摘要
【目的】柑橘大实蝇是一种严重为害柑橘类果实的经济害虫。研究其精巢、精泵以及精泵内骨骼生长发育状况,有助于提高柑橘大实蝇人工繁殖效率以及其田间防治效果,为柑橘大实蝇的预测预报及防治提供理论基础。【方法】基于光学显微镜测量恒温和室温条件下柑橘大实蝇雄虫的精巢、精泵以及精泵内骨骼的长度和宽度,并建立其长度、宽度和指数的函数模型,比较恒温和室温2种饲养条件下其雄虫器官发育状况差异。【结果】无论是在恒温还是室温条件下饲养,随着柑橘大实蝇雄成虫日龄的增加,其精巢、精泵以及精泵内骨骼的长度、宽度和指数变化趋势均符合幂函数增长模型。在恒温和室温条件下,其雄虫的精巢宽度、精泵的长度和宽度以及指数、精泵内骨骼宽度均无明显差异。在室温条件下饲养的雄虫的精巢长度[(4.00±0.14)mm]及指数(3.40±0.14)、精泵内骨骼长度[(1.65±0.03)mm]及指数(1.84±0.08)均分别显著高于在恒温条件下饲养的雄虫的精巢长度[(3.75±0.13)mm]及指数(3.19±0.14)、精泵内骨骼长度[(1.61±0.03)mm]及指数(1.77±0.08)。【结论】变温(室温)比恒温更有利于柑橘大实蝇雄虫的精巢和精泵内骨骼的发育,并推荐使用精泵长度推断其雄虫日龄的方法。
【Aim】The Chinese citrus fly,Bactrocera minax(Enderlein)(Diptera:Tephritidae),is an economic pest seriously damaging citrus fruits.Currently,there is a limited body of research on the growth and development of the testis,ejaculatory pump,and ejaculatory pump endoskeleton.【Method】We measured the length and width of the testis,ejaculatory pump,and ejaculatory pump endoskeleton of the male adult B.minax using an optical microscope under constant and room temperature rearing conditions.We established functional models for length,width,and index,comparing the differences in organ development between two rearing conditions.【Result】In both constant temperature and room temperature rearing,the growth patterns of the testis(length,width,and index),ejaculatory pump,and ejaculatory pump endoskeleton followed a power function growth model with the passage of days.No significant differences exist in testis width,ejaculatory pump endoskeleton width,or the length,width,and index of the ejaculatory pump between male adults reared under constant temperature and room temperature conditions.However,the length(4.00±0.14)mm and index(3.40±0.14)of the testis and the length(1.65±0.03)mm and index(1.84±0.08)of the ejaculatory pump endoskeleton in male adults reared at room temperature were significantly higher than the length(3.75±0.13)mm and index(3.19±0.14)of the testis and the length(1.61±0.03)mm and index(1.77±0.08)of the ejaculatory pump endoskeleton in male adults reared at constant temperature.【Conclusion】Variable temperature,specifically room temperature,appears to be more conducive to developing testis and ejaculatory pump endoskeleton in male B.minax than a constant temperature.Using ejaculatory pump length to infer the age of male B.minax can improve the efficiency of artificial reproduction and field control effects,providing a theoretical basis for predicting and preventing B.minax infestations.
作者
汤建涛
刘文茹
桂连友
刘梅柯
周仁迪
华登科
姜振宇
孙康林
张国辉
王裕雷
蔡开源
TANG Jiantao;LIU Wenru;GUI Lianyou;LIU Meike;ZHOU Rendi;HUA Dengke;JIANG Zhenyu;SUN Kanglin;ZHANG Guohui;WANG Yulei;CAI Kaiyuan(Forewarning and Management of Agricultural and Forestry Pests,Hubei Engineering Technology Center,College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China;Institute of Agricultural Quality Standards and Testing Technology,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064,China)
出处
《生物安全学报》
CSCD
北大核心
2023年第4期333-340,共8页
Journal of biosafety
基金
国家自然科学基金(31772206、31972274)。
关键词
柑橘大实蝇
精巢
精泵
精泵内骨骼
光学显微法
Bactrocera minax
testis
ejaculatory pump
ejaculatory pump endoskeleton
optical microscopy
