To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala...To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala tympani of the animals who had been systemically sensitized. The expression of ICAM-1 in the inner ear was immunohistochemically examined. ICAM-1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules (CVs) as early as 6 h after challenge. Expression of ICAM-1 was observed on the epithelium of the endolymphatic sac (ES) and perisaccular region at 12 h. The intensity of ICAM-1 staining reached its peak within 24 - 48 h in these sites of the inner ear. By day 28, most specimens were devoid of appreciable staining for ICAM-1. Our study demonstrates that adhesion molecules play an important role in extravasation of inflammatory cells from the systemic circulation in the process of inner ear immune response. It also shows that cytokines that control expression of adhesion molecules may be released by cells outside ES, besides those cells in the ES.展开更多
Objective To investigate the role of peripheral immunocompetent lymphocytes during the immune response in the inner ear Methods Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen of three g...Objective To investigate the role of peripheral immunocompetent lymphocytes during the immune response in the inner ear Methods Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen of three groups of donor animals, i e , group A immunized intradermally with keyhole limpet hemocyanine (KLH) and both inner ears challenged with KLH, group B received only KLH intradermally without inner ear challenge, and group C used as control, were isolated and labelled with 51 Cr The labelled lymphocytes were injected intravenously into the recipients They were immunized intradermally with right inner ears challenged with KLH Twenty to 24?h later, the radioactivities in the temporal bones of the recipients were assayed to detect the infiltration of labelled cells Results In groups A and B, the radioactivity in the right temporal bones was higher than that in the left temporal bones (P<0 05) The highest labelling radioactivity was detected in the right temporal bones of group A The lowest migration of 51 Cr labelled cells to the inner ear was found in group C The ratio of radioactivity between right (challenged) and left (unchallenged) inner ears was higher in group A than in groups B and C, with the difference being significant (P<0 05) Conclusion The immune reaction in response to antigenic stimulation in the inner ear is immunologically modulated by the peripheral systemic circulation展开更多
基金This project was supported by a grant from the National Natural Science Foundation of China (No.39400146)
文摘To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala tympani of the animals who had been systemically sensitized. The expression of ICAM-1 in the inner ear was immunohistochemically examined. ICAM-1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules (CVs) as early as 6 h after challenge. Expression of ICAM-1 was observed on the epithelium of the endolymphatic sac (ES) and perisaccular region at 12 h. The intensity of ICAM-1 staining reached its peak within 24 - 48 h in these sites of the inner ear. By day 28, most specimens were devoid of appreciable staining for ICAM-1. Our study demonstrates that adhesion molecules play an important role in extravasation of inflammatory cells from the systemic circulation in the process of inner ear immune response. It also shows that cytokines that control expression of adhesion molecules may be released by cells outside ES, besides those cells in the ES.
文摘Objective To investigate the role of peripheral immunocompetent lymphocytes during the immune response in the inner ear Methods Sensitized lymphocytes from peripheral blood, neck lymph nodes and spleen of three groups of donor animals, i e , group A immunized intradermally with keyhole limpet hemocyanine (KLH) and both inner ears challenged with KLH, group B received only KLH intradermally without inner ear challenge, and group C used as control, were isolated and labelled with 51 Cr The labelled lymphocytes were injected intravenously into the recipients They were immunized intradermally with right inner ears challenged with KLH Twenty to 24?h later, the radioactivities in the temporal bones of the recipients were assayed to detect the infiltration of labelled cells Results In groups A and B, the radioactivity in the right temporal bones was higher than that in the left temporal bones (P<0 05) The highest labelling radioactivity was detected in the right temporal bones of group A The lowest migration of 51 Cr labelled cells to the inner ear was found in group C The ratio of radioactivity between right (challenged) and left (unchallenged) inner ears was higher in group A than in groups B and C, with the difference being significant (P<0 05) Conclusion The immune reaction in response to antigenic stimulation in the inner ear is immunologically modulated by the peripheral systemic circulation
