目的通过检测血清巨噬细胞移动抑制因子(MIF),探讨冠心病患者不同类型心绞痛及冠脉病变程度与MIF的相关性。方法根据冠脉造影结果,将115例住院患者分为对照组53例和冠心病组62例。冠心病组按Braunwald分类方法,根据临床表现分为稳定型...目的通过检测血清巨噬细胞移动抑制因子(MIF),探讨冠心病患者不同类型心绞痛及冠脉病变程度与MIF的相关性。方法根据冠脉造影结果,将115例住院患者分为对照组53例和冠心病组62例。冠心病组按Braunwald分类方法,根据临床表现分为稳定型心绞痛组(SAP)10例和不稳定型心绞痛组(UAP)52例,根据Gensini积分法对冠心病患者冠状动脉狭窄程度进行定量评估,采用酶联免疫吸附试验(ELISA)检测两组血清MIF浓度。结果冠心病组(65.94μg/L)MIF水平明显高于对照组(48.08μg/L)。在冠心病组中,UAP组血清MIF浓度明显高于SAP组,同时SAP组和UAP组血清MIF浓度均高于对照组(65.94μg/L vs 48.08μg/L,P<0.05)。不同冠脉病变程度之间血清MIF浓度无统计学差异。结论血清MIF浓度的增高可能与冠心病的发生发展相关,同时MIF水平的高低可以反映冠脉粥样斑块的稳定性,为冠心病的诊断及干预治疗提供依据,但冠脉狭窄程度与血清MIF浓度无明显相关性,不能用于预测冠脉病变情况。展开更多
目的探讨烟雾主要成分苯并芘(Ba P)对RAW264.7单核-吞噬细胞中基质金属蛋白酶(MMPs)的影响,为研究吸烟与腹主动脉瘤的关联提供相关依据。方法应用免疫印迹法观察Ba P对体外培养的RAW264.7单核-吞噬细胞基质金属蛋白酶-2/9/12(MMP-2/9/12...目的探讨烟雾主要成分苯并芘(Ba P)对RAW264.7单核-吞噬细胞中基质金属蛋白酶(MMPs)的影响,为研究吸烟与腹主动脉瘤的关联提供相关依据。方法应用免疫印迹法观察Ba P对体外培养的RAW264.7单核-吞噬细胞基质金属蛋白酶-2/9/12(MMP-2/9/12)的影响,并验证其是否通过NF-κB信号通路。结果 Ba P暴露使培养巨噬细胞NF-κB活化,同时伴随MMP-9、MMP-12表达升高,而PDTC,核转录因子NF-κB的抑制剂,可抑制NF-κB活化,同时伴随MMP-9、MMP-12表达下降。结论 Ba P可通过NF-κB通路诱导RAW264.7单核-吞噬细胞MMP-9、MMP-12表达升高。展开更多
A MEMS piezoresistive magnetic field sensor based on a silicon bridge structure has been simulated and tested. The sensor consists of a silicon sensitivity diaphragm embedded with a piezoresistive Wheatstone bridge, a...A MEMS piezoresistive magnetic field sensor based on a silicon bridge structure has been simulated and tested. The sensor consists of a silicon sensitivity diaphragm embedded with a piezoresistive Wheatstone bridge, and a ferromagnetic magnet adhered to the sensitivity diaphragm. When the sensor is subjected to an external magnetic field, the magnetic force bends the silicon sensitivity diaphragm, producing stress and resistors change of the Wheatstone bridge and the output voltage of the sensor. Good agreement is observed between the theory and measurement behavior of the magnetic field sensor. Experimental results demonstrate that the maximum sensitivity and minimum resolution are 48 mV/T and 160 μT, respectively, making this device suitable for strong magnetic field measurement. Research results indicate that the sensor repeatability and dynamic response time are about 0.66% and 150 ms, respectively.展开更多
文摘目的通过检测血清巨噬细胞移动抑制因子(MIF),探讨冠心病患者不同类型心绞痛及冠脉病变程度与MIF的相关性。方法根据冠脉造影结果,将115例住院患者分为对照组53例和冠心病组62例。冠心病组按Braunwald分类方法,根据临床表现分为稳定型心绞痛组(SAP)10例和不稳定型心绞痛组(UAP)52例,根据Gensini积分法对冠心病患者冠状动脉狭窄程度进行定量评估,采用酶联免疫吸附试验(ELISA)检测两组血清MIF浓度。结果冠心病组(65.94μg/L)MIF水平明显高于对照组(48.08μg/L)。在冠心病组中,UAP组血清MIF浓度明显高于SAP组,同时SAP组和UAP组血清MIF浓度均高于对照组(65.94μg/L vs 48.08μg/L,P<0.05)。不同冠脉病变程度之间血清MIF浓度无统计学差异。结论血清MIF浓度的增高可能与冠心病的发生发展相关,同时MIF水平的高低可以反映冠脉粥样斑块的稳定性,为冠心病的诊断及干预治疗提供依据,但冠脉狭窄程度与血清MIF浓度无明显相关性,不能用于预测冠脉病变情况。
文摘目的探讨烟雾主要成分苯并芘(Ba P)对RAW264.7单核-吞噬细胞中基质金属蛋白酶(MMPs)的影响,为研究吸烟与腹主动脉瘤的关联提供相关依据。方法应用免疫印迹法观察Ba P对体外培养的RAW264.7单核-吞噬细胞基质金属蛋白酶-2/9/12(MMP-2/9/12)的影响,并验证其是否通过NF-κB信号通路。结果 Ba P暴露使培养巨噬细胞NF-κB活化,同时伴随MMP-9、MMP-12表达升高,而PDTC,核转录因子NF-κB的抑制剂,可抑制NF-κB活化,同时伴随MMP-9、MMP-12表达下降。结论 Ba P可通过NF-κB通路诱导RAW264.7单核-吞噬细胞MMP-9、MMP-12表达升高。
基金Project supported by the National Natural Science Foundation of China(No.60871024)
文摘A MEMS piezoresistive magnetic field sensor based on a silicon bridge structure has been simulated and tested. The sensor consists of a silicon sensitivity diaphragm embedded with a piezoresistive Wheatstone bridge, and a ferromagnetic magnet adhered to the sensitivity diaphragm. When the sensor is subjected to an external magnetic field, the magnetic force bends the silicon sensitivity diaphragm, producing stress and resistors change of the Wheatstone bridge and the output voltage of the sensor. Good agreement is observed between the theory and measurement behavior of the magnetic field sensor. Experimental results demonstrate that the maximum sensitivity and minimum resolution are 48 mV/T and 160 μT, respectively, making this device suitable for strong magnetic field measurement. Research results indicate that the sensor repeatability and dynamic response time are about 0.66% and 150 ms, respectively.
