Objective To obtain and dig out the transcriptome database of Qushi Huoxue Formula acting on nonalcoholic fatty liver disease(NAFLD)hepatocytes and the differentially expressed genes of pyrolysis related to the inflam...Objective To obtain and dig out the transcriptome database of Qushi Huoxue Formula acting on nonalcoholic fatty liver disease(NAFLD)hepatocytes and the differentially expressed genes of pyrolysis related to the inflammatory environment of hepatocytes.Methods The research used palmitic acidinduced human liver cell line L-02 to establish the cell model of NAFLD.Transcriptome sequencing with Ribosome RNA removed(Ribo-Zero RNA-Seq)was used to transcriptome sequence the L-02 cell model treated by Qushi Huoxue Recipe.Systematic bioinformatics analysis was performed and differentially expressed genes related to inflammatory environment pyroptosis were screened out.展开更多
文摘Objective To obtain and dig out the transcriptome database of Qushi Huoxue Formula acting on nonalcoholic fatty liver disease(NAFLD)hepatocytes and the differentially expressed genes of pyrolysis related to the inflammatory environment of hepatocytes.Methods The research used palmitic acidinduced human liver cell line L-02 to establish the cell model of NAFLD.Transcriptome sequencing with Ribosome RNA removed(Ribo-Zero RNA-Seq)was used to transcriptome sequence the L-02 cell model treated by Qushi Huoxue Recipe.Systematic bioinformatics analysis was performed and differentially expressed genes related to inflammatory environment pyroptosis were screened out.
