利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因...利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因的内含子和外显子结构模式有3种,差异显著。番茄SlSUTs和拟南芥AtSUTs氨基酸都具有2种基序存在形式,且相同基序形式的基序位置存在关系一致。番茄和拟南芥SUTs蛋白的二级结构都有4种,其中α螺旋占比最多,β折叠占比最少,蛋白具有11或12个跨膜结构域,亚细胞定位都在细胞膜上。植物SUTs基因的进化关系遵循物种种属的亲缘关系,番茄SlSUTs基因与葡萄的进化关系更相近。番茄SlSUTs基因在不同组织和果实不同发育时期的表达都不相同,说明SlSUTs基因的表达具有一定的组织特异性。展开更多
[Objective] In order to study the effects of brassinosteroid on salinity toler- ance of cotton. [Method] Three application modes （leaf application, root application and leaf/root application） of brassinosteroid for ...[Objective] In order to study the effects of brassinosteroid on salinity toler- ance of cotton. [Method] Three application modes （leaf application, root application and leaf/root application） of brassinosteroid for cotton under NaCI stress were de- signed to understand the effects of brassinosteroid on Na＋ and CI- accumulation, osmotic adjustment substance （proline） and biomass accumulation of cotton under NaCI stress. [Result] NaCI inhibited the growth of cotton seedlings. Compared with the control group, the biomass of cotton seedlings was decreased, Na＋ and CI- contents, MDA content and proline content were increased in cotton under NaCI stress. Under the NaCI stress, three application modes of brassinosteroid significant- ly improved the biomass, chlorophyll content in leaves, root activity and proline content; while Na~ and CI- content, MDA content were decreased. The enhancement of root activity and root physiological functions were more marked in root application and leaf/root application treatments than in leaf/root application treatment. [Conclu- sion] Three application modes of brassinosteroid all could alleviate the NaCI injuries on cotton, but root application and root/leaf application modes of brassinosteroid for cotton under NaCI stress are superior to the leaf application.展开更多
目的:为了满足高蛋白质藜麦的选育、栽培和农业实践所需,实现藜麦籽粒粗蛋白含量快速、无损检测。方法:本研究应用近红外光谱技术对藜麦籽粒粗蛋白含量的快速检测进行系统研究,选用具有代表性的122份藜麦品种为试材,以其中94份为建模集...目的:为了满足高蛋白质藜麦的选育、栽培和农业实践所需,实现藜麦籽粒粗蛋白含量快速、无损检测。方法:本研究应用近红外光谱技术对藜麦籽粒粗蛋白含量的快速检测进行系统研究,选用具有代表性的122份藜麦品种为试材,以其中94份为建模集,28份为验证集,扫描得到藜麦建模集的近红外原始光谱,用Unscrambler 10.4软件进行光谱预处理并使用偏最小二乘法(PLS)建立藜麦籽粒粗蛋白含量的定量预测模型。结果:经滤波拟合法(Savitzky-Golay,SG)+标准正态变量(Standard Normal Variate,SNV)预处理建立的模型预测值决定系数(R2)为0.9380,被测组分浓度分析误差(RMSE)为0.4823,表现最佳。用此模型对验证集28份样品进行预测,相关分析表明,预测值与国标法实测值决定系数为0.9416;单因素方差分析表明,国标法实测值和模型预测值之间无显著差异(P>0.05),建立的模型具有很高的准确性,预测效果良好。结论:近红外光谱法作为一种简单快速无损的检测手段,能够用于藜麦籽粒粗蛋白含量的检测,可以为优质藜麦育种、栽培和农业实践提供技术支持。展开更多
文摘利用生物信息学方法对番茄SUTs基因家族成员基因及蛋白质序列进行生物信息学分析,并对基因的表达特性进行研究。番茄SlSUTs基因家族共有3个成员,蛋白质的分子质量在54.15~64.99 k D之间;3个基因均匀分布在4号、5号和11号染色体上;基因的内含子和外显子结构模式有3种,差异显著。番茄SlSUTs和拟南芥AtSUTs氨基酸都具有2种基序存在形式,且相同基序形式的基序位置存在关系一致。番茄和拟南芥SUTs蛋白的二级结构都有4种,其中α螺旋占比最多,β折叠占比最少,蛋白具有11或12个跨膜结构域,亚细胞定位都在细胞膜上。植物SUTs基因的进化关系遵循物种种属的亲缘关系,番茄SlSUTs基因与葡萄的进化关系更相近。番茄SlSUTs基因在不同组织和果实不同发育时期的表达都不相同,说明SlSUTs基因的表达具有一定的组织特异性。
基金Supported by National Science Foundation of China(31201139)National R&D Project of Transgenic Crops of Ministry of Science and Technology of China(2011ZX08005001)Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(14)2065)~~
文摘[Objective] In order to study the effects of brassinosteroid on salinity toler- ance of cotton. [Method] Three application modes （leaf application, root application and leaf/root application） of brassinosteroid for cotton under NaCI stress were de- signed to understand the effects of brassinosteroid on Na＋ and CI- accumulation, osmotic adjustment substance （proline） and biomass accumulation of cotton under NaCI stress. [Result] NaCI inhibited the growth of cotton seedlings. Compared with the control group, the biomass of cotton seedlings was decreased, Na＋ and CI- contents, MDA content and proline content were increased in cotton under NaCI stress. Under the NaCI stress, three application modes of brassinosteroid significant- ly improved the biomass, chlorophyll content in leaves, root activity and proline content; while Na~ and CI- content, MDA content were decreased. The enhancement of root activity and root physiological functions were more marked in root application and leaf/root application treatments than in leaf/root application treatment. [Conclu- sion] Three application modes of brassinosteroid all could alleviate the NaCI injuries on cotton, but root application and root/leaf application modes of brassinosteroid for cotton under NaCI stress are superior to the leaf application.
文摘目的:为了满足高蛋白质藜麦的选育、栽培和农业实践所需,实现藜麦籽粒粗蛋白含量快速、无损检测。方法:本研究应用近红外光谱技术对藜麦籽粒粗蛋白含量的快速检测进行系统研究,选用具有代表性的122份藜麦品种为试材,以其中94份为建模集,28份为验证集,扫描得到藜麦建模集的近红外原始光谱,用Unscrambler 10.4软件进行光谱预处理并使用偏最小二乘法(PLS)建立藜麦籽粒粗蛋白含量的定量预测模型。结果:经滤波拟合法(Savitzky-Golay,SG)+标准正态变量(Standard Normal Variate,SNV)预处理建立的模型预测值决定系数(R2)为0.9380,被测组分浓度分析误差(RMSE)为0.4823,表现最佳。用此模型对验证集28份样品进行预测,相关分析表明,预测值与国标法实测值决定系数为0.9416;单因素方差分析表明,国标法实测值和模型预测值之间无显著差异(P>0.05),建立的模型具有很高的准确性,预测效果良好。结论:近红外光谱法作为一种简单快速无损的检测手段,能够用于藜麦籽粒粗蛋白含量的检测,可以为优质藜麦育种、栽培和农业实践提供技术支持。
