The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a prote...The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.展开更多
为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后...为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后3个区段的组织蛋白进行了比较分析.结果表明:中肠前、中、后3个区段组织蛋白的点数、位置、点形等差异较小.在中肠前部和后部组织中找出的4个差异蛋白点,分别为脂蛋白前体、囊膜氢离子泵ATP合酶B亚基、α胰蛋白酶A链和肌动蛋白;在中肠中部找出的一个差异蛋白点为Kun itz型胰蛋白酶抑制剂A链.这些蛋白点可能在BmDNV-ZJ侵染感受性家蚕中肠组织细胞中起一定的作用.展开更多
文摘The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.
文摘为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后3个区段的组织蛋白进行了比较分析.结果表明:中肠前、中、后3个区段组织蛋白的点数、位置、点形等差异较小.在中肠前部和后部组织中找出的4个差异蛋白点,分别为脂蛋白前体、囊膜氢离子泵ATP合酶B亚基、α胰蛋白酶A链和肌动蛋白;在中肠中部找出的一个差异蛋白点为Kun itz型胰蛋白酶抑制剂A链.这些蛋白点可能在BmDNV-ZJ侵染感受性家蚕中肠组织细胞中起一定的作用.
