REV3 encodes a catalytic subunit of DNA polymerase ; required for translesion DNA synthesis. The inhibition of REV3 expression induces persistent DNA damage and growth arrest in the G1 phase, which is initiated by p53...REV3 encodes a catalytic subunit of DNA polymerase ; required for translesion DNA synthesis. The inhibition of REV3 expression induces persistent DNA damage and growth arrest in the G1 phase, which is initiated by p53 activation. We speculated thatp53 plays a critical role in regulating apoptosis and cell growth through inhibition of REV3. In this study, we found that experimental suppression of REV3 induced apoptosis and arrested colon cancer at the G1 phase. Surprisingly, suppression of p53 promoted REV3 expression and the accumulation of S-phase cells, suggesting that excessive REV3 activity interferes with replicative DNA synthesis. The above observations collectively reveal genetic interactions between REV3 andp53 in the regulation of apoptosis and cell growth in colon cancer cells.展开更多
基金supported by the National Natural Science Foundation of China(81060170,31360251)the Ministry of Education‘‘Chunhui Research Grant’’(Z2011056)
文摘REV3 encodes a catalytic subunit of DNA polymerase ; required for translesion DNA synthesis. The inhibition of REV3 expression induces persistent DNA damage and growth arrest in the G1 phase, which is initiated by p53 activation. We speculated thatp53 plays a critical role in regulating apoptosis and cell growth through inhibition of REV3. In this study, we found that experimental suppression of REV3 induced apoptosis and arrested colon cancer at the G1 phase. Surprisingly, suppression of p53 promoted REV3 expression and the accumulation of S-phase cells, suggesting that excessive REV3 activity interferes with replicative DNA synthesis. The above observations collectively reveal genetic interactions between REV3 andp53 in the regulation of apoptosis and cell growth in colon cancer cells.
