The recently discovered native endomorphin 2 plays an important role in opioid analgesia, but its metabolic fate in the organism remains relatively little known. The successful synthesis of endomorphin 2 was carried o...The recently discovered native endomorphin 2 plays an important role in opioid analgesia, but its metabolic fate in the organism remains relatively little known. The successful synthesis of endomorphin 2 was carried out by solution method. The C teminal dipeptide and the N terminal dipeptide have been synthesized, respectively, and then coupled successfully. High performance liquid chromatography and electrospray ionization mass spectrometry were used to identify the degradation products from the incubation of endomorphin 2 with brain synaptic membrane. The patterns of peptide metabolites indicated that brain synaptic membrane associated peptidases hydrolyzed first at the N terminal, and then split the peptide at the Phe Phe bond.展开更多
In order to explain the special antibiotic activity of urine, various chromatographic techniques were applied to separate and purify the key component from the healthy male urine. The structure of purified component w...In order to explain the special antibiotic activity of urine, various chromatographic techniques were applied to separate and purify the key component from the healthy male urine. The structure of purified component was identified by mass spectrometry and NMR. The results indicated that the substance separated from the male urine was trimethoprim. This is the first report of trimethoprim separated from urine.展开更多
Gly\| L His L Lys(GHK) was synthesized by liquid\|phase method. The HPLC analysis indicated that the proteolysis of GHK was initiated at the N terminal in liver homogenate. The result suggested that GHK was rapidly de...Gly\| L His L Lys(GHK) was synthesized by liquid\|phase method. The HPLC analysis indicated that the proteolysis of GHK was initiated at the N terminal in liver homogenate. The result suggested that GHK was rapidly degraded by aminopeptidases in liver.展开更多
文摘The recently discovered native endomorphin 2 plays an important role in opioid analgesia, but its metabolic fate in the organism remains relatively little known. The successful synthesis of endomorphin 2 was carried out by solution method. The C teminal dipeptide and the N terminal dipeptide have been synthesized, respectively, and then coupled successfully. High performance liquid chromatography and electrospray ionization mass spectrometry were used to identify the degradation products from the incubation of endomorphin 2 with brain synaptic membrane. The patterns of peptide metabolites indicated that brain synaptic membrane associated peptidases hydrolyzed first at the N terminal, and then split the peptide at the Phe Phe bond.
文摘In order to explain the special antibiotic activity of urine, various chromatographic techniques were applied to separate and purify the key component from the healthy male urine. The structure of purified component was identified by mass spectrometry and NMR. The results indicated that the substance separated from the male urine was trimethoprim. This is the first report of trimethoprim separated from urine.
文摘Gly\| L His L Lys(GHK) was synthesized by liquid\|phase method. The HPLC analysis indicated that the proteolysis of GHK was initiated at the N terminal in liver homogenate. The result suggested that GHK was rapidly degraded by aminopeptidases in liver.
