摘要
An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"><sup></sup></span><sup></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;"><sup>–ΔΔCт</sup><span style="white-space:nowrap;"></span>) w
An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"><sup></sup></span><sup></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;"><sup>–ΔΔCт</sup><span style="white-space:nowrap;"></span>) w
