gastroschisis with and without prenatal bowel dilation is reported. Methods: From 2000 to 2004, 27 neonates with gastroschisis were followed at a single center. Thirteen patients had prenatal ultrasonographic bowel dilation (diameter, ≥ 6 mm; range, 6- 35 mm). Outcomes of those with and without dilation were compared using 2 sample t tests and logistic regression. Results: Time to initiation of enteral nutrition varied significantly between groups (20.4 ± 11.7 days vs 12.5 ± 4.3 days, P < 0.05). A trend toward a reduced rate of primary closure was seen in those with dilation (23% vs 50% , P = 0.06). No significant difference was found when considering mortality, gestational age, time in the intensive care unit (ICU), time on parenteral nutrition, or length of stay. Prenatal bowel dilation, a longer ICU stay, and later gestational age independently predicted readmission for bowel obstruction (P < 0.001). Conclusion: Infants with gastroschisis and prenatal bowel dilation were significantly slower to initiate enteral feeding and tended to have a reduced incidence of primary closure. This did not translate into increased mortality, time on parenteral nutrition, time in the ICU, or length of stay. However, dilation was associated with readmission for bowel obstruction. 展开更多
关键词 胎儿期 腹裂 胃肠道外营养 扩张者 超声诊断 超声检查 前肠 愈合率 胃肠外营养 肠道营养
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适用于全双工蜂窝手机的GPS低噪声放大器
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作者 Ian piper 《中国集成电路》 2006年第9期43-47,共5页
关键词 CDMA手机 GPS数据 低噪声放大器 全双工蜂窝手机 低噪放大器 WCDMA 高科技公司 同步采集
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Examination of Sexually Dimorphic Behavior on the Novel-Image Novel-Location Recognition Memory Test
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作者 Brian J. piper Alia L. Yasen Jeremy K. Miller 《Journal of Behavioral and Brain Science》 2011年第3期134-139,共6页
Objectives: Sex differences in object location memory favoring females appear to be a replicable phenomenon but may also depend on the task demands. This investigation evaluated if females outperformed males at both a... Objectives: Sex differences in object location memory favoring females appear to be a replicable phenomenon but may also depend on the task demands. This investigation evaluated if females outperformed males at both a short (immediate) and long (half-hour) interval between the learn and test condition using a recently developed version of the Novel-Image Novel-Location (NINL) test (Piper et al. 2011, Physiology & Behavior, 103, 513 - 522). Methods: Young-adults (N = 184) completed a standardized handedness inventory and the NINL. Results: Participants assigned to the Immediate and Delayed conditions did not differ in age, sex, or handedness. The NINL total score was higher among females at the Immediate, but not Delayed, interval. However, within the Delayed condition, females excelled at correctly identifying the unchanged items with a similar pattern for the Novel-Location (NL) scale. Conclusion: These findings are consistent with the view that sexually dimorphic performance favoring females in neurocognitive function can also extend to tasks that have a spatial component. 展开更多
关键词 APOLIPOPROTEIN E FEMALE Learning MEMORY
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用于802.11a/HiperLAN和HiSWANa接收机的两级低噪声放大器设计
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作者 Ian piper Al Ward 《世界电子元器件》 2004年第10期59-61,共3页
本文分析了一个小型(6 mm×15 mm)两极、低噪声、无限稳定的放大器设计,用于802.11a、HiperLAN2和HiSWANa接收机应用.在5 5GHz时,放大器具有222dB增益、1.4dB噪声系数、线性输出功率(P-1dB)+11.5dBm、三阶输出截距点(OIP3)+28dBm,... 本文分析了一个小型(6 mm×15 mm)两极、低噪声、无限稳定的放大器设计,用于802.11a、HiperLAN2和HiSWANa接收机应用.在5 5GHz时,放大器具有222dB增益、1.4dB噪声系数、线性输出功率(P-1dB)+11.5dBm、三阶输出截距点(OIP3)+28dBm,频率覆盖目前北美、欧洲和日本无线局域网络规定使用的5 GHz频谱部分. 展开更多
关键词 接收机 低噪声放大器 802.11a 两级 噪声系数 无线局域网络 HIPERLAN2 设计 输出 小型
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The role of Src family kinases in mediating M-CSF receptor signaling and monocytic cell survival
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作者 Yijie Wang Melissa G. piper Clay B. Marsh 《Advances in Bioscience and Biotechnology》 2012年第5期592-602,共11页
Previously, we reported that M-CSF induced monocyte survival through the activation of Akt, p38MAPK and Erk1/2 kinases. Here, we found that Src family kinases were upstream of these kinases and played a central role i... Previously, we reported that M-CSF induced monocyte survival through the activation of Akt, p38MAPK and Erk1/2 kinases. Here, we found that Src family kinases were upstream of these kinases and played a central role in regulating M-CSF-induced monocyte survival. We observed that M-CSF promoted c-Src activation in monocytes and MDMs in a time-dependent manner. Src inhibitors reduced M-CSF-mediated phosphorylation of the M-CSF receptor (M-CSFR), Akt, Erk1/2, and p38 MAPK. We also observed that Src directly phosphorylated the M-CSFR. Notably, the inhibitors blocked phosphorylation of specific tyrosine residues within the M-CSFR. We further demonstrated that the Src inhibitor, PP2, attenuated M-CSF-induced NF-κB activation and M-CSF-induced monocyte survival. These findings indicated that Src family kinases mediate monocyte survival through the regulation of receptor phosphorylation and modulation of downstream signaling events. Thus, we predict that targeting Src family kinases may have therapeutic implication in inflammatory diseases. 展开更多
关键词 MONOCYTE/MACROPHAGE M-CSF RECEPTOR SRC Family KINASES Cell SURVIVAL
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