摘要
<strong>Background:</strong> Androgenetic alopecia (AGA) is a most common condition of hair loss. Thus, the present study aimed to investigate the effect of Trichoxidil<sup>TM</sup>, a phytocomplex obtained from a blend of essential oils, in the treatment of hair loss caused by AGA. <strong>Methods:</strong> The CCD1072Sk cells were cultured for the 24-hour cell viability assessment and cytotoxicity of Trichoxidil<sup>TM</sup>. The expression of mRNA levels from KGF, IGF-1, and VEGF in fibroblasts was evaluated by RT-qPCR. Thirty-three volunteers, diagnosed with AGA, men and women, aged between 25 and 50 years, were divided into Control group, without treatment (n = 5);Trichosol<sup>TM</sup> vehicle group, without active (n = 5);Hydroalcoholic vehicle group, without active (n = 4);Trichosol<sup>TM</sup> vehicle group, with 2.5% minoxidil (n = 5);Hydroalcoholic vehicle group, with 2.5% minoxidil (n = 5);Trichosol<sup>TM</sup> group with 2.5% Trichoxidil<sup>TM</sup> (n = 5) and Hydroalcoholic vehicle group with 2.5% Trichoxidil<sup>TM</sup> (n = 4) to dermoscopic and histologic. <strong>Results:</strong> Fibroblasts exhibited higher proliferation when treated with higher concentrations of Trichoxidil<sup>TM</sup>. Trichoxidil<sup>TM</sup> significantly increased the expression of KGF, IGF-1, and VEGF mRNA in fibroblasts cells. Analysis of the capillary density showed that Trichoxidil<sup>TM</sup> associated with Trichosol<sup>TM</sup> vehicle, was the most effective association. In addition, it was observed an increased more effectively the percentage of anagen phase and reduction of the telogen when compared to other formulations. <strong>Conclusion:</strong> Trichoxidil<sup>TM</sup> promoted proliferative effects and positively modulated the expression of growth factors IGF-1, VEGF, and KGF, being a promising candidate for the treatment of hair loss caused by AGA.
<strong>Background:</strong> Androgenetic alopecia (AGA) is a most common condition of hair loss. Thus, the present study aimed to investigate the effect of Trichoxidil<sup>TM</sup>, a phytocomplex obtained from a blend of essential oils, in the treatment of hair loss caused by AGA. <strong>Methods:</strong> The CCD1072Sk cells were cultured for the 24-hour cell viability assessment and cytotoxicity of Trichoxidil<sup>TM</sup>. The expression of mRNA levels from KGF, IGF-1, and VEGF in fibroblasts was evaluated by RT-qPCR. Thirty-three volunteers, diagnosed with AGA, men and women, aged between 25 and 50 years, were divided into Control group, without treatment (n = 5);Trichosol<sup>TM</sup> vehicle group, without active (n = 5);Hydroalcoholic vehicle group, without active (n = 4);Trichosol<sup>TM</sup> vehicle group, with 2.5% minoxidil (n = 5);Hydroalcoholic vehicle group, with 2.5% minoxidil (n = 5);Trichosol<sup>TM</sup> group with 2.5% Trichoxidil<sup>TM</sup> (n = 5) and Hydroalcoholic vehicle group with 2.5% Trichoxidil<sup>TM</sup> (n = 4) to dermoscopic and histologic. <strong>Results:</strong> Fibroblasts exhibited higher proliferation when treated with higher concentrations of Trichoxidil<sup>TM</sup>. Trichoxidil<sup>TM</sup> significantly increased the expression of KGF, IGF-1, and VEGF mRNA in fibroblasts cells. Analysis of the capillary density showed that Trichoxidil<sup>TM</sup> associated with Trichosol<sup>TM</sup> vehicle, was the most effective association. In addition, it was observed an increased more effectively the percentage of anagen phase and reduction of the telogen when compared to other formulations. <strong>Conclusion:</strong> Trichoxidil<sup>TM</sup> promoted proliferative effects and positively modulated the expression of growth factors IGF-1, VEGF, and KGF, being a promising candidate for the treatment of hair loss caused by AGA.
