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<i>In Vitro</i>Clonal Propagation from Juvenile and Different Explant Types of Two Edible <i>Annonaceae</i>Species: <i>Annona muricata</i>L. and <i>Annona squamosa</i>L. 被引量:1

<i>In Vitro</i>Clonal Propagation from Juvenile and Different Explant Types of Two Edible <i>Annonaceae</i>Species: <i>Annona muricata</i>L. and <i>Annona squamosa</i>L.
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摘要 <i><span style="font-family:"">Annona</span></i><span style="font-family:""> <i>muricata</i> L. and <i>Annona</i> <i>squamosa</i> L. are tropical species whose</span><span style="font-family:""> fleshy fruit is edible. They offer real possibilities for socio-economic use, particularly in the fields of medicine, nutrition, ecosystem conservation and the poverty alleviation. This study was set up to evaluate different methods of micropropagation from juvenile material for the regeneration of these species. Thus, MS medium supplemented with [BAP 2 mg&middot;L<sup>-1</sup>] <i>i.e.</i> M2 produced 2.87 newly <span>formed shoots from the cotyledonary nodes of <i>A.</i> <i>muricata</i>. For the terminal apices of <i>A.</i> <i>squamosa</i>, it was MMS medium supplemented with [BAP 2</span> mg&middot;L<sup>-1</sup>] <i>i.e.</i> MM2 that was most conducive to new shoot formation (3.12). The addition of 0.1 and 0.2 mg&middot;L<sup>-1</sup> of NAA in the M2 medium, made it possi<span>ble to have the best elongations and average number of nodes for the new shoots from cotyledonary nodes of <i>A.</i> <i>muricata</i> (9.11 cm for 5.62 nodes). With <i>A.</i> <i>squamosa</i>, MM7 medium [MMS + BAP 1 mg&middot;L<sup>-1</sup></span></span><span style="font-family:""> </span><span style="font-family:"">+ KIN 1 mg&middot;L<sup>-1</sup>]</span><span style="font-family:""> resulted in an average length of 9.05 cm with 5.62 nodes on average for the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50 mg&middot;L<sup>-1</sup>] and 2 g&middot;L<sup>-1</sup> of activated charcoal gave a rooting rate of 66.67% for shoots originating from the cotyledonary nodes of <i>A.</i> <i>squamosa</i>;while with vitroplants from cotyledonary nodes of <i>A.</i> <i>muricata</i>, a better rooting rate (83.33%) was obtained following a 5-day rhizogenic induction. After 30 days of acclimatization, the survival rate reached 83.33% for plants from the tips of <i>A.</i> <i>muricata</i>, whereas for <i>A.</i> <i>squamosa,</i> it was plant <i><span style="font-family:"">Annona</span></i><span style="font-family:""> <i>muricata</i> L. and <i>Annona</i> <i>squamosa</i> L. are tropical species whose</span><span style="font-family:""> fleshy fruit is edible. They offer real possibilities for socio-economic use, particularly in the fields of medicine, nutrition, ecosystem conservation and the poverty alleviation. This study was set up to evaluate different methods of micropropagation from juvenile material for the regeneration of these species. Thus, MS medium supplemented with [BAP 2 mg&middot;L<sup>-1</sup>] <i>i.e.</i> M2 produced 2.87 newly <span>formed shoots from the cotyledonary nodes of <i>A.</i> <i>muricata</i>. For the terminal apices of <i>A.</i> <i>squamosa</i>, it was MMS medium supplemented with [BAP 2</span> mg&middot;L<sup>-1</sup>] <i>i.e.</i> MM2 that was most conducive to new shoot formation (3.12). The addition of 0.1 and 0.2 mg&middot;L<sup>-1</sup> of NAA in the M2 medium, made it possi<span>ble to have the best elongations and average number of nodes for the new shoots from cotyledonary nodes of <i>A.</i> <i>muricata</i> (9.11 cm for 5.62 nodes). With <i>A.</i> <i>squamosa</i>, MM7 medium [MMS + BAP 1 mg&middot;L<sup>-1</sup></span></span><span style="font-family:""> </span><span style="font-family:"">+ KIN 1 mg&middot;L<sup>-1</sup>]</span><span style="font-family:""> resulted in an average length of 9.05 cm with 5.62 nodes on average for the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50 mg&middot;L<sup>-1</sup>] and 2 g&middot;L<sup>-1</sup> of activated charcoal gave a rooting rate of 66.67% for shoots originating from the cotyledonary nodes of <i>A.</i> <i>squamosa</i>;while with vitroplants from cotyledonary nodes of <i>A.</i> <i>muricata</i>, a better rooting rate (83.33%) was obtained following a 5-day rhizogenic induction. After 30 days of acclimatization, the survival rate reached 83.33% for plants from the tips of <i>A.</i> <i>muricata</i>, whereas for <i>A.</i> <i>squamosa,</i> it was plant
作者 Oumar Ba Abraham Dieme Amadou Lamine Ndoye Mame Ourèye Sy Oumar Ba;Abraham Dieme;Amadou Lamine Ndoye;Mame Ourèye Sy(Laboratoire Campus de Biotechnologies Vegetales, Departement de Biologie Vegetale, Faculte des Sciences & Techniques, Universite Cheikh Anta Diop, Dakar, Senegal;Laboratoire Mixte International-Adaptation des Plantes et Microorganismes associes aux Stress Environnementaux (LMI-LAPSE), IRD, ISRA, UCAD, Dakar, Senegal)
出处 《Advances in Bioscience and Biotechnology》 2021年第12期458-480,共23页 生命科学与技术进展(英文)
关键词 <i>Annona muricata Annona squamosa</i> Juvenile Material Micropropagation 6-BENZYLAMINOPURINE 6-Furfuryl Aminopurine 1-Naphthaleneacetic Acid Indole-3-Butyric Acid <i>Annona muricata Annona squamosa</i> Juvenile Material Micropropagation 6-Benzylaminopurine 6-Furfuryl Aminopurine 1-Naphthaleneacetic Acid Indole-3-Butyric Acid
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