This study is the first to describe and illustrate the dinoflagellate genus Dicroerisma in the open Pacific Sea and in the southern hemisphere. Eleven individuals ascribed to Dicroerisma psilonereiella were encountere...This study is the first to describe and illustrate the dinoflagellate genus Dicroerisma in the open Pacific Sea and in the southern hemisphere. Eleven individuals ascribed to Dicroerisma psilonereiella were encountered in subsurface waters (>70 m depth) from 34°N to 33°S. Another specimen that differs from the type is also illustrated. These findings reveal that Dicroerisma is a widespread genus and that there is tentative existence of another species within the genus.展开更多
目的:评价手法治疗对兔膝关节软骨浅层和深层缺损修复的效果。方法:将24只新西兰兔随机分成手法组和对照组。分别在24只兔双侧膝关节的股骨内侧髁造成直径2mm的浅层软骨缺损,在股骨髌面造成直径3mm的深层软骨缺损。造模4周后,手法组采...目的:评价手法治疗对兔膝关节软骨浅层和深层缺损修复的效果。方法:将24只新西兰兔随机分成手法组和对照组。分别在24只兔双侧膝关节的股骨内侧髁造成直径2mm的浅层软骨缺损,在股骨髌面造成直径3mm的深层软骨缺损。造模4周后,手法组采取手法治疗,对照组不采取任何治疗措施。分别于治疗后4、8、12周取材,对缺损区域的修复组织进行外观形态、苏木精和伊红(hematoxylin and eosin,HE)染色、番红O染色以及Ⅱ型胶原免疫组织化学染色观察,并采用O'Driscoll软骨组织形态学评分评价软骨缺损的修复质量。结果:治疗后,手法组软骨浅层缺损和软骨深层缺损渐渐修复。修复组织质地稍软,表面光滑,外观与正常软骨类似,大部分区域与周围正常软骨界限模糊,与周围软骨整合良好。对照组浅层缺损虽然有部分被修复,但所修复组织与周围软骨界限清晰;深层缺损只是缺损面积略减小,未见到缺损修复,或以纤维样组织填充。HE染色显示手法组修复组织内有大量的透明软骨细胞,番红O染色显示手法组修复组织基质染色接近周围正常关节软骨,对照组无染色。Ⅱ型胶原免疫组织化学染色显示,手法组浅层缺损和深层缺损的修复组织Ⅱ型胶原染色与正常软骨相比染色正常甚至较深;对照组浅层和深层缺损的修复组织无染色,修复组织与正常组织界限清晰。O'Driscoll评分结果显示,手法组的浅层缺损和深层缺损的评分均高于同一时间段对照组,差异有统计学意义(P<0.01);治疗后,手法组无论浅层缺损还是深层缺损,各时间点ODriscoll评分比较,差异均无统计学意义(P>0.05)。结论:手法治疗能有效地修复兔膝关节的浅层软骨缺损和深层软骨缺损,修复物质与透明软骨相似。展开更多
基金Supported by a fellowship of the European Commission (ICB2-CT-2001-80002).
文摘This study is the first to describe and illustrate the dinoflagellate genus Dicroerisma in the open Pacific Sea and in the southern hemisphere. Eleven individuals ascribed to Dicroerisma psilonereiella were encountered in subsurface waters (>70 m depth) from 34°N to 33°S. Another specimen that differs from the type is also illustrated. These findings reveal that Dicroerisma is a widespread genus and that there is tentative existence of another species within the genus.
文摘目的:评价手法治疗对兔膝关节软骨浅层和深层缺损修复的效果。方法:将24只新西兰兔随机分成手法组和对照组。分别在24只兔双侧膝关节的股骨内侧髁造成直径2mm的浅层软骨缺损,在股骨髌面造成直径3mm的深层软骨缺损。造模4周后,手法组采取手法治疗,对照组不采取任何治疗措施。分别于治疗后4、8、12周取材,对缺损区域的修复组织进行外观形态、苏木精和伊红(hematoxylin and eosin,HE)染色、番红O染色以及Ⅱ型胶原免疫组织化学染色观察,并采用O'Driscoll软骨组织形态学评分评价软骨缺损的修复质量。结果:治疗后,手法组软骨浅层缺损和软骨深层缺损渐渐修复。修复组织质地稍软,表面光滑,外观与正常软骨类似,大部分区域与周围正常软骨界限模糊,与周围软骨整合良好。对照组浅层缺损虽然有部分被修复,但所修复组织与周围软骨界限清晰;深层缺损只是缺损面积略减小,未见到缺损修复,或以纤维样组织填充。HE染色显示手法组修复组织内有大量的透明软骨细胞,番红O染色显示手法组修复组织基质染色接近周围正常关节软骨,对照组无染色。Ⅱ型胶原免疫组织化学染色显示,手法组浅层缺损和深层缺损的修复组织Ⅱ型胶原染色与正常软骨相比染色正常甚至较深;对照组浅层和深层缺损的修复组织无染色,修复组织与正常组织界限清晰。O'Driscoll评分结果显示,手法组的浅层缺损和深层缺损的评分均高于同一时间段对照组,差异有统计学意义(P<0.01);治疗后,手法组无论浅层缺损还是深层缺损,各时间点ODriscoll评分比较,差异均无统计学意义(P>0.05)。结论:手法治疗能有效地修复兔膝关节的浅层软骨缺损和深层软骨缺损,修复物质与透明软骨相似。
