VP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones ...VP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones were sequenced with sequencing kit ABI PRISM by TaKaRa CoLtdThe open reading frame of the VP1 gene is made up of 1,350bpIt encodes 449 amino acid residuesIt was found that there is an abundant Arg region from position 3 to 46Its isoelectric point is up to 107 because of its abundant basic amino acidsComparing this Harbin isolate with 26p4,Cux-1,82-2,Del-rose,A2,Connb,L-028,TR20,CIA-1,CAV15,2A9 showed there were 168 nucleoside differences which lead to 26 amino acid changes of the VP1The amino acid changes may influence the antigenic character of different VP1sIt was also found that there is a hyper-mutation region from amino acids position 139-157,its mutation frequency is up to 42% among these CAV’s VP1s.It is necessary to research the function of this展开更多
文摘VP1 gene of chicken anemia virus in liver of infected chicken from Harbin was amplified by polymerase chain reaction and cloned into pUC18The recombinant vector was identified by restriction digestionThree clones were sequenced with sequencing kit ABI PRISM by TaKaRa CoLtdThe open reading frame of the VP1 gene is made up of 1,350bpIt encodes 449 amino acid residuesIt was found that there is an abundant Arg region from position 3 to 46Its isoelectric point is up to 107 because of its abundant basic amino acidsComparing this Harbin isolate with 26p4,Cux-1,82-2,Del-rose,A2,Connb,L-028,TR20,CIA-1,CAV15,2A9 showed there were 168 nucleoside differences which lead to 26 amino acid changes of the VP1The amino acid changes may influence the antigenic character of different VP1sIt was also found that there is a hyper-mutation region from amino acids position 139-157,its mutation frequency is up to 42% among these CAV’s VP1s.It is necessary to research the function of this
