为了解湖南省猪源粪肠球菌临床分离株的耐药性及各耐药基因的分布情况,使用K-B法检测了42株猪源粪肠球菌对11种抗生素的敏感性,采用PCR方法检测了粪肠球菌中8种耐药基因的分布情况。结果显示,湖南省临床分离的42株粪肠球菌对大部分的抗...为了解湖南省猪源粪肠球菌临床分离株的耐药性及各耐药基因的分布情况,使用K-B法检测了42株猪源粪肠球菌对11种抗生素的敏感性,采用PCR方法检测了粪肠球菌中8种耐药基因的分布情况。结果显示,湖南省临床分离的42株粪肠球菌对大部分的抗生素高度耐药,对四环素、氯霉素、苯唑青霉素、红霉素、米诺霉素、左氧氟沙星、高浓度庆大霉、高浓度链霉素、环丙沙星、万古霉素、青霉素G的耐药率依次为100%、92.9%、88.1%、83.3%、81%、57.1%、52.4%、47.6%、45.2%、31%、11.9%;耐药基因的检出率为Aac(6)'/aph(2'')97.6%、ant(6)'-Ⅰ90%、aph(3)'-Ⅲ76.2%、tet M 90.5%、erm B 73.8%、Van A 4.8%、Van B 54.8%、Van C 88.1%。从表型与基因型共同分析粪肠球菌的耐药性,发现粪肠球菌的多耐药性严重,其耐药表型与耐药基因并不完全一致。展开更多
The geochemical properties of sediment in the nearby seawater areas of Hailing Bay of the western Guangdong province were first time investigated in the dry period of 2001 and the flood period of 2002, respectively. T...The geochemical properties of sediment in the nearby seawater areas of Hailing Bay of the western Guangdong province were first time investigated in the dry period of 2001 and the flood period of 2002, respectively. The temporal and spatial characteristics of Cu, Pb, Zn, Cd and Hg concentrations, organic carbon and total sulfides contents, and granulometric distribution in the surface sediment from the Hailing Bay were analyzed. Results demonstrated that there were no remarkable changes in the composition of sediment between the dry period and the flood period; the average concentrations of Cu, Pb, Zn, Cd and Hg were 32.1 ± 17.5, 36.1 ± 10.0, 107.0 ± 37.4, 0.26± 0.15 and 0.109 ± 0.073 mg·kg-1, respectively; the organic carbon and total sulfides contents were 1.33 ± 0.53 % and 140.13 ± 136.42 mg·kg-1, respectively; surface sediments were dominated by clayey silt and sand, and their mean grain size was 4.62 ± 2.36 φ and in the range 0.52-7.55 φ; there existed obvious correlation between the concentrations of Cu, Pb, Zn and Hg, and among Cu, Pb, Zn and Hg concentrations, organic carbon contents and grain size of sediments.展开更多
文摘为了解湖南省猪源粪肠球菌临床分离株的耐药性及各耐药基因的分布情况,使用K-B法检测了42株猪源粪肠球菌对11种抗生素的敏感性,采用PCR方法检测了粪肠球菌中8种耐药基因的分布情况。结果显示,湖南省临床分离的42株粪肠球菌对大部分的抗生素高度耐药,对四环素、氯霉素、苯唑青霉素、红霉素、米诺霉素、左氧氟沙星、高浓度庆大霉、高浓度链霉素、环丙沙星、万古霉素、青霉素G的耐药率依次为100%、92.9%、88.1%、83.3%、81%、57.1%、52.4%、47.6%、45.2%、31%、11.9%;耐药基因的检出率为Aac(6)'/aph(2'')97.6%、ant(6)'-Ⅰ90%、aph(3)'-Ⅲ76.2%、tet M 90.5%、erm B 73.8%、Van A 4.8%、Van B 54.8%、Van C 88.1%。从表型与基因型共同分析粪肠球菌的耐药性,发现粪肠球菌的多耐药性严重,其耐药表型与耐药基因并不完全一致。
文摘The geochemical properties of sediment in the nearby seawater areas of Hailing Bay of the western Guangdong province were first time investigated in the dry period of 2001 and the flood period of 2002, respectively. The temporal and spatial characteristics of Cu, Pb, Zn, Cd and Hg concentrations, organic carbon and total sulfides contents, and granulometric distribution in the surface sediment from the Hailing Bay were analyzed. Results demonstrated that there were no remarkable changes in the composition of sediment between the dry period and the flood period; the average concentrations of Cu, Pb, Zn, Cd and Hg were 32.1 ± 17.5, 36.1 ± 10.0, 107.0 ± 37.4, 0.26± 0.15 and 0.109 ± 0.073 mg·kg-1, respectively; the organic carbon and total sulfides contents were 1.33 ± 0.53 % and 140.13 ± 136.42 mg·kg-1, respectively; surface sediments were dominated by clayey silt and sand, and their mean grain size was 4.62 ± 2.36 φ and in the range 0.52-7.55 φ; there existed obvious correlation between the concentrations of Cu, Pb, Zn and Hg, and among Cu, Pb, Zn and Hg concentrations, organic carbon contents and grain size of sediments.
