摘要
本文建立了自少量血清中研究I-IBsAg多肽图谱的方法,患者血清经纯化、SD.S聚丙烯酰胺凝胶电泳及Western吸印后,HBgAg多肽用酶免疫染色或放射自显影进行分析,发现10例慢性乙肝患者血清HBsAg多肽组成有明显差别.同一来源的HBV毒株在不同个体或同一个体随病程不同多肽图谱亦不同.血清HBV DNA阳性与多肽P39/P42的关系较P31/P34为密切,提示Pre-S1抗原不仅在急性感染中与病毒复制有关,在慢性感染中亦有关,PHSA'受体与P31/P42的符合率为94.1%,反映这一多肽确带有PHSA受体.
A method of isolating HBsAg from small aniot of serum for polypeptide pattern analysis was established.7-14 ug of HBsAg was urifed through anti-HBs affnity chromatography.Followed by SDS-PAGE and transferred to nitrocellulose filters by Western blot.Filters were further stained with guinea pig anti-HBs,biotinylated anti-guinea pig IgG and avidinbiotin complex;or by 125I-SPA and autoradiOgraphy.Distinct polypeptide bands coald be seen.Serum samples from 10 chronic hepatitis B patients showed different polypepti le patterns.P31/P34 bands were seen in 9,and 3 showed high molecular woight band(P1OO).In the follow-up study of the 5 chronic hepatitis B patients,4 showed significant changes in patterns,some migrated from high to low molecular weight bands, while some changed from low to high molecular weight ban&s.In the 2 paired serum samples obtained from accidental transmission.HBsAg polypeptide bands of the donors and their relative recipients were found different.The data indicated that among the serum HBV DNA positive carriers,59%sjpwed P39/P42,whereas none of the HBV DNA negative carriers had such bands.In contrast.P31/P34 appeared both the serum HBV DNA positive and negative carriers.It seems that in tIBsAg positive carriers,P39/P42 is more closely related to HBV replication.
出处
《中华传染病杂志》
CAS
1987年第4期193-197,后插1,共6页
Chinese Journal of Infectious Diseases
