目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnV...目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnVNS)组、耳穴-迷走神经刺激(taVNS)组,每组9只。正常组予普通饲料喂养,模型组、tnVNS组和taVNS组予高糖高脂饲料喂养联合腹腔注射小剂量链尿佐菌素以制备IGT模型。造模后对tnVNS组和taVNS组大鼠连续干预4周,tnVNS组刺激部位为耳缘区(无迷走神经分布)、taVNS组刺激部位为耳甲区(有迷走神经分布),强度为2 mA,频率为疏密波2 Hz/15 Hz,每次30 min,每天1次,共4周。整个实验持续10周,记录各组大鼠的体质量、空腹血糖(FPG)、餐后2 h血糖(2 h PG),ELISA法,检测血浆胰岛素(INS)、胰高血糖素(GC)、糖化血红蛋白(GHbA1c)水平,Western blot法检测INR在下丘脑、肝脏、骨骼肌中的表达量。结果:造模后与正常组比较,其余3组大鼠体质量均明显增加(P<0.001),FPG和2 h PG升高(P<0.05,P<0.001),符合IGT的诊断标准,提示造模成功。干预后与正常组比较,模型组大鼠体质量明显增加(P<0.001),FPG、2 h PG及GC、GHbA1c含量明显升高(P<0.05,P<0.01),血浆INS含量及下丘脑、肝脏、骨骼肌INR表达明显降低(P<0.001,P<0.01,P<0.05)。干预后与模型组比较,taVNS组大鼠体质量、FPG、2 h PG、血浆GC含量明显降低(P<0.01,P<0.05),INS含量及下丘脑、肝脏、骨骼肌中INR表达明显升高(P<0.001,P<0.01,P<0.05)。干预后与tnVNS组比较,taVNS组大鼠FPG和2 h PG值降低(P<0.05),INS含量及下丘脑、肝脏中INR表达明显升高(P<0.001,P<0.05,P<0.01)。结论:经皮耳穴-迷走神经刺激可调节IGT模型大鼠的FPG和2 h PG,降低GC,提高INS敏感性,上调下丘脑、肝脏、骨骼肌中INR的表达。展开更多
Treatment by auricular acupuncture has a long history. Ear-acupoint research has been advancing step by step in China and also in Europe. Auricles are rich in nerves, therefore a close relationship with different func...Treatment by auricular acupuncture has a long history. Ear-acupoint research has been advancing step by step in China and also in Europe. Auricles are rich in nerves, therefore a close relationship with different functions of the human body has been proved by the research teams of the two main authors of this article from China and Austria. In recent years, great progress has been made in the research of regulating human body functions through electroacupuncture at the auricular branch of the vagus nerve, which is part of auricular acupuncture therapy. It is well known that the auricular branch of the vagus nerve is the only peripheral pathway to the cerebral cortex. Studies of the Chinese team on hypertension, diabetes, epilepsy and depression have shown that the mechanism of auricular vagus nerve stimulation （VNS） may be comparable with cervical VNS in terms of pathways. Auricular VNS has a broad clinical application prospect.展开更多
文摘目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnVNS)组、耳穴-迷走神经刺激(taVNS)组,每组9只。正常组予普通饲料喂养,模型组、tnVNS组和taVNS组予高糖高脂饲料喂养联合腹腔注射小剂量链尿佐菌素以制备IGT模型。造模后对tnVNS组和taVNS组大鼠连续干预4周,tnVNS组刺激部位为耳缘区(无迷走神经分布)、taVNS组刺激部位为耳甲区(有迷走神经分布),强度为2 mA,频率为疏密波2 Hz/15 Hz,每次30 min,每天1次,共4周。整个实验持续10周,记录各组大鼠的体质量、空腹血糖(FPG)、餐后2 h血糖(2 h PG),ELISA法,检测血浆胰岛素(INS)、胰高血糖素(GC)、糖化血红蛋白(GHbA1c)水平,Western blot法检测INR在下丘脑、肝脏、骨骼肌中的表达量。结果:造模后与正常组比较,其余3组大鼠体质量均明显增加(P<0.001),FPG和2 h PG升高(P<0.05,P<0.001),符合IGT的诊断标准,提示造模成功。干预后与正常组比较,模型组大鼠体质量明显增加(P<0.001),FPG、2 h PG及GC、GHbA1c含量明显升高(P<0.05,P<0.01),血浆INS含量及下丘脑、肝脏、骨骼肌INR表达明显降低(P<0.001,P<0.01,P<0.05)。干预后与模型组比较,taVNS组大鼠体质量、FPG、2 h PG、血浆GC含量明显降低(P<0.01,P<0.05),INS含量及下丘脑、肝脏、骨骼肌中INR表达明显升高(P<0.001,P<0.01,P<0.05)。干预后与tnVNS组比较,taVNS组大鼠FPG和2 h PG值降低(P<0.05),INS含量及下丘脑、肝脏中INR表达明显升高(P<0.001,P<0.05,P<0.01)。结论:经皮耳穴-迷走神经刺激可调节IGT模型大鼠的FPG和2 h PG,降低GC,提高INS敏感性,上调下丘脑、肝脏、骨骼肌中INR的表达。
基金Supported by the National Natural Science Foundation of China(No.C30472133,No.30973798 and No.81473780)the Austrian Science Fund(Application No.I2574-B31)
文摘Treatment by auricular acupuncture has a long history. Ear-acupoint research has been advancing step by step in China and also in Europe. Auricles are rich in nerves, therefore a close relationship with different functions of the human body has been proved by the research teams of the two main authors of this article from China and Austria. In recent years, great progress has been made in the research of regulating human body functions through electroacupuncture at the auricular branch of the vagus nerve, which is part of auricular acupuncture therapy. It is well known that the auricular branch of the vagus nerve is the only peripheral pathway to the cerebral cortex. Studies of the Chinese team on hypertension, diabetes, epilepsy and depression have shown that the mechanism of auricular vagus nerve stimulation （VNS） may be comparable with cervical VNS in terms of pathways. Auricular VNS has a broad clinical application prospect.
