摘要
<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Tuberculosis is a health problem in Sudan and may become a greater challenge in the future due to the weakness in infection prevention measures, increase in cases of drug-resistant and the difficulty of diagnosis.</span><b><span style="font-family:Verdana;"> Objective: </span></b><span style="font-family:Verdana;">The aim of this study was to detect </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> (MTB) from sputum of clinically suspected patients using the three available techniques.</span><b><span style="font-family:Verdana;"> Methods:</span></b><span style="font-family:Verdana;"> Three</span><b> </b><span style="font-family:Verdana;">hundred participants referred to Wad Madani Tuberculosis Center during 2017-2019 were included. Early morning fresh sputum samples were subjected to </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> examination by Ziehl-Neelsen</span><b> </b><span style="font-family:Verdana;">(ZN) stain without concentration, ZN stain with centrifugation and geneXpert assay. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">Of the 300 suspected cases;</span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis </span></i><span style="font-family:Verdana;">detected in 17% (51/300) by ZN stain</span><b> </b><span style="font-family:Verdana;">without concentration, 20% (59/300) by ZN stain with centrifugation and 34% (103/300) by geneXpert. The two techniques of ZN stains possessed 100% specificity and relative differences in sensitivity when compared to geneXpert assay. The significant association observed between ZN stains and geneXpert results indicated validity of ZN techniques for detection. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><i> </i><span style="
<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Tuberculosis is a health problem in Sudan and may become a greater challenge in the future due to the weakness in infection prevention measures, increase in cases of drug-resistant and the difficulty of diagnosis.</span><b><span style="font-family:Verdana;"> Objective: </span></b><span style="font-family:Verdana;">The aim of this study was to detect </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> (MTB) from sputum of clinically suspected patients using the three available techniques.</span><b><span style="font-family:Verdana;"> Methods:</span></b><span style="font-family:Verdana;"> Three</span><b> </b><span style="font-family:Verdana;">hundred participants referred to Wad Madani Tuberculosis Center during 2017-2019 were included. Early morning fresh sputum samples were subjected to </span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis</span></i><span style="font-family:Verdana;"> examination by Ziehl-Neelsen</span><b> </b><span style="font-family:Verdana;">(ZN) stain without concentration, ZN stain with centrifugation and geneXpert assay. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">Of the 300 suspected cases;</span><i><span style="font-family:Verdana;">Mycobacterium tuberculosis </span></i><span style="font-family:Verdana;">detected in 17% (51/300) by ZN stain</span><b> </b><span style="font-family:Verdana;">without concentration, 20% (59/300) by ZN stain with centrifugation and 34% (103/300) by geneXpert. The two techniques of ZN stains possessed 100% specificity and relative differences in sensitivity when compared to geneXpert assay. The significant association observed between ZN stains and geneXpert results indicated validity of ZN techniques for detection. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><i> </i><span style="
作者
Zeinab Hamid Elfaham
Elamin M. Ibrahim
Elhadi A. Ahmed
Abdelrhman E. Mohammed
Bakri Y. M. Nour
Zeinab Hamid Elfaham;Elamin M. Ibrahim;Elhadi A. Ahmed;Abdelrhman E. Mohammed;Bakri Y. M. Nour(Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan;Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan)
